扭角羚肺炎克雷伯氏菌的分离鉴定

doi:10.16431/j.cnki.1671-7236.2015.01.032

Abstract

Abstract: In order to report the detection and main biological characteristics of pathogenic bacteria isolated from wild takin in the process of the natural survival, four strains of the predominant bacteria from pathologic tissues of dead takin's livers, spleens and lungs were examined and isolated.The detections of biochemical characteristics and 16S rDNA gene were conducted.The strains were isolated and identified as K.pneumoniae.The four isolates had pathogenicity to mice.LD₅₀ of the four isolates were 2.9×10⁷, 2.9×10⁷, 4.5×10⁷ and 1.1×10⁸ CFU, respectively.The antibiotic sensitivity test revealed that the strains were sensitive to cephalothin and so on, moderate sensitive to amikacin and so on, and resistant to erythrocin and so on.The four strains of K.pneumoniae were the primary bacterial pathogen leading to the death of takin.

Key words: takin; K.pneumoniae; isolation and identification; LD₅₀

CLC Number:

S852.61

HAO Zhong-xiang, LIAO Hong, LIU Dan, GUO Ling, YANG Shao-lin, WEN Cai-fang, ZHANG Man-li, LUO Lu, XI Li-xin, YANG Yan, CHEN Li-min, YAN Qi-gui. Isolation and Identification of K.pneumoniae in Takin[J]. , 2015, 42(1): 203-208.

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Isolation and Identification of K.pneumoniae in Takin

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