荷包猪SLA-DRa基因的克隆及序列分析

doi:10.16431/j.cnki.1671-7236.2015.02.008

Abstract

Abstract: In order to study SLA-DRa gene (also named as SLA-DRa-HB) from one of special breed of domestic pig,Hebao,primers were designed to amplify the coding domain of SLA-DRa from three Hebao pigs.Then,the amplified fragments were cloned into pMD18-T vector followed by transforming them into Escherichia coli JM109.After cleaving by the restricted enzymes,the positive clones were selected to be sequenced.The differences between SLA-DRa-HB and other SLA-DRa alleles were analyzed by sequences comparing,and then the phylogenetic tree was constructed.The results showed that the interest of the fragments was successfully amplified by RT-PCR and the molecular weight was about 800 bp.By cloning and sequencing,the whole length of SLA-DRa-HB was 779 bp and the open reading fragments (ORF) of SLA-DRa-HB located at sites of 1 to 759 coding for 252 amino acids.Results of sequences comparision and analysis showed that the characterized amino acids of SLA-DRa-HB mainly focused on sites of 135,159 and 202,while the mutational sites in trans-membrane and cytoplasm were 206 and 248.Analyzing from the phylogenetic tree,it showed that SLA-DRa-HB was clustered into one branch and they were relatively closed to other SLA-DRa alleles.In this research,the SLA-DRa alleles were cloned successfully from Hebao pigs and it would lay a base for study the function of the SLA-DRa genes.

Key words: Hebao pigs; SLA-DRa gene; clone; sequence analysis

CLC Number:

Q786

JIANG Ping, GAO Feng-shan, Erdemtu. Cloning and Sequences Analysis of the SLA-DRa Gene from Hebao Pig[J]. , 2015, 42(2): 264-269.

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Cloning and Sequences Analysis of the SLA-DRa Gene from Hebao Pig

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