乌珠穆沁羊生长分化因子11基因外显子1序列CpG位点的甲基化模式分析

doi:10.16431/j.cnki.1671-7236.2016.02.007

Abstract

Abstract: In order to investigate the methylation patterns on growth differentiation factor 11 (GDF11) gene exon 1 in Ujumqin sheep,we compared the level of methylation of GDF11 gene exon 1 in common Ujumqin sheep (CUS) and multi-vertebrae Ujumqin sheep (MUS) using the bisulfite sequencing PCR (BSP) method.The results showed that the average methylation rate of GDF11 gene exon 1 for MUS was extremely significantly higher than that for CUS (P<0.01),the average methylation rate of common Ujumqin sheep and multi-vertebrae Ujumqin sheep were 0.123 and 0.569.Analysis of 13 CpGs of GDF11 gene exon 1 showed that the CpG_11 and CpG_13 methylation sites had the highest methylation rate with a combined methylation value of 90% for MUS.Our results suggested that differential DNA methylation of GDF11 gene exon 1,in particular at these two sites,might cause the increase in the number of vertebrae in Ujumqin sheep.

Key words: Ujumqin sheep; GDF11 gene; CpG site; methylation; bisulfite sequencing

CLC Number:

S826.8⁺2

HE Xiao-long, LI Bei, FU Shao-yin, LIU Yong-bin, QI Yun-xia, WANG Biao, TERI Gele, RONG Wei-heng, DA Lai. Analysis of the CpG Methylation Pattern on GDF11 Gene Exon 1 in Ujumqin Sheep[J]. , 2016, 43(2): 333-339.

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Analysis of the CpG Methylation Pattern on GDF11 Gene Exon 1 in Ujumqin Sheep

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