载脂蛋白CⅡ基因在猪肝脏组织中的发育性表达研究

doi:10.16431/j.cnki.1671-7236.2015.10.032

Abstract

Abstract: The aim of this study was to investigate the developmental patterns of ApoCⅡ gene mRNA in liver in Mashen and Large White pigs, and study the relationship between the expression level of ApoCⅡ and the lipid metabolism in pigs.The mRNA relative expressions of ApoCⅡ gene in liver at seven stages of 1,30,60,90,120,150,and 180-day old in Mashen and Large White pigs were determined by quantitative Real-time PCR.The results showed that the developmental trend of ApoCⅡ mRNA expression in liver between Mashen and Large White pigs was different.The ApoCⅡ mRNA abundance was decreased from birth to 60-day old,then increased at 90-day old,and decreased again after that in Mashen pig.However,the relative expression amount in Large White pig was gradually decreased from birth to 150-day old and increased again at 180-day old.Except for the ApoCⅡ mRNA expression amount at 1-day old,the differences of the expression amount at other stages in Mashen and Large White pigs were significant or extremely significant (P<0.05 or P<0.01).The ApoCⅡ mRNA expression in liver was affected by age and breed,and could play an important role in lipid metabolism in pigs.

Key words: pig; ApoCⅡ gene; developmental expression; liver

CLC Number:

S813.3

LIU Cui-cui, GUO Xiao-hong, CAO Guo-qing, GAO Peng-fei, PU Zhong-de, NIU Jiao-yan, ZHANG Qi, LI Meng, JIN Yu-shu, LI Bu-gao. Study on Developmental Expression of ApoCⅡ Gene mRNA in Liver Tissues of Pigs[J]. , 2015, 42(10): 2726-2731.

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Study on Developmental Expression of ApoCⅡ Gene mRNA in Liver Tissues of Pigs

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