关岭牛MyoD Ⅰ基因在不同组织和时期中的表达分析

doi:10.16431/j.cnki.1671-7236.2015.04.002

Abstract

Abstract: In order to study differential expression in different tissues and period of MyoD Ⅰ gene in Guanling cattle. This study detected the expression of MyoD Ⅰ in longest back muscle, leg muscles, heart, liver, fat and small intestine tissues of Guanling cattle by Real-time quantitative PCR. Meanwhile, analyzed the expressing rules of longest back muscle in foetal, 18 and 30 months. The results showed that the MyoD Ⅰ was expression at high level in longest back muscle and in muscle tissues after birth had high expression, with the extension of born showed an upward trend. It was inferred that the reason of the high expression might be that longest back muscle was muscle enrichment place and increase of muscle fullness.

Key words: Guanling cattle; MyoD Ⅰ gene; Real-time quantitative PCR; expression level

CLC Number:

Q786

HUAN Cong-cong, XU Hou-qiang, CHEN Wei, CHEN Xiang, ZHAO Jia-fu, ZHANG Wen, ZHOU Di, XIA Dan. Expression Analysis on Different Tissue and Period of MyoDⅠ Gene in Guanling Cattle[J]. , 2015, 42(4): 782-788.

References

[1] Voronova A,Coyne E,Al Madhoun A,et al.Hedgehog signaling regulates MyoD expression and activity [J].Biol Chem,2013,288(6):4389-4404.
[2] 杨明娟,陈宏.牛MRFs基因家族研究进展[J].中国农业科学,2010,36(6):51-55.
[3] Hall J K,Banks G B,Chamberlain J S,et al.Prevention of muscle aging by myofiber-associated satellite cell transplantation[J].Sci Transl Med,2010,2(57):57.
[4] Van der Meer S F,Jaspers R T,Jones D A,et al.Time-course of changes in the myonuclear domain during denervation in young-adult and old rat gastrocnemius muscle[J].Muscle Nerve,2011,43(2):212-222.
[5] Malatesta M,Meola G.Structral and function alterations of the cell nucleus in skeletal muscle wasing:The evidence in situ[J]. Eur J Histochem,2010,54(4):44.
[6] Zhang B T,Yeung S S,Liu Y,et al.The effects of low frequency electrical stimulation on statellite on statellite cell activity in rat skeletal muscle during hindlimb suspension[J].BMC Cell Biol,2010,18(11):87.
[7] LI H,Zhu C,Tao Z,et al.MyoD and Myf6 gene expression patterns in skeletal muscle during embryonic and posthatch development in the domestic duck (Anas platyrhynchos domestica) [J].Journal of Animal Breeding and Genetics,2014,131(3):194-201.
[8] Soumillion A,Erkens J H F.Genetic variation in the porcine myogenin gene locus [J]. Mamm Genome,1997,8:564-568.
[9] Szuhai K,de Jong D,Leung W Y,et al.Transactivating mutation of the MyoD1 gene is a frequent event in adult spindle cell rhabdomyosarcoma[J].The Journal of Pathology,2014,232(3):300-307.
[10] Kohsaka S,Shukla N,Ameur N,et al.Acetyltransferase association and histone acetylation at the MyoD1 locus during myogenic differentiation[J].The FASEB Journal,2014,28(1):946.
[11] Akizawa Y,Kanno H,Kawamichi Y,et al.Enhanced expression of myogenic differentiation factors and skeletal muscle proteins in human amnion-derived cells via the forced expression of MyoD1[J]. Brain and Development,2013,35(4):349-355.
[12] Blum R,Dynlacht B D.The role of MyoD1 and histone modifications in the activation of muscle enhancers[J].Epigenetics,2013,8(8):778-784.
[13] 黄萌,许尚忠,昝林森,等.牛MyoD基因遗传变异及其对胴体性状的影响[J].中国畜牧兽医,2007,34(9):40-43.
[14] Yao Z Z,Fong A P,Cao Y,et al. Comparison of endogenous and overexpressed MyoD shows enhanced binding of physiologically bound sites[J].Skeletal Muscle,2013,3(1):1-9.
[15] 王秋华,曹允考,李树峰.牛MyoG基因启动子的克隆及功能的初步分析[J].畜牧兽医学报,2012,43(1):37-43.
[16] 李飞,许厚强,陈伟,等.关岭牛MyoD基因启动子报告质粒的构建及活性验证[J].基因组学与应用生物学,2013,4:459-466.
[17] 杨丽华,白东义,高雅罕,等.蒙古马运动相关基因的表达量差异研究[J].畜牧兽医学报,2013,44(12):1900-1906.
[18] 李蓓,何小龙,芒来.蒙古马突触融合蛋白17(STX17)基因多态性及在不同毛色皮肤组织中的表达分析[J].畜牧兽医学报,2011,42(11):1632-1637.
[19] 曹婷,侯冠彧,施力光,等.五指山猪不同组织中Myf5与MyoD1基因的表达研究[J].家畜生态学报,2012,5:12-15.
[20] 陈禧,刘贺贺,李亮,等.MyoD基因家族在鸭小肠组织发育中的表达研究[J].中国家禽,2010,8:20-23.
[21] 田璐,许尚忠,岳文斌,等.MyoD基因对肉牛胴体性状影响的分析[J].遗传,2007,29(3):313-318.
[22] Alves H J,Alvares L E,Gabriel J E,et al.Influence of neural tube/notochord complex on MyoD expression and cellular proliferation in chicken embryos[J].Braz J Med Biol Res,2003,36(3):191-197.
[23] Onur K,Julio J M,Shoko Y,et al.Progenitors of skeletal muscle satellite cells express the muscle determination gene,MyoD[J].Dev Bio,2009,332(3):131-141.
[24] 潘同斌,钱福鸿,韩天翼,等.大负荷运动后不同时间大鼠骨骼肌成肌调节因子MyoD、myogenin表达的变化[J].中国引动医学杂志,2011,30(10):926-930.

Expression Analysis on Different Tissue and Period of MyoDⅠ Gene in Guanling Cattle

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LYU Xiaonan, XU Lipu, ZHANG Wen, WANG Na, CAO Huan, WANG Xiaoliang, WANG Shu, WANG Jingbo. Research on Histopathological Changes and Virus Distribution Situation of Mirror Carps Infected with Carp Edema Virus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1077-1084.
[2]	ZHANG Junfang, SUN Jianfu, SUN Bin, TANG Lin, WANG Ying, WANG Enze, CUI Yan, LI Qiang, YAN Changguo, LI Xiangzi. Cloning and Bioinformatics Analysis of PPARγ Gene of Yanbian Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2695-2704.
[3]	LIU Pengcheng, ZHANG Ji, QIU Ganyuan, GONG Yu, LI Xuesong, LI Wei, ZHANG Yiyu, LIU Ruoyu. Polymorphism of Bovine TBC1D Gene and Its Association with Growth Traits in Guanling Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2853-2863.
[4]	MIAO Manning, GUO Yiwen, HU Debao, ZENG Yuhan, LI Xin, ZHANG Linlin, DING Xiangbin, GUO Hong. Transcriptome Sequencing and Bioinformatics Analysis of MSTN Gene-edited Bovine Muscles [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2271-2281.
[5]	LI Heng, ZI Xiangdong. Cloning and Expression in Cumulus-oocyte Complexes of Yak TEDDM1 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1284-1293.
[6]	CHEN Lei, YUAN Feng, HE Sangang, MAYILA, LI Wenrong. Cloning,Sequencing and Quantitative Expression of FGF10 Gene in Sheep During Hair Follicle Development Period [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(2): 425-432.
[7]	DAI Zhenglie, ZHU Jingjing, CHEN Zhenyu, ZHOU Xiaolong, WANG Han, LI Xiangchen, ZHAO Ayong, YANG Songbai. Cloning, Bioinformatics Analysis and Expression of Porcine SENP1 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(12): 4382-4390.
[8]	JIANG Yixuan, WANG Yaling, XING Shanshan, SONG Guohua, XU Huifen, LI Ming. Cloning, Bioinformatics Analysis and Expression Profile Construction of MSTN Gene in New Zealand White Rabbits [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3501-3511.
[9]	LI Zhiqiang, TAO Chenyu, WEI Aolong, JIA Qing. Establish on Real-time Quantitative PCR Method for Purity Detection of Porcine Sex-controlled Sperm [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3682-3690.
[10]	WANG Zhengfan, ZHU Lisai, WANG Juan, LI Yiyun, XIANG Rui, YING Biyun, WANG Guiping, JIA Aiqing, BAI Aiquan. Establishment and Preliminary Application of Duplex Real-time Quantitative PCR for Detection of PDCoV and TGEV [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3855-3863.
[11]	LI Anqi, JIANG Lei, SU Xiaotong, ZAN Linsen, WANG Hongbao. Expression Characteristics Analysis of ACTC1 Gene in Qinchuan Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2359-2367.
[12]	LI Jinghui, XING Siyuan, WANG Xicai, LI Qinghe, ZHAO Guiping, ZHANG Yonghong, WEN Jie, LIU Ranran. Screening of Candidate Genes Related to Intramuscular Fat Deposition in Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1828-1836.
[13]	ZHANG Ji, YI Ming, CHENG Chaoyou, CHENG Junhua, FU Zhengxian, YANG Dewen, WU Xue, QIU Ganyuan, LIU Ruoyu. Study on Tissue Expression of SOCS7 and GFAP Genes in Weining Sheep [J]. , 2019, 46(7): 1899-1906.
[14]	RUAN Yiqi, LI Dapeng, RAN Xueqin, MAO Ning, YI Fanli, ZHANG Fuping, WANG Jiafu. The Methylation in the Exon 1 of RARRES1 Gene and its Association with Gene Differential Expression of Xiang Pig Ovary [J]. , 2018, 45(6): 1471-1478.
[15]	HUA Min, HE Xinwei, WAN Run, CHENG Zhentao, ZHOU Bijun, WEN Ming. Sequence Analysis of Cofilin2 Gene and Its Expression in Different Tissues of Sansui Ducks [J]. , 2018, 45(4): 858-865.