Kiss-1基因在不同发育阶段五指山猪睾丸中的表达及定位研究

doi:10.16431/j.cnki.1671-7236.2015.02.021

Abstract

Abstract: The research aimed to study the expression of Kiss-1 in different developmental stages of Wuzhishan pig testis.The expression of Kiss-1 mRNA in 30,60,80 and 120 day-old Wuzhishan pig testis were studied using Real-time PCR.Cellular localization of Kiss-1 in testis was examined by immunohistochemistry.The results showed that the expression of Kiss-1 mRNA was increased with the age before puberty (P<0.05),and reached the highest level in 80-day-old testis (P<0.05),then decreased at 120 days of age (P<0.05).There was a positive expression of Kiss-1 in interstitial tissue,spermiogonium,primary spermatocyte,second spermatocyte,and round spermatid of adult Wuzhishan pig testis.However,the Kiss-1 immunopositive production was not found in sperm.The results confirmed that Kiss-1 gene played important role in spermatogenesis of Wuzhishan pig.

Key words: Kiss-1; Wuzhishan pig; testis; Real-time PCR; immunohistochemistry

CLC Number:

S828

XUN Wen-juan, CAO Ting, ZHOU Han-lin, HOU Guan-he, SHI Li-guang. Study on Expression and Localization of Kiss-1 Gene in Different Developmental Stages of Wuzhishan Pig Testis[J]. , 2015, 42(2): 370-374.

References

[1] Gottsch M L,Clifton D K,Steiner R A.Kisspeptin-GPR54 signaling in the neuroendocrine reproductive axis[J].Mol Cell Endocrinol,2006(254-255):91-96.
[2] Irwig M S,Fraley G S,Smith J T,et al.Kisspeptin activation of gonadotropin releasing hormone neurons and regulation of Kiss-1 mRNA in the male rat[J].Neuroendocrinology,2004,80(4):264-272.
[3] Dhillo W S,Chaudhri O B,Patterson M,et al.Kisspeptin-54 stimulates the hypothalamic-pituitary gonadal axis in human males[J].J Clin Endocrinol Metab,2005,90(12):6609-6615.
[4] 冯涛,储明星,张英杰.Kiss-1/GPR54基因及其在生殖中的作用[J].遗传,2008,30(4):419-425.
[5] 孙兆贵,顾正,王健.Kiss-1/GPR54系统的生殖内分泌功能研究进展[J].生殖与避孕,2009,29(6):374-384.
[6] 王雪松.Kiss-1/GPR54系统在雄性大鼠生殖轴系功能研究[D].南京:南京医科大学,2007.
[7] 刘红羽,王军,吕文发.Kisspeptin在山羊生殖器官的分布定位[A].中国畜牧兽医学会动物繁殖学分会第十六届学术研讨会论文集[C].2012.
[8] 邵宝平,王继卿,罗玉柱,等.Kiss-1基因在黄牛精子发生中的表达[J].中国兽医科学,2011,41(12):1282-1287.
[9] Gottsch M L,Cunningham M J,Smith J T,et al.A role for kisspeptins in the regulation of gonadotropin secretion in the mouse[J].Endocrinology,2004,145(9):4073-4077.
[10] Smith J T,Clay C M,Caraty A,et al.Kiss-1 messenger ribonucleic acid expression in the hypothalamus of the ewe is regulated by sex steroids and season[J].Endocrinology,2007,148(3):1150-1157.
[11] Tena-Sempere M.Kisspeptin signaling in the brain:Recent developments and future challenges[J].Mol Cell Endocrinol,2010,314(2):164-169.
[12] Yasuko T,Satoshi K,Yoshihiro T,et al,Expression of Kiss-1,a metastasis suppressor gene,in trophoblast giant cells of the rat placenta[J].Biochim Biophys Acta,2004,1678(2-3):102-110.
[13] Navarro V M,Fernandez-Femandez R,Castellano J M,et al.Advanced vaginal opening and precocious activation of the reproductive axis by Kiss-1 peptide,the endogenous ligand of GPR54[J].J Physiol,2004,561(2):379-386.
[14] Navarro V M,Castellano J M,Fernndez-Fernndez R,et al.Developmental and hormonally regulated messenger ribonucleic acid expression of Kiss-1 and its putative receptor,GPR54,in rat hypothalamus and potent luteinizing hormone-releasing activity of Kiss-1 peptide[J].Endocrinology,2004,145(10):4565-4574.
[15] 李万宏,王春强,周虚.抑制素对动物生殖系统的影响及其在动物生产中的应用[J].中国畜牧兽医,2011,38(6):204-207.
[16] 成令忠,钟翠平,蔡文琴.现代组织学[M].上海:上海科学技术文献出版社,2003.

Study on Expression and Localization of Kiss-1 Gene in Different Developmental Stages of Wuzhishan Pig Testis

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	DU Zhaohui, GE Zihao, YOU Junyi, LIU Xiaowei, LIANG Guodong, SUN Shiduo, HU Jianhong, LI Xiao. Effects of Clostridium butyricum and Sodium Butyricum on Reproductive System Injury Induced by High Fat Diet in Male Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1603-1616.
[2]	WANG Guang, ZOU Jiahao, ZHANG Yongtao, LI Dexian, LI Xueqing, YU Mengqi, CHEN Lu, YUAN Yuxin, LI Guang. Difference Analysis of Metabolites in Testis of Guanzhong Dairy Goats at Different Developmental Stages [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3453-3464.
[3]	ZHAO Fengmiao, GUO Ting, ZHOU Yaping, ZHAO Hongmei, WANG Yuchen, TIAN Guangyuan, SUN Yajie, BIAN Yuchen, YU Jialiang, HAO Yongqing. Pathogenicity Analysis of Bovine Viral Diarrhea Virus and Immune Effect Evaluation of E2 Protein in New Zealand White Rabbits [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3549-3558.
[4]	TIE Yanan, YANG Xixi, WANG Zhiliang, WANG Xinrong. Morphological and Histological Characteristics of Testis and Epididymis of Hyla Rabbits at Different Ages Before Sexual Maturity [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2645-2653.
[5]	HU Wen, HUO Konglin, SONG Xingxing, ZHANG Xin, ZHANG Duoni, LUO Rongrong, XU Wenhao, LI Xun. Effects of Gonadotropin Inhibitory Hormone on Gonadal Reproductive Function and Glucose Metabolism in SD Rats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2239-2249.
[6]	MA Baoyi, SHENG Chenyan, LIU Hongying, MA Qingxia, WANG Tingting, LI Jianming, SHI Kun, DU Rui, LENG Xue. Establishment of a Duplex TaqMan Real-time PCR Method for Detection of Bovine Viral Diarrhea Virus Types 1 and 2 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2326-2335.
[7]	LYU Xiaonan, XU Lipu, ZHANG Wen, WANG Na, CAO Huan, WANG Xiaoliang, WANG Shu, WANG Jingbo. Research on Histopathological Changes and Virus Distribution Situation of Mirror Carps Infected with Carp Edema Virus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1077-1084.
[8]	LIU Zaixia, DUAN Shi, SUN Yanyong, LYU Qi, FU Shaoyin, HE Xiaolong, ZHANG Wenguang, LIU Yongbin. Study on Differential Gene and Protein Interaction Network of Testis in Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 1-11.
[9]	GUO Yan, DING Wei, TAO Jinzhong, CHEN Shaoyu, YUAN Ligang, LI Yingkang. Comparison of Skin Histochemical Characteristics in Tan Sheep at Different Ages [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2762-2770.
[10]	LI Jianmei, JI Zhengjian, LIU Mei, SHEN Xinyue, CHENG Xu, YU Yan, DAI Yabin. Expression Changes of Immune-related Genes in Tibetan Chickens and Recessive White Chickens Following Eimeria tenella Infection [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1440-1448.
[11]	LIU Rui, LI Jiangling, CHEN Xiaohui, ZHAO Sujun, WANG Qiushi, HE Zhiping, WANG Yuping. Identification of circRNA Related to Male Reproduction in Pig Testis [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3660-3669.
[12]	REN Jianming, WEI Yanming, YUAN Ligang, WANG Qianmei, LI Chengye. Comparison of the Distribution of PGP9.5 and NPY in Cryptorchidism and Testicular Tumors in Dogs [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(9): 2741-2750.
[13]	CHEN Yun, WANG Kai, ZHOU Lele, HUANG Jianhao, YE Chao, WEI Hengxi, LI Li, ZHANG Shouquan. Expression of OPN in Germ Cells of Landrace Boar and Its Correlation with Reproductive Performance [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2536-2543.
[14]	MU Songyin, XIONG Xianrong, MA Hongcheng, HAI Zhuo, LI Jian. Cloning of L-PGDS Gene and Its Expression Pattern in Different Tissues and Testis at Different Development Stages in Cattle-yak [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(12): 3985-3992.
[15]	JIANG Hui, LIU Pan, LI Yuanyuan, LIU Kuiguo, HUANG Xixia, LIU Jiangwei, XU Lei, DU Jianwen, SANG Zhagen, YANG Xuemei, ZHAO Fanfan, WANG Dan. Expression and Bioinformatics Analysis of Mastitis Resistance Related Genes in Xinjiang Brown Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(1): 90-97.