哺乳动物体内、外生产胚胎的表观遗传差异研究进展

doi:10.16431/j.cnki.1671-7236.2019.01.022

Abstract

Abstract:

As an efficient assisted reproductive technology,in vitro embryo production is of great significance to the conservation and effective utilization of germplasm resources and genetic improvement.However,compared with embryos produced in vivo,the embryos produced in vitro develop slowly,the cleavage rate is low and the rate of apoptosis increases in the process of culture,which are accompanied by placental hypertrophy and prolonged pregnancy after transplantation.Preterm delivery,low birth weight,and macrosomia syndrome (LOS) occur after birth which are closely related to abnormal gene expression and epigenetic modification.The progress of epigenetic modification in the production of embryos in vivo and in vitro was briefly reviewed in this paper.The differences of epigenetic modification of imprinted genes,DNA methylation and histone acetylation between mammalian embryos in vivo and in vitro were introduced.The application of microarray technique in the production of embryos in vitro and in vivo was briefly described in order to obtain the key imprinted genes that lead to the difference of embryo quality between in vivo and in vitro,and explored the reasons why the quality of embryos produced in vitro was lower than that of in vivo,and then improved the in vitro embryo production system.

Key words: blastocyst; methylation; epigenetics; histone acetylation

CLC Number:

S813.1

ZHAO Yahan, HAO Haisheng, DU Weihua, YAN Changliang, LIU Yan, PANG Yunwei, ZHU Huabin, ZHAO Xueming. Research Progress on Epigenetic Differences Between Mammalian Embryos Produced in vivo and in vitro[J]. , 2019, 46(1): 194-199.

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Research Progress on Epigenetic Differences Between Mammalian Embryos Produced in vivo and in vitro

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