雷公山山区放牧山羊黑素皮质素受体-4 mRNA表达的研究

doi:10.16431/j.cnki.1671-7236.2016.03.006

Abstract

Abstract: In order to understand the differential expression among tissues or breeds of grazed goat,β-actin gene was used as control,the expression levels of melanocortin-4 receptor (MC4R) mRNA in 7 tissues of Qiandongnan small Xiang goat,Guizhou White goat,Guizhou Black goat,Qianbei Ma goat and Nanjiang Yellow goat were detected by Real-time fluorescence quantitative PCR technique with double standard curve method.The results showed that MC4R mRNA was expressed in all 7 tissues of 5 grazed goat breeds,the expression levels of subcutaneous fat and kidney were the highest,the expression levels of liver was the second,the expression levels of lung and heart were the third,and the expression levels of semimembranosus muscle and longissimus muscle were the lowest.There were no differences of the expression level of MC4R mRNA in liver among different breeds.In subcutaneous fat,kidney,lung,heart,semimembranosus muscle and longissimus muscle,the highest expression levels of MC4R mRNA were all observed in Guizhou White goat.The results suggested that for grazed goats,the expression levels of MC4R mRNA existed tissue differences,and except for the liver,it existed breed differences,too.This research laid a basis for molecular marker assisted selection of growth traits,carcass traits,and meat quality traits of grazed goat.

Key words: grazed goat; melanocortin-4 receptor; gene expression

CLC Number:

S814

YANG Jia-da, WU Sheng-rong, PAN Sheng-rong. Study on Melanocortin-4 Receptor mRNA Expression in Goats Grazed in Leigongshan Mountain Area[J]. , 2016, 43(3): 601-607.

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Study on Melanocortin-4 Receptor mRNA Expression in Goats Grazed in Leigongshan Mountain Area

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