›› 2015, Vol. 42 ›› Issue (3): 569-574.doi: 10.11843/j.issn.1671-7236.2015.03.010

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Application of Hydroformylated Guinea Pig Red Blood Cells in Hemagglutination Test for Human Influenza Virus

WAN Yong-hu1, ZHANG De-zhu1, ZHENG Qin-ni1, ZHUANG Li1, FU Lin1, REN Li-juan1, YANG Tao-mei2, TANG Guang-peng1, WANG Zhao-xiao1   

  1. 1. Guizhou Center for Disease Control and Prevention, Guiyang 550004, China;
    2. College of Medicine, Shandong University, Jinan 250012, China
  • Received:2014-09-25 Online:2015-03-20 Published:2015-03-30

Abstract: Hemagglutination test was a conventional detection method in epidemic surveillance of influenza.In order to overcome the defects of fresh red blood cells for troublesome and time-consuming preparation,easy hemolysis,short time conservation as well as chicken red blood cells could not agglutinate influenza virus "O" phase strains,our research optimized the method and the condition of preparation of hydorfomylated guinea pig red blood cells through HA test for different types of influenza virus and the fresh guinea pig red blood cells were taken as control.The results showed that guinea pig red blood cells hydorfomylated by glutaraldehyde were anhemolytic,and it had a good stability and a long time conservation.Compared with fresh guinea pig red blood cells,the effect of hemagglutination activity was almost identical.

Key words: influenza virus; hydroformylated red blood cells; hemagglutination test

CLC Number: