刚地弓形虫GRA25基因的克隆及序列分析

doi:10.16431/j.cnki.1671-7236.2016.03.013

Abstract

Abstract: In this study,sequence variation of GRA25 genes among 22 Toxoplasma gondii (T.gondii) strains from different hosts and geographical locations were examined.The complete GRA25 genes from 22 T.gondii isolates were amplified,sequenced,and nucleotide variations were determined.Phylogenetic analysis among the different T.gondii isolates were conducted using maximum parsimony (MP) and maximum likelihood (ML) methods.The biological characteristics of the protein GRA25 of the T.gondii RH strain were predicted using bioinformatics software.The sequences of all the examined T.gondii strains were 939 or 948 bp in length.Sequence comparison of all 22 GRA25 sequences identified 82 variable nucleotide positions (0 to 4.4%).The results of phylogenetic analysis showed that strains belonging to the classical typeⅠand Ⅱ could not group into their own branches based on the GRA25 sequences.Bioinformatics analysis revealed that the protein GRA25 contained 7 hydrophobicity regions,10 alpha regions,3 beta sheets,8 random coils and 8 linear B-cell epitopes.These results suggested that the GRA25 gene was not an ideal genetic marker for population genetic study of T.gondii strains,but it might represent a good vaccine candidate against toxoplasmosis.

Key words: Toxoplasma gondii; GRA25 gene; sequence analysis; bioinformatics analysis

CLC Number:

S852.7

XU Xiao-pei, LIU Wen-ge, ZHANG Nian-zhang, CHEN Jia, ZHOU Dong-hui, ZHU Xing-quan, XU Qian-ming. Cloning and Sequence Analysis of Toxoplasma gondii GRA25 Gene[J]. , 2016, 43(3): 650-655.

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Cloning and Sequence Analysis of Toxoplasma gondii GRA25 Gene

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