广西部分地区猪链球菌的分离鉴定及小鼠致病性研究

doi:10.16431/j.cnki.1671-7236.2017.03.034

Abstract

Abstract:

16 strains of Streptococcus were isolated and identified from 370 pig tissues of clinically morbidity farms and slaughter houses in Guangxi border area.Morphological observation, PCR identification and pathogenicity test were carried out. The results showed that the morphology,dyeing,biochemical characteristics were coincidence with the characteristics of Streptococcus suis,confirmed that 16 strains were Streptococcus suis,and named as GX1 to GX16. Further by divided type identification (35 serotypes) by GDH,1 strains was type 1,1 strains was type 2,1 strains was type 5,1 strain was type 29,5 strains were type 8 and 7 strains could not differentiate types. The pathogenicity of 16 strains Streptococcus suis were detected by Kunming mice and BALB/c mice. The results showed that the letal rate of GX10 for Kunming mice and BALB/c mice was 100%;GX11 for BALB/c mice was 80%;Other strains were apathogenicity to the Kunming mice and BALB/c mice. The LD₅₀ of GX10 and GX11 was 4.5×10⁵ and 3.6×10⁹ CFU for BALB/c mice,respectively. The results showed that pathogenic Streptococcus suis type 8 occured in Guangxi border area. The sensitivity of BALB/c mice to Streptococcus suis was higher than Kunming mice, BALB/c mice was more suitable for the pathogenic study of Streptococcus suis.

Key words: Streptococcus suis; PCR identification; animal pathogenicity test; LD₅₀

CLC Number:

S858.28

CHEN Wen-wen, WEI Xian-kai, BI Jing-shan, ZHENG Min, SU Jiao-xiu, CAO Hui-hui, ZHANG Hong-yun, ZHENG Lie-feng, LIU Qi. Isolation, Identification and Pathogenicity on Mice of Streptococcus suis in Pig in Guangxi Border Area[J]. , 2017, 44(3): 861-867.

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Isolation, Identification and Pathogenicity on Mice of Streptococcus suis in Pig in Guangxi Border Area

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