急性死亡猪中猪丹毒杆菌的分离鉴定

doi:10.11843/j.issn.1671-7236.2015.03.038

Abstract

Abstract: Two strains of swine Erysipellthrix rhusiopathiae,which were characterized by cultural character,shape observation,biochemical test,polymerase chain reaction detection,animal pathogenicity experiment,antibiotic sensitivity test and MIC determination,were isolated from infected pig farms in Jiangxi province.The animal pathogenicity experiment demonstrated that 1×10⁸ CFU could be lethal to mice.The antibiotic sensitivity test result showed that the two strains were highly sensitive to β-lactam antibiotics,but resistant to most of the other antibiotics.The MIC test displayed that the disinfectants used in this farm were not effective anymore.This result was important for clinic to prevent and treat swine erysipelas.

Key words: swine Erysipellthrix rhusiopathiae; isolation and identification; antibiotic sensitivity test

CLC Number:

S852.61

ZENG Wen-bin, LIU Yue-xin, WANG Ping, XING Wen-ding, ZUO Ming-kai, TAO Zheng, BAI Shuai-zhou. Isolation and Identification of Swine Erysipellthrix rhusiopathiae in Acute Cases[J]. , 2015, 42(3): 752-756.

References

[1] 郭良兴,陈克研,赵魁,等.猪丹毒杆菌的分离鉴定及耐药性试验[J].中国畜牧兽医,2011,38(4):199-202.

[2] 宣长和.猪病学[M].第2版.北京:中国农业科学技术出版社,2003.

[3] Veraldi S,Girgenti V,Dassoni F,et al.Erysipeloid: A review[J].Clin Exp Dermatol,2009,34(8):859-862.

[4] Wang Q,Chang B J,Riley T V.Erysipelothrix rhusiopathiae[J].Vet Microbiol,2010,140(3-4):405-417.

[5] 毛江昌,李军星,袁秀芳,等.规模猪场猪丹毒的诊治[J].浙江农业科学,2012,9:1315-1318.

[6] 车勇良,陈如敬,王隆柏,等.猪丹毒杆菌的分离鉴定及其SpaA基因的遗传变异分析[J].中国兽医学报,2011,11:1591-1593.

[7] 东秀珠,蔡妙英.常见细菌系统鉴定手册[M].第3版.北京:科学出版社,2001.

[8] 谭侃侃.丹毒丝菌的分离鉴定及其SpaA-N蛋白和lipo蛋白的免疫原性分析[D].长沙:湖南农业大学,2011.

[9] Clinical and Laboratory Standards Institute/NCCLS.Performance standards for antimicrobial susceptibility testing,fifteenth informational supplement.CLSI/NCCLS document M100-S15[S].Wayne,Pennsylvania,Clinical and Laboratory Standards Institute,2005.

[10] 冷闯,邓舜洲,张文波,等.一例母猪蓝耳病继发感染猪丹毒的诊疗报告[J].养猪,2013,2:128.

[11] 胡云霏,钱幸,王淑琴,等.猪血清中抗猪丹毒丝菌特异性IgG的纯化[J].湖南农业大学学报(自然科学版),2013,2:183-187.

[12] 罗银珠,刘树中,杜宗亮,等.猪丹毒的紧急处理及防控措施[J].中国猪业,2012,1:39-41.

[13] 宋培武,朱巧玲.警惕当前猪丹毒在规模猪场的流行与危害[J].养猪,2013,5:117-118.

[14] 邱美珍,杜丽飞,杨爱梅,等.猪丹毒杆菌临床分离鉴定与药敏试验[J].养猪,2011,6:111-112.

[15] 梁跃,徐引弟,杨志昆,等.关节炎患猪中猪丹毒丝菌的分离与鉴定[J].河南农业科学,2012,11:136-138.

[16] 杨佳冰,吴亚文,王晓亮,等.一例病死猪猪丹毒杆菌分离鉴定及药敏试验[J].黑龙江畜牧兽医,2014,2:73-74.

Isolation and Identification of Swine Erysipellthrix rhusiopathiae in Acute Cases

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LU Yukui, LIU Jiaqi, ZHONG Jiacheng, CHEN Jidang, ZHU Wanjun, ZHANG Yishan, ZHANG Jipei. Isolation,Identification and Pathogenicity Analysis of Avibacterium paragallinarum from Geese [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2562-2572.
[2]	LI Zhijie, ZHAO Ying, MA Xin, WANG Meng, YU Sijiu, WANG Libin, PAN Yangyang. Research Progress on Isolation Methods and Main Reproductive Functions of Exosomes from Animal Follicular Fluid [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1480-1488.
[3]	TIAN Changqing, ZHANG Kunzhong, QI Yumei, SHI Wenjing, DONG Zhijie, ZHANG Haohao, HE Zengwen, ZHI Ji, ZHAO Xuehui, CHONG Qian, XUE Huiwen, GOU Huitian. Isolation,Identification and Biological Characteristics Analysis of a Strain of Listeria monocytogenes from Environment [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1543-1555.
[4]	CAO Yingying, LIANG Liang, ZENG Yirong, ZHONG Qinghua, YUAN Xiaofang, TANG Haibo, LENG Jing. Isolation,Identification and Biological Characteristics of Salmonella from Tupaia belangeri chinensis [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1718-1728.
[5]	WANG Bingyi, MA Hongcai, ZOU Minghao, FAN Shijie, YUAN Zhenjie, Labaciren, DONG Hailong, ZENG Jiangyong. Isolation,Identification and Drug Resistance Analysis of Streptococcus from Yak [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 745-753.
[6]	ZHONG Huachen, WANG Lifang, GUO Chenyang, LIU Jialin, SONG Jie. Isolation,Identification and Drug Resistance Analysis of Pathogenic Bacteria from Milk Samples and Environment of Mastitis in Inner Mongolia [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 817-826.
[7]	SUN Yu, ZHANG Hao, LI Changyang, TAO Yanzi, WANG Qiuju. Isolation,Identification and Biological Characteristics of Antioxidant Bacillus from Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 359-367.
[8]	WEI Jin, WANG Jia, ZHAO Haoran, LIU Bo, LIU Yuanjie, YAO Wensheng, LIU Yan, MA Xin. Isolation, Identification and Antimicrobial Susceptibility of Mycoplasma gallisepticum from Different Areas [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 368-376.
[9]	YANG Fumei, YANG Linfu, ZENG Cheng, YANG Zhi, WANG Kang, DUAN Gang, DAI Feiyan. Isolation,Identification and Biological Characterization Analysis of a Strain of Carnobacterium maltaromaticum from Barking Deer [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 398-407.
[10]	ZHANG Wei, WU Hua, YIN Yajie, LI Songli, HOU Shaohua. Isolation and Identification of Novel Duck Reovirus and Its σC Gene Sequence Analysis [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3520-3529.
[11]	XI Jing, YI Jihai, WANG Yueli, XU Zhenyu, LI Peidong, ZHANG Jiangwei, CHEN Chuangfu. Isolation, Identification and Genotype Analysis of Group A Bovine Rotavirus G10 Strain XJ-2022 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3530-3538.
[12]	LIU Xiaolan, LIU Changjin, YU Wenyang, LI Xiaoxiang, BIAN Yanchao, HUANG Xiaohua, LUO Feng, DENG Shunzhou. Isolation and Identification of Porcine Rotavirus Jiangxi Strain AY01 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3151-3162.
[13]	XU Minsheng, KE Haiyi, SHI Keda, YANG Dongxia, ZHAI Shaolun, ZANG Yingan, LI Chunling. Isolation and Identification of Actinobacillus pleuropneumoniae from Guangdong Province and Detection and Analysis of Drug Resistance Phenotype and Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3212-3225.
[14]	YANG Zemin, LI Shuang, JIN Zhengyu, LIAO Yixiao, YANG Ying, WEN Ming. Isolation,Identification and Biological Characteristics of a Strain of Lactobacillus plantarum from Carp [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2805-2811.
[15]	YIN Yajie, LIANG Ruiying, CUI Huan, MENG Lijia, NI Weiling, QIAO Sina, GUO Kangkang, ZHANG Bo, LI Songli, HOU Shaohua, DONG Shishan. Isolation,Identification of Duck Tembusu Virus,and Genetic and Evolutionary Analysis of Its Envelope Protein [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1497-1506.