胎衣不下奶牛母体胎盘α-烯醇化酶异常表达分析及验证

doi:10.16431/j.cnki.1671-7236.2015.10.030

Abstract

Abstract: The paper aimed to analyze and validate the function of differentially expressed ENO1 in the cow with retained foetal membrane.In our research,we chose three healthy Holstein dairy cows and three Holstein dairy cows with retained foetal membrane of similar age,foetal times,weight and milk yield and divided them into two groups.The total protein of maternal placenta was extracted in the control and retained foetal membrane of cow.The differential expression of proteins were found out by the 2-DIGE,and the differential expression of ENO1 was validated by the Western blotting and Real-time quantitative PCR.The results showed that ENO1 was significant expression and large multiple in the two groups,and it was significant increased expression in the retained foetal membrane of cow after Western blotting and Real-time quantitative PCR (P=0.015<0.05;P=0.001<0.01).The ENO1 participated in the energy homeostasis,immune and fibrinolysis process which related to the retained foetal membrane of cow.It suggested that ENO1 was likely connected with the retained foetal membrane.

Key words: retained foetal membranes; ENO1; Western blotting; Real-time quantitative PCR

CLC Number:

S823.9⁺1

ZOU Xiao, LIU Yao, ZHENG Cheng-yuan, ZHANG Lu-ying, XU Qing-you, LI Shi-ying, LUO Chun-hai, LIU Jian-ying, FU Shi-xin. Analysis and Validation of the Differentially Expressed ENO1 in the Maternal Placenta of Cow with Retained Foetal Membrane[J]. , 2015, 42(10): 2714-2719.

References

[1] 黄光红.奶牛胎衣不下的不同治疗方法对其生产和繁殖性能的影响[J].上海畜牧兽医通讯,2013,4:50-51.
[2] 陈学文,李旭,凌泽辉,等.广西荷斯坦奶牛胎衣不下的病因、发病规律及防制措施[J].中国畜牧兽医,2009,36(5):130-133.
[3] McNaughton A P,Murray R D.Structure and function of the bovine fetomaternal unit in relation to the causes of retained fetal membranes[J].Vet Rec,2009,165:615-622.
[4] 曹学法,赵国荣.奶牛胎衣不下的病因分析[J].养殖与饲料,2014,10:52-54.
[5] Ejeskar K,Krona C,Caren H,et al.Introduction of in vitro transcribed ENO1 mRNA into neuroblastoma cell death[J].BMC Cancer,2005,5:161-174.
[6] 刘养奇,邢军明,李戈楠,等.α-烯醇化酶与消化系统肿瘤的研究进展[J].现代肿瘤医学,2014,22(11):2738-2740.
[7] 朱理安,方宁远.α-烯醇化酶——古老的蛋白,崭新的功能[J].国际病理学与临床杂志,2007,4:347-350.
[8] Díaz-Ramos A,Roig-Borrellas A,García-Melero A,et al.α-enolase,a multifunctional protein:Its role on pathophysiological situations[J].Biomed Biotechnol,2012,2012:156795.
[9] Alam J,Kim Y C,Choi Y.Potential role of bacterial infection in autoimmune diseases:A new aspect of molecular mimicry[J]. Immune Netw,2014,14(1):7-13.
[10] Song Y,Luo Q,Long H,et al.Alpha-enolase as a potential cancer prognostic marker promotes cell growth,migration,and invasion in glioma[J].Mol Cancer,2014,13:65.
[11] 高玒,余传信.烯醇化酶与寄生虫感染[J].中国血吸虫病防治杂志,2014,26(4):445-448.
[12] Oliveira D M,Chalfun-Junior A,Chizzotti M L,et al.Expression of genes involved in lipid metabolism in the muscle of beef cattle fed soybean or rumen-protected fat,with or without monensin supplementation[J].J Anim Sci,2014,92(12):5426-5436.
[13] 聂甜甜,郑程远,邹晓,等.胎衣不下奶牛胎盘组织差异表达蛋白的筛选及鉴定[J].中国生物制品学杂志,2015,28(1):54-57.
[14] 周帮会,翟向河,陈斌,等.奶牛发生胎衣不下的胎盘免疫学及中药防治机理[J].江苏农业科学,2013,41(10):181-183.
[15] 周帮会,刘荣欣,姜国均,等.益母生化散对胎衣不下奶牛胎盘激素含量的影响[J].中国兽医学报,2010,30(7):988-991.
[16] 王建永,周帮会,李海燕,等.胎衣不下奶牛与健康奶牛胎盘中前列腺素和血管紧张素含量的比较[J].安徽农业大学学报,2010,37(3):449-453.
[17] Nau P N,Natta T V,Ralphe J C,et al.Metabolic adaptation of the fetal and postnatal ovine heart:Regulatory role of hypoxia-inducible factors and nuclear respiratory factor-1[J].Pediatric Res,2002,52(2):269-278.
[18] Snir O,Widhe M,Hermansson M,et al.Antibodies to several citrullinated antigens are enriched in the joints of rheumatoid arthritis patients[J].Arthritis and Rheumatism,2010,62(1):44-52.
[19] Wygrecka M,Marsh L M,Morty R E,et al.Enolase-1 promotes plasminogen-mediated recruitment of monocytes to the acutely inflamed lung[J].Blood,2009,113(22):5588-5598.
[20] Takagi M,Yamamoto D,Ohtani M,et al.Quantitative analysis of messenger RNA expression of matrix metalloproteinases (MMP-2 and MMP-9),tissue inhibitor-2 of matrix metalloproteinases (TIMP-2),and steroidogenic enzymes in bovine placentomes during gestation and postpartum[J].Mol Reprod Dev,2007,74(7):801-807.

Analysis and Validation of the Differentially Expressed ENO1 in the Maternal Placenta of Cow with Retained Foetal Membrane

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LYU Xiaonan, XU Lipu, ZHANG Wen, WANG Na, CAO Huan, WANG Xiaoliang, WANG Shu, WANG Jingbo. Research on Histopathological Changes and Virus Distribution Situation of Mirror Carps Infected with Carp Edema Virus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1077-1084.
[2]	ZHANG Junfang, SUN Jianfu, SUN Bin, TANG Lin, WANG Ying, WANG Enze, CUI Yan, LI Qiang, YAN Changguo, LI Xiangzi. Cloning and Bioinformatics Analysis of PPARγ Gene of Yanbian Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2695-2704.
[3]	MIAO Manning, GUO Yiwen, HU Debao, ZENG Yuhan, LI Xin, ZHANG Linlin, DING Xiangbin, GUO Hong. Transcriptome Sequencing and Bioinformatics Analysis of MSTN Gene-edited Bovine Muscles [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2271-2281.
[4]	LI Heng, ZI Xiangdong. Cloning and Expression in Cumulus-oocyte Complexes of Yak TEDDM1 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1284-1293.
[5]	CHEN Lei, YUAN Feng, HE Sangang, MAYILA, LI Wenrong. Cloning,Sequencing and Quantitative Expression of FGF10 Gene in Sheep During Hair Follicle Development Period [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(2): 425-432.
[6]	DAI Zhenglie, ZHU Jingjing, CHEN Zhenyu, ZHOU Xiaolong, WANG Han, LI Xiangchen, ZHAO Ayong, YANG Songbai. Cloning, Bioinformatics Analysis and Expression of Porcine SENP1 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(12): 4382-4390.
[7]	JIANG Yixuan, WANG Yaling, XING Shanshan, SONG Guohua, XU Huifen, LI Ming. Cloning, Bioinformatics Analysis and Expression Profile Construction of MSTN Gene in New Zealand White Rabbits [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3501-3511.
[8]	LI Zhiqiang, TAO Chenyu, WEI Aolong, JIA Qing. Establish on Real-time Quantitative PCR Method for Purity Detection of Porcine Sex-controlled Sperm [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3682-3690.
[9]	WANG Zhengfan, ZHU Lisai, WANG Juan, LI Yiyun, XIANG Rui, YING Biyun, WANG Guiping, JIA Aiqing, BAI Aiquan. Establishment and Preliminary Application of Duplex Real-time Quantitative PCR for Detection of PDCoV and TGEV [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3855-3863.
[10]	LI Anqi, JIANG Lei, SU Xiaotong, ZAN Linsen, WANG Hongbao. Expression Characteristics Analysis of ACTC1 Gene in Qinchuan Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2359-2367.
[11]	LI Jinghui, XING Siyuan, WANG Xicai, LI Qinghe, ZHAO Guiping, ZHANG Yonghong, WEN Jie, LIU Ranran. Screening of Candidate Genes Related to Intramuscular Fat Deposition in Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1828-1836.
[12]	KONG Zimeng, JIANG Bo, CAI Yunhong, HE Houjun, LI Yongqing, JIN Huan. Cloning,Protein Expression and Purification of Chicken Complement Receptor 2 Gene and Preparation and Identification of Its Polyclonal Antibody [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(1): 201-208.
[13]	ZHANG Ji, YI Ming, CHENG Chaoyou, CHENG Junhua, FU Zhengxian, YANG Dewen, WU Xue, QIU Ganyuan, LIU Ruoyu. Study on Tissue Expression of SOCS7 and GFAP Genes in Weining Sheep [J]. , 2019, 46(7): 1899-1906.
[14]	ZHAO Chengxiang, DAI Kaiyu, DU Yulu, BAO Wenbin, WU Shenglong. Analysis of the Relationship Between Expression Level of Porcine TLR5 Gene and Infection of Escherichia coli F18 [J]. , 2018, 45(5): 1163-1169.
[15]	HUA Min, HE Xinwei, WAN Run, CHENG Zhentao, ZHOU Bijun, WEN Ming. Sequence Analysis of Cofilin2 Gene and Its Expression in Different Tissues of Sansui Ducks [J]. , 2018, 45(4): 858-865.