弓形虫细胞核因子3基因的克隆及序列分析

doi:10.16431/j.cnki.1671-7236.2015.02.005

Abstract

Abstract: In order to predict the possibility of Toxoplasma gondii nuclear factor 3 (TgNF3) as anti-Toxoplasma gondii vaccine candidate,TgNF3 gene was amplified by one step RT-PCR using a pair of specific primers.Then the fragments were cloned into the pMD18-T vector.The sequence of TgNF3 gene was then translated into amino acids and analyzed by bioinformatics software.The physical and chemical characteristics,hydrophilic domain,transmembrane domain,epitopes,and advanced structure of TgNF3 were predicted by multiple bioinformatics approaches.The results revealed that TgNF3 gene was approximate 950 bp in length.TgNF3 protein was predicted to contain only one transmembrane domain,9 α-helix regions,4 β-fold regions,8 hydrophilic regions and 10 flexible regions,and predicted to contain 9 linear B cell epitopes.The results indicated that TgNF3 could be a vaccine candidate antigen against Toxoplasma gondii,which provided the basic data for development of novel Toxoplasma gondii vaccines.

Key words: Toxoplasma gondii; Toxoplasma gondii nuclear factor 3 (TgNF3) gene; clone; bioinformatics analysis

CLC Number:

S852.7

HU Ling-ying, ZHANG Nian-zhang, GAO Qi, ZHU Xing-quan, WANG Shou-kun. Cloning and Sequence Analysis of Toxoplasma gondii NF3 Gene[J]. , 2015, 42(2): 270-274.

References

[1] Hill D E,Chirukandoth S,Dubey J P.Biology and epidemiology of Toxoplasma gondii in man and animals[J].Anim Health Res Rev,2005,6(1):41-61.
[2] Dubey J P.Toxoplasmosis of animals and humans[M].Boca Raton:CRC Press,2010.
[3] Gao X J,Zhao Z J,He Z H,et al.Toxoplasma gondii infection in pregnant women in China[J].Parasitology,2012,139(2):139-147.
[4] Sumi M,Aosai F,Norose K,et al.Acute exacerbation of Toxoplasma gondii infection after hematopoietic stem cell transplantation:Five case reports among 279 recipients[J].Int J Hematol,2013,98(2):214-222.
[5] Zhou P,Chen Z,Li H L,et al.Toxoplasma gondii infection in humans in China[J].Parasit Vectors,2011,4(1):165.
[6] Dubey J P.The history of Toxoplasma gondii-The first 100 year[J].Int J Parasitol,2008,55(6):467-475.
[7] Dubey J P.Toxoplasmosis in pigs-The last 20 years[J].Vet Parasitol,2009,164(2-4):89-103.
[8] Zhang N Z,Chen J,Wang M,et al.Vaccines against Toxoplasma gondii:New developments and perspectives[J].Expert Rev Vaccines,2013,12(11):1287-1299.
[9] Olguin-Lamas A,Madec E,Hovasse A,et al.A novel Toxoplasma gondii nuclear factor TgNF3 is a dynamic chromatin-associated component,modulator of nucleolar architecture and parasite virulence[J].PLoS Pathog,2011,7(3):e1001328.
[10] Zhang Y.I-TASSER server for protein 3D structure prediction[J].BMC Bioinformatics,2008,9:40.
[11] Zhang Y.I-TASSER:Fully automated protein structure prediction in CASP8[J].Proteins,2009,77(S9):100-113.
[12] Zhang N Z,Huang S Y,Zhou D H,et al.Identification and bioinformatics analysis of a putative calcium-dependent protein kinase (CDPK6) from Toxoplasma gondii[J].Genet Mol Res,13(4):10669-10677.
[13] Bai Y,He S,Zhao G,et al.Toxoplasma gondii:Bioinformatics analysis,cloning and expression of a novel protein TgIMP1[J].Exp Parasitol,2012,132(4):458-464.
[14] 高琦,张念章,胡玲英,等.弓形虫DOC2基因C-端的克隆及序列分析[J].中国畜牧兽医,2014,41(8):56-60.
[15] 徐颖,张念章,谭启东,等.弓形虫RH株ROP38基因的原核表达及其反应原性研究[J].中国兽医科学,2014,44(1):83-87.
[16] 陈锐钊,张念章,聂子雄,等.弓形虫致密颗粒蛋白14基因的克隆及序列分析[J].中国兽医科学,2013,43(3):238-242.

Cloning and Sequence Analysis of Toxoplasma gondii NF3 Gene

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