山羊碱性氨基酸转运载体基因SLC3A1cDNA序列克隆及表达

doi:10.16431/j.cnki.1671-7236.2015.10.001

Abstract

Abstract: The objective of this study was to clone caprine cationic amino acid transporter gene SLC3A1 and investigate its mRNA expression in different tissues and its development regularity in small intestine.The cDNA sequence of caprine SLC3A1 gene was cloned with the primer which was designed according to mRNA sequence of Ovis aries and Bos taurus in GenBank.Then its expression was quantified by Real-time PCR in 11 tissues from goats at the age of day 1 and duodenum,jejunum and ileum from goats at the age of day 1,month 6,month 8,month 10 and month 12.The results indicated that cDNA sequence of SLC3A1 gene was obtained,and its homology with SLC3A1 gene in Ovis aries,Bos taurus,Sus scrofa,Homo sapiens,Mus musculus and Rattus norvegicus were 99%,97%,88%,86%,80%,79%,respectively.SLC3A1 gene was expressed in all of these collected tissues,whereas the expression level varied from tissues.Specifically,its expression in kidney,jejunum,ileum and colon were significant higher than that in other tissues at the age of day 1 and decreased systematically (P<0.05).The expression of SLC3A1 in small intestine at the same age of goat was ileum>jejunum>duodenum.SLC3A1 gene expression in duodenum at the age of day 1 was significant higher than that at the other ages (P<0.05),it decreased with age in jejunum,and there was no significant difference among ileum with age (P>0.05). In conclusion,SLC3A1 gene and b^0,+ cationic amino acid transporter system were mainly expressed in kidney and intestine.The expression of SLC3A1 gene was differentially regulated and distributed by developmental stages and segments of small intestine in goat.

Key words: goat; SLC3A1 gene; cDNA; Real-time PCR; mRNA expression

ZHOU Ying-hao, GAO Ye, LIU Lin-li, ZENG Jie, YANG Yu-xin, ZHANG En-ping, CHEN Yu-lin. Cloning and Expression of Caprine Cationic Amino Acid Transporter Gene SLC3A1 cDNA Sequence[J]. , 2015, 42(10): 2513-2520.

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Cloning and Expression of Caprine Cationic Amino Acid Transporter Gene SLC3A1 cDNA Sequence

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