羊口疮病毒的分离与鉴定

doi:10.16431/j.cnki.1671-7236.2015.02.036

Abstract

Abstract: One strain of virus was isolated by passage culture in bovine testis cell,lamb testis cell,MDBK cell and BHK-21 cell of lip crust tissue collected from suspected Orf sheep in Chifeng,Inner Mongolia.The isolated virus was identified through negative staining by TEM observation and PCR detection method.One pair of specific primers was designed and synthesized referring to nucleotide sequence of Orf virus (ORFV) ORF059 (F1L) gene in GenBank.F1L gene of isolated virus was successfully cloned and sequenced,the homology was analyzed with many reference strains.The result showed that typical brick-shaped virion was found by TEM observation,and the isolated strains had high homologies with reference strains,the homologies were all more than 96%,illustrating that the isolated strains was ORFV,named as OV/nm-hd.

Key words: Orf virus; isolation and identification; F1L gene; sequence analysis

CLC Number:

S852.65

PANG Fang-yuan, GAO Ri-ming, LI Xu-dong, YAN Cong, LI Hao, WANG Yan-jie, ZHANG Qi-jin. Isolation and Identification of Orf Virus[J]. , 2015, 42(2): 467-471.

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Isolation and Identification of Orf Virus

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