Pax7基因在猪不同组织中的表达特征及其在背最长肌中的发育性表达规律研究

doi:10.16431/j.cnki.1671-7236.2015.06.027

Abstract

Abstract: The aim of this study was to investigate the expression profile of Pax7 (paired box 7) gene in different tissues and its developmental expression in longissimus dorsi of pigs.The expression profiles of Pax7 gene at the levels of mRNA and protein in 12 different tissues including heart,liver,spleen,lung,kidney,stomach,small intestine,hypothalamus,cerebellum,longissimus dorsi,biceeps femoris,psoas major of Mashen pigs at birth and the developmental expression patterns in longissimus dorsi at seven stages (1,30,60,90,120,150,and 180-day old) of Mashen and Large White pigs were studied by quantitative Real-time PCR and Western blotting.The results showed that the Pax7 mRNA was expressed in longissimus dorsi,biceeps femoris,psoas major,hypothalamus,and cerebellum,wherease the Pax7 protein was only expressed in skeletal muscles of longissimus dorsi,biceeps femoris,and psoas major.The developmental expression patterns of Pax7 mRNA and protein in Mashen pig was basically in accordance with those in Large White pig.The mRNA expression amount of Pax7 gene in longissimus dorsi was the greatest at 30-day old (P <0.01),followed by the expression at 1-day old,and kept lower stable levels at other stages both in Mashen and Large White pigs.At the stages of 30-day,90-day,and 180-day old,Pax7 mRNA expression amounts in Mashen pig were significantly higher than those in Large White pig (P <0.01).The Pax7 protein expression at 1-day old was the greatest (P <0.01),followed by it at 30-day old,which were significantly greater than those at other stages,at which Pax7 expression was in lower stable levels.The Pax7 protein expression from one-day old to 180-day old in Mashen pig was significantly greater than those in Large White pig (P <0.05;P <0.01).The expression of Pax7 gene could associated with tissue,age,and background of pig breed.

Key words: pig; Pax7 gene; expression profile; developmental expression

CLC Number:

S828.2

NIU Jiao-yan, GAO Peng-fei, MA Li, LIU Cui-cui, WANG Ze-yi, YANG Qing-chun, PU Zhong-de, ZHANG Qi, LIU Hong, GUO Xiao-hong, LI Bu-gao, CAO Guo-qing. Study on the Expression Profile of Pax7 Gene and its Developmental Expression in Longissimus Dorsi of Pigs[J]. , 2015, 42(6): 1504-1511.

References

[1] Lang D,Powell S K,Plummer R S,et al.PAX genes:Roles in development,pathophysiology,and cancer[J].Biochem Pharmacol,2007,73(1):10-14.

[2] Jostes B,Walther C,Gruss P.The murine paired box gene,Pax7,is expressed specifically during the development of the nervous and muscular system[J].Mech Develop,1990,33(1):27-37.

[3] Peters H,Wilm B,Sakai N,et al.Pax1 and Pax9 synergistically regulate vertebral column development[J]. Development,1999,126(23):5399-5408.

[4] Ziman M R,Thomas M,Jacobsen P,et al.A key role for Pax7 transcripts in determination of muscle and nerve cells[J].Exp Cell Res,2001,268(2):220-229.

[5] Mansouri A,Gruss P.Pax3 and Pax7 are expressed in commissural neurons and restrict ventral neuronal identity in the spinal cord[J]. Mech Develop,1998,78(1):171-178.

[6] Cai L,Taylor J F,Wing R A,et al.Construction and characterization of a bovine bacterial artificial chromosome library[J]. Genomics,1995,29(2):413-425.

[7] Sibthorpe D,Sturlaugsdottir R,Kristjansson B K,et al.Characterisation and expression of the paired box protein 7 (Pax7) gene in polymorphic Arctic charr (Salvelinus alpinus)[J]. Comp Biochem Phys B,2006,145(3-4):371-383.

[8] James E N M,Simon M H.Sequential actions of Pax3 and Pax7 drive xanthophore development in zebrafish neural crest[J]. Dev Biol,2008,317(2):508-522.

[9] Thomas M G,Barker R A,Beazley L D,et al.Pax7 expression in the adult rat superior colliculus following optic nerve injury[J]. Neuroreport,2007,18(2):105-109.

[10] Hyatt J P K,McCall G E,Kander E M,et al.PAX3/7 expression coincides with MyoD during chronic skeletal muscle overload[J].Muscle Nerve,2008,38(1):861-866.

[11] Seger C,Hargrave M,Wang X,et al.Analysis of Pax7 expressing myogenic cells in zebrafish muscle development,injury,and models of disease[J].Dev Dynam,2011,240(11):2440-2451.

[12] Bhagavati S,Song X,Siddiqui M A Q.RNAi inhibition of Pax3/7 expression leads to markedly decreased expression of muscle determination genes[J].Mol Cell Biochem,2007,302(1-2):257-262.

[13] Galli L M,Knight S R,Barnes T L,et al.Identification and characterization of subpopulations of Pax3 and Pax7 expressing cells in developing chick somites and limb buds[J]. Dev Dynam,2008,237(7):1862-1874.

[14] Seale P,Rudnicki M A.A new look at the origin,function,and "stem-cell" status of musclesatellite cells[J].Dev Biol,2000,218(2):115-124.

[15] Buckingham M,Rigby P W J.Gene regulatory networks and transcriptional mechanisms that control myogenesis[J].Dev Cell, 2014,28(3):225-238.

[16] Maltzahn J V,Jones A E,Parks R J,et al.Pax7 is critical for the normal function of satellite cells in adult skeletal muscle[J].Proc Natl Acad Sci USA,2013,110(41):14674-14679.

[17] 郑江,邓忠良.Pax7对肌肉形成作用的研究进展[J].重庆医科大学学报,2007,32(6):667-669.

[18] Oustanina S,Hause G,Braun T.Pax7 directs postnatal renewal and propagation of myogenic satellite cells but not their specification[J].Embo,2004,23(16):3430-3439.

[19] Gotensparre S M,Andersson E,Wargelius A,et al.Insight into the complex genetic network of tetraploid Atlantic salmon (Salmo salar L.):Description of multiple novel Pax-7 splice variants[J]. Gene,2006,373:8-15.

[20] 甘甜,冷向军,郭婷,等.草鱼Pax7基因的克隆、序列分析及组织表达[J].生物技术通报,2014,11:152-158.

[21] 陈禧.鸭Pax3/7 cDNA序列克隆及表达特性研究[D].雅安:四川农业大学,2012.

[22] Chen J,Wang D Z.microRNAs in cardiovascular development[J].Mol Cell Cardiol,2012,52:949-957.

[23] 吕萍,马景涛.骨骼肌卫星细胞的分离及培养[J].河北医科大学学报,2006,27(4):305-307.

[24] 甘乾福.牛肌肉生长和脂肪代谢相关转录因子的克隆、表达及遗传效应分析[D].杨凌:西北农林科技大学,2009.

[25] Patruno M,Caliaro F,Maccatrozzo L,et al.Myostatin shows a specific expression pattern in pig skeletal and extraocular muscles during pre and post-natal growth[J].Differention,2008,76(2):168-181.

[26] Ropka M K,Eckert R,Piorkowska K.The expression pattern of myogenic regulatory factors MyoD,Myf6 and Pax7 in postnatal porcine skeletal muscles[J]. Gene Expr Patterns,2011,11(1-2):79-83.

[27] Yin H D,Li D Y,Zhang M Y,et al.Housing system influences abundance of Pax3 and Pax7 in postnatal chicken skeletal muscles[J].Poult Sci,2014,93(6):1337-1343.

[28] Qin W,Bruce M P.Regulation of MyoD function in the dividing myoblast[J].Febs Lett,2001,490(3):171-178.

[29] 赵晓,莫德林,张悦,等.猪的骨骼肌生长发育研究进展[J].生命科学,2011,23(1):37-44.

[30] Zhao P,Hofman E P.Embryonic myogenesis pathways in muscle regeneration[J].Dev Dyn,2004,229(2):380-392.

[31] 贾静敏.马身猪和大白猪肌球蛋白重链(MyHC)的发育性变化及肌纤维组织学观察[D].太谷:山西农业大学,2012.

Study on the Expression Profile of Pax7 Gene and its Developmental Expression in Longissimus Dorsi of Pigs

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WEI Mingbang, BIANBA Qiongda, XIAO Qingqing, DUAN Mengqi, CHAMBA Yangzom, SHANG Peng. Cloning,Bioinformatics and Expression Analysis of CDKN1B Gene in Tibetan Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2196-2206.
[2]	ZHANG Fangwei, ZHANG Qi, LEI Liangliang, SUN Wusheng, ZHANG Di, ZHANG Yunpeng, ZHANG Jingbo, WANG Xiuquan, ZHANG Jing, ZHANG Shumin. Polymorphism of HBEGF Gene and Its Association with Reproductive Traits in Songliao Black Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2370-2379.
[3]	PAN Pengcheng, LEI Zongquan, LI Xian, HU Xiangyun, QIN Qiantao, QIN Zhaoxian, GUAN Zhihui, CHEN Baojian, XIE Bingkun. Bioinformatics Analysis,Eukaryotic Expression Vector Construction and Tissue Expression of MYL2 Gene in Luchuan Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1796-1806.
[4]	OUYANG Kun, WEI Limin, SUN Ruiping, XIE Yali, QI Qi, LI Xiang, LIU Quanwei, CHAO Zhe, ZHANG Yongliang, CHEN Ting. Effects of Curcumin on Growth Performance,Feed Apparent Digestibility and Serum Biochemical Indexes in Hainan Tunchang Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1867-1875.
[5]	WU Jingxuan, SUN Zheng, MA Xuelian, ZHANG Liyang, LIAO Xiudong, LUO Xugang, LI Sufen, LYU Lin. A Survey on Magnesium Contents in Feed Resources for Livestock and Poultry in China [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1888-1898.
[6]	WANG Zeping, WANG Feifei, ZHANG Ruiming, FU Yanfeng, ZHAO Weimin, DAI Chaohui, CHENG Jinhua, LIAO Chao, LI Hui. Analysis on Polymorphism of SLA-1 Gene and Its Disease Resistance Potential in Suzi Pig [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1918-1927.
[7]	LIU Hongrun, ZHU Siran, FENG Lingli, ZHANG Kun, YAN Gang, WANG Yubin, ZHANG Shuai, JIANG Shan, XU Di, LAN Ganqiu, LIANG Jing. Cloning,Bioinformatics Analysis and Tissue Expression Localization of CDO1 Gene in Large White Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1352-1363.
[8]	YAO Kang, CAO Shuting, JIANG Zongyong, WANG Li. The Research Progress of Lysine Application and Requirement in Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1364-1374.
[9]	ZHANG Rui, MU Chunyu, TANG Qingping, BU Zhu, YANG Mingjun, XIA Aiping, YANG Xiaoming. Polymorphism of GnIH Gene and Its Correlation with Egg-laying Traits in Pigeons [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1412-1421.
[10]	ZHANG Fangwei, ZHANG Qi, ZHANG Yunpeng, LI Xin, ZHANG Jingbo, ZHANG Shumin, YU Yongsheng. Polymorphism of FOXA2 Gene Exon 3 and Its Association with Reproductive Traits in Songliao Black Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1444-1451.
[11]	TAN Yuancheng, LUO Hualun, ZHANG Yiyu, YANG Suan, WANG Yingtong, WANG Chunyuan. Polymorphism of MyoG Gene and Its Association with Body Size and Meat Traits in White Feather King Pigeons [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1461-1471.
[12]	HAN Dayong, ZHOU Chunbao, NI Ligang, CHEN Zhangyan, ZHAO Jinming. Genetic Diversity of mtDNA D-loop Sequence in Sujiang Pig [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1472-1479.
[13]	YANG Zhi, YU Jiaxin, YANG Linfu, WANG Kang, LI Guanzong, DUAN Gang, YANG Fumei, LIU Zhiqi, ZHOU Ke, HE Jun, DAI Feiyan. Study on the Therapeutic Effect of Modified Sini Decoction on Piglet Diarrhea [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1707-1717.
[14]	LI Dan, ZHANG Haisen, WANG Yiqun, GAO Dengke, ZHAO Hongcong, LI Chao, JIN Yaping, CHEN Huatao. Eukaryotic Expression Vector Construction,Bioinformatics and Tissue Expression Profiles Analysis of CRY1 Gene in Dairy Cows [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 870-881.
[15]	CHANG Yitong, ZHANG Wei, PENG Yinglin, CHEN Chen. Evolutionary Analysis,Target Gene Prediction and Tissue Expression Analysis of miR-192 [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 882-892.