不同日龄苏太仔猪LTβR基因组织表达谱及发育性表达规律分析

doi:10.16431/j.cnki.1671-7236.2016.04.025

Abstract

Abstract: Dynamic changes of LTβR expression levels in 11 tissues (heart, liver, spleen, lung, kidney, stomach, muscle, thymus, lymph node, duodenum and jejunum) of Sutai piglets ranging from newborn to post-weaning days 8, 18, 30, and 35 were compared and analyzed by the Real-time PCR method, which aimed to provide theoretical basis for further investigate the relationship between LTβR gene and pathogenicity of E.coli F18.The results revealed that the LTβR expression levels were higher in the liver, spleen, lung, kidney, stomach, lymph node, duodenum and jejunum, and showed obvious age-dependent expression differentiation.The LTβR expression levels in the lymph node, duodenum, and jejunum were extremely significant higher in 8 days old piglets than in the other age stages (P<0.01), and the expression levels were extremely significantly higher in the lungs of 8 days old piglets than in 35 days old piglets (P<0.01) and significantly higher than 30 days old piglets (P<0.05).In the liver tissue, the expression level was extremely significant higher in 35 days old piglets than in other age stages (P<0.01).In the stomach tissue, the expression level was significantly higher in 35 days old piglets than in 18 days old piglets (P<0.05).The results speculated that intestinal immune barrier of piglets formed rapidly around 8 days old and the higher LTβR expression could contribute to the resistance to E.coli F18.

Key words: pigs; LTβR gene; E.coli F18; differential expression

CLC Number:

Q786

XIA Ri-wei, GAN Li-na, QIN Wei-yun, SUN Shou-yong, BAO Wen-bin, WU Sheng-long. Tissue Expression Profile and Differential Expression of LTβR Gene in Sutai Piglets of Different Ages[J]. , 2016, 43(4): 1017-1023.

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Tissue Expression Profile and Differential Expression of LTβR Gene in Sutai Piglets of Different Ages

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