直接法与替代法测定肉羊氨化秸秆代谢能值的比较研究

doi:10.16431/j.cnki.1671-7236.2018.06.009

摘要/Abstract

摘要：

试验旨在对直接法与替代法测定肉羊氨化秸秆代谢能值进行比较。将4月龄、体重25 kg左右，体况良好的杜泊公羊20只随机分为5组，每组4个重复，每个重复1只羊。直接法组（全氨化秸秆组）饲喂全氨化玉米秸秆，替代法组饲喂用不同比例氨化秸秆替代玉米和豆粕的基础日粮，即：饲喂基础日粮（精粗比1：1），饲喂氨化秸秆替代比例为20%、40%和60%的试验日粮。采用体内法进行动物消化代谢和呼吸测热试验，试验期60 d，含预饲期15 d，正试期45 d，分为3期，每期15 d。结果显示：替代法各组日粮的干物质（DM）、粗蛋白质（CP）、有机物（OM）表观消化率均随着替代比例的增加而显著降低（P<0.05），但均显著高于直接法组（P<0.05）。替代法各组的消化能（DE）与代谢能（ME）均显著高于直接法组（P<0.05）。通过比较替代法组内的消化能与代谢能发现，基础日粮组 > 20%替代组 > 40%替代组 > 60%替代组。而在对原料氨化秸秆的营养物质消化率测定及能值评定中，直接法全氨化秸秆组的DM、OM表观消化率及DE、ME均显著高于60%替代组（P<0.05），但与其他替代组无显著差异（P>0.05），且均接近于20%替代组。综上所述，采用直接法和替代法对氨化玉米秸秆进行能值评定时，替代比例不同会对试验结果造成显著差异。而通过替代法测定原料氨化秸秆的能量代谢时发现，氨化玉米秸秆适宜替代比为20%。

关键词: 氨化秸秆; 直接法; 替代法; 肉羊; 能量代谢

Abstract:

The purpose of the experiment was to compare the energy metabolism of ammoniated maize stalk in sheep by different methods (the direct method and the substitute method).20 4-month-old healthy Dorper rams about 25 kg were chosen and divided into 5 groups with 4 replicates per group and 1 ram per replicate.The rams in direct method group (full ammoniated maize straw group) fed only ammoniated maize stalk, and the substitute groups fed with different ratios of ammoniated maize stalk substitutes in basal diet (0, 20%, 40% and 60%).The in vivo method was used to determine animal digestion metabolism and respiratory calorie.The test period was 60 d including the pre-feeding period of 15 d,the official trial period of 45 d with three phases and 15 d per phase.The results showed that in the substitute method groups,the apparent digestibility of DM,CP and OM of the diets significantly decreased with the increase of the substitution ratio (P<0.05),but all of them were significantly higher than that of the direct method group (P<0.05).The values of DE and ME in the substitute method groups were also significantly higher than those in the direct method group (P<0.05).By comparing the DE and ME among the substitute method groups,the DE and ME was decrease in turn with increase of the substitution ratio.However,in the determination of the digestibility and energy assessment of the ammoniated maize stalk as the raw material,the apparent digestibility of DM and OM,DE and ME in the direct method group were significantly higher than those in 60% replacement group (P<0.05), but there was no significant difference with other substitute groups (P>0.05).In conclusion,when the direct method and the substitute method were used to evaluate the assessment of energy of ammoniated maize stalks, the difference of substitution ratio could make a significant difference to the results.When the energy metabolism of ammoniated maize stalk was determined by the substitute method, the optimum ammoniated maize stalk replacement ratio was 20%.

Key words: ammoniated maize straw; direct method; substitution method; mutton sheep; energy metabolism

中图分类号:

S816.5

姜雨轩, 张美美, 孙悠然, 谢小来, 于满满. 直接法与替代法测定肉羊氨化秸秆代谢能值的比较研究[J]. 《中国畜牧兽医》, 2018, 45(6): 1493-1501.

JIANG Yuxuan, ZHANG Meimei, SUN Youran, XIE Xiaolai, YU Manman. Comparison of Energy Metabolism of Ammoniated Maize Stalk in Mutton Sheep Determined by Direct and Substitution Methods[J]. , 2018, 45(6): 1493-1501.

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