奶牛乳腺上皮细胞原代培养、纯化及鉴定技术研究进展

doi:10.16431/j.cnki.1671-7236.2015.08.027

Abstract

Abstract: The bovine mammary epithelial cells not only have the function of synthesis and secretion of milk, but also play an important role in innate immunity system of the mammary gland, and there is great significance of them on studying the mechanism of lactation, mastitis pathogenesis and drug screening.Primary cultured bovine mammary gland epithelial cells are suitable for setting up cell model which can be used as the dielectric for physiological, pathological and pharmacological researching, avoiding the difficulties of in vivo test, such as the long cycle, the high cost, the individual difference, etc.The author summarized the latest researches of cell primary culture in vitro, cultivation technology, purification and identification method in order to provide reference for the studies of bovine mammary epithelial cells culture.

Key words: bovine; mammary epithelial cells; primary culture; identification

CLC Number:

S823

LIN Jie, WANG Xu-rong, LI Jian-xi, WANG Xiao-wu, YANG Zhi-qiang. Research Progress on Primary Culture,Purification and Identification Technique of Bovine Mammary Epithelial Cells[J]. , 2015, 42(8): 2109-2115.

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Research Progress on Primary Culture,Purification and Identification Technique of Bovine Mammary Epithelial Cells

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