洱源、怒江猫源弓形虫虫株的分离与鉴定

doi:10.16431/j.cnki.1671-7236.2016.03.038

Abstract

Abstract: The assay was aimed to isolate Toxoplasma gondii (T.gondii) strains from stray cat in Eryuan and Nujiang of Yunnan province.The cat tissues (heart,liver,lung and brain) were digested by acid pepsin solution,intraperitoneally inoculated in Kunming mice,passaged at least 3 generations,and followed by specific PCR amplification of partial B1 gene using species-specific primers.Three T.gondii isolates were isolated from 18 stray cats,PCR result showed that we got the specific target band,and the sequence result of the specific PCR product showed that it was ribosome B1 gene sequence of T.gondii. Homology comparison analysis showed that the isolates was 100.0% homology with T.gondii B1.The method of inoculation into the mice with the tissues that was digested by acid pepsin solution was an effective way to isolate T.gondii strain from animals,and the specific PCR assay was an accurate method for the rapid identification of T.gondii.

Key words: Toxoplasma gondii; stray cat; B1 gene; PCR identification

CLC Number:

S852.7

LI Hao-xin, YAN Hong-ya, DUAN Gang, XIANG Xun, ZHU Qi, YANG Zhi-yuan, ZU Fei, CHANG Hua. Isolation and Identification of Toxoplasma gondii Isolates from Cats in Eryuan and Nujiang[J]. , 2016, 43(3): 825-830.

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Isolation and Identification of Toxoplasma gondii Isolates from Cats in Eryuan and Nujiang

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