Abstract: The study was aimed to rapidly diagnose two ill and dead cattle in three bay town of Yanji city in Jilin province.We collected liver,spleen,heart,muscle and other tissues to diagnose Clostridium chauvoei infection by epidemiological investigation,clinical autopsy,microscopic obsevation,bacterial culture observation,biochemical experiment,molecular biology diagnosis and animal experiment.The muscles of the ill and dead cattle contacted with crepitus,and the section had a lot of blood and bubble flowing out,microscopic obsevation found bacillus with obtuse at both ends and spores,the bottom of the tube had loose and white precipitate in the broth,biochemical experiments showed that the isolate had acid-production and gas-production reactions which was unique to anaerobic bacteria causing Clostridium chauvoei infection,and 501 bp fragment was amplified by PCR.The results showed that two ill and dead cattle were casued by Clostridium chauvoei infection in Yanbian area,and proved the existence of Clostridium chauvoei.The test separated Clostridium chauvoei Yanbian strain for the frist time,which provided important reference basis for the prevention and treatment of Clostridium chauvoei infection,and laid the important foundation for the further study of drug and immune preventions of this disease.

Key words: Clostridium chauvoei infection; autopsy; diagnosis