陆川猪磷酸酪氨酸互作结构域1基因克隆及序列分析

doi:10.16431/j.cnki.1671-7236.2015.02.003

Abstract

Abstract: In order to clone the phosphotyrosine interaction domain containing 1(PID1) gene of Luchuan pig,a pair of special primers was designed according to released sequence of Laiwu pig PID1 gene in GenBank.The PID1 gene was amplified by RT-PCR,its gene sequence characteristics and protein structure was systemically analyzed by bioinformatics techniques.The results showed that the cloned PID1 gene fragment included a 654 bp whole length CDS (coding 217 amino acids).The sequence multi-aligned results showed that Luchuan pig shared 99.08%,87.61%,93.88%,93.58%,90.06%,83.79%,66.94%,66.52% and 60.09% of similar nucleotide sequence with that of Laiwu pig,Bos,Macaca,Homos,Mus,Gallus,Rattus,Danio retio and Xenopus, respectively.The phylogenetic tree indicated that PID1 gene was highly conserved in the process of evolution of different species.The cloning and analysis of PID1 gene provided an important foundation for further study regulation mechanism of PID1 gene in Luchuan pig.

Key words: Luchuan pig; PID1 gene; cloning

CLC Number:

Q785

XIE Bing-kun, QIN Zhao-xian, WU Yong-shao, NONG Su-qun, LAN Hai-en, LV Ling-yan, PAN Tian-biao. Cloning and Sequence Analysis of PID1 Gene in Luchuan Pig[J]. , 2015, 42(2): 258-263.

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Cloning and Sequence Analysis of PID1 Gene in Luchuan Pig

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