›› 2015, Vol. 42 ›› Issue (7): 1793-1799.doi: 10.16431/j.cnki.1671-7236.2015.07.025

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Association Study on PPARD Gene G32E Polymorphic Site with Fat Deposit Related Traits in Beijing Black Pigs

LIANG Jing1, WANG Hong-wei2, CHENG Li-an2, ZHANG Long-chao1, WANG Li-gang1, LIU Xin1, SHI Guo-hua2, YANG Hong-ping2, LIU Xian-ce2, SHI Hui-bi1, ZHANG Yue-bo1, ZHANG Tian1, WANG Li-xian1   

  1. 1. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    2. Beijing Heiliu Animal Husbandry Science and Technology Co., Ltd., Beijing 102211, China
  • Received:2014-12-23 Online:2015-07-20 Published:2015-07-23

Abstract: The objective of this study was to analyze the association of a missense mutation G32E in the first exon of peroxisome proliferator-activated receptor delta (PPARD) gene with fat deposit related traits in Beijing Black pigs.Detected the genotype at G32E site in Beijing Black pig population and several Chinese and foreign pig populations using Sequenom mass spectrometry,and analyzed polymorphism with backfat thickness and IMF in Beijing Black pig population.The results indicated that backfat thickness of individuals with genotype GG was extremely significant higher than those individuals with genotypes GA and AA (P<0.01) in Beijing Black pig population,but no significant different of IMF was found (P>0.05).Allele A was high in Chinese local pigs Meishan and Erhualian.But in Minzhu,Laiwu,Yacha,and foreign commercial pigs Duroc,Landrace,Large White,mainly was allele G.The result suggested that polymorphism of PPARD gene G32E site had significant impact on backfat thickness,and no effect on IMF trait.

Key words: pig; PPARD gene; DNA polymorphism; backfat thickness

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