长链非编码RNA(H19)在小鼠骨骼肌生长发育过程中的表达

doi:10.16431/j.cnki.1671-7236.2015.10.002

Abstract

Abstract: This experiment was intend to study the changes of long non-coding RNA (H19) expression levels in skeletal muscle development and regeneration,and lay the foundation of its mechanism reach in skeletal muscle development.C2C12 cell line and ICR mice were used as experimental material,bioinformatics assay was used to exploit its non-coding character and low conservatism in different species,and the expressions of H19 in C2C12 cell differentiation,skeletal muscle development and the phase of muscle regeneration were detected by quantitative Real-time PCR (qRT-PCR).The results showed that,H19 expression levels in postnatal mouse skeletal muscle decreased with increasing age;during C2C12 cell differentiation,H19 mRNA increased gradually,and then maintained a high level;The expression of H19 was maintained at a high level through days 4 to 6 after injury.In consideration of its express character in C2C12 cell differentiation and skeletal muscle damage repair model,H19 may play an important role in promoting myogenesis and skeletal muscle regeneration.

Key words: H19; mouse; skeletal muscle; muscle regeneration

CLC Number:

S813.24

WANG Zi-shuai, HUA Chao-ju, WANG Chen, LI Kui, TANG Zhong-lin. The Expression of Long Noncoding RNA (H19) in Mouse Skeletal Muscle Development[J]. , 2015, 42(10): 2521-2528.

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The Expression of Long Noncoding RNA (H19) in Mouse Skeletal Muscle Development

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