视黄酸诱导基因-Ⅰ在家禽天然免疫应答中作用的研究进展

doi:10.16431/j.cnki.1671-7236.2018.12.021

摘要/Abstract

摘要：

家禽的天然免疫应答在抵抗病毒感染的过程中起着关键性作用，视黄酸诱导基因-Ⅰ（retinoic acid inducible gene-Ⅰ，RIG-Ⅰ）作为细胞质内一类识别病毒双链RNA的模式识别受体，与天然免疫应答密切相关。它可通过RNA配体结合病原相关分子模式监测细胞质中的病毒RNA，此过程激活了RIG-Ⅰ及下游线粒体抗病毒信号蛋白（MAVS），最终导致干扰素调节因子（IRF3/7）和核因子κB （NF-κB）活化，诱导产生Ⅰ型干扰素等免疫细胞因子，进而使细胞做出相应的抗病毒天然免疫反应。但由于鸡体内缺乏RIG-Ⅰ基因，目前大多将鸭源或鹅源RIG-Ⅰ基因转染鸡成纤维母细胞（DF-1）研究RIG-Ⅰ基因在鸡感染禽类病毒时是否具有免疫功能。文章介绍了RIG-Ⅰ在家禽体内的表达及其介导的抗病毒天然免疫信号通路，并简述了RIG-Ⅰ在家禽体内抗病毒作用的研究概况，为抑制家禽病毒的感染和免疫系统研究，以及研制新型抗病毒疫苗或免疫佐剂等提供参考。

关键词: 视黄酸诱导基因-Ⅰ(RIG-Ⅰ); 家禽; 天然免疫应答; 抗病毒

Abstract:

The innate immune response of poultry plays a key role in protecting the host from viral infections.Retinoic acid inducible gene-Ⅰ (RIG-Ⅰ) is used as a class of cytoplasmic internal recognition receptor of viral double-stranded RNA which is closely related to the natural immune response.It can monitor viral RNA in the cytoplasm by RNA ligand binding to pathogen-associated molecular patterns,and that process can activate RIG-Ⅰ and downstream mitochondrial antiviral signaling protein (MAVS),eventually leading to the activation of interferon regulatory factor (IRF3/7) and NF-κB,induce the production of type Ⅰ interferon and other immune cytokines,and then the cells make a corresponding anti-viral natural immune response.However,due to the lack of RIG-Ⅰ gene in chickens,the current researches are most to transfer RIG-Ⅰ gene from duck or geese source to chicken fibroblasts (DF-1) to study whether RIG-Ⅰ gene has immune function in chicken infected avian viruses.This article describes the expression of RIG-Ⅰ in poultry and its mediated antiviral innate immune signaling pathway,outlines the research status of antiviral effects of RIG-Ⅰ in poultry to provide references in the study of infection and immune system of poultry and the development of new anti-virus vaccines and immune adjuvants.

Key words: RIG-Ⅰ; poultry; natural immune response; antiviral

中图分类号:

S852.4

李雪峰, 任梦婷, 许丽文, 王芳芳. 视黄酸诱导基因-Ⅰ在家禽天然免疫应答中作用的研究进展[J]. 《中国畜牧兽医》, 2018, 45(12): 3479-3485.

LI Xuefeng, REN Mengting, XU Liwen, WANG Fangfang. Research Progress on Role of Retinoic Acid Inducible Gene-Ⅰ in Innate Immune Response of Poultry[J]. , 2018, 45(12): 3479-3485.

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