橄榄苦苷对小鼠脑缺血再灌注损伤的保护作用

doi:10.16431/j.cnki.1671-7236.2016.09.025

摘要/Abstract

摘要：

本研究为探讨橄榄苦苷联合依达拉奉对小鼠脑缺血再灌注损伤的影响及其保护作用机制，将50只健康小鼠分成假手术组、模型组、橄榄苦苷组、依达拉奉组、橄榄苦苷＋依达拉奉组。利用双侧颈总动脉结扎方法制备慢性脑缺血再灌注小鼠模型，造模后药物处理21 d，用放射免疫法检测脑组织肿瘤坏死因子α（TNF-α）、白细胞介素1（IL-1β）、白细胞介素10（IL-10）含量，用比色法检测脑组织ATP酶、髓过氧化物酶（MPO）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活性及丙二醛（MDA）含量，用免疫组织化学法及Western blotting法检测大脑皮层脑源性神经营养因子（BDNF）的表达。结果显示，与假手术组相比，模型组脑组织TNF-α、IL-1β、MDA的含量及MPO活性极显著升高（P<0.01），IL-10、ATP酶、SOD、CAT水平均极显著降低（P<0.01），大脑皮层BDNF表达水平极显著降低（P<0.01）。与模型组比较，橄榄苦苷或依达拉奉治疗后，脑组织TNF-α、IL-1β、MDA的含量及MPO活性极显著降低（P<0.01），IL-10、ATP酶、SOD、CAT水平均极显著升高（P<0.01），大脑皮层BDNF表达水平极显著升高（P<0.01）。橄榄苦苷和依达拉奉联合治疗后脑缺血再灌注损伤的恢复更加显著。综上所述，橄榄苦苷和依达拉奉对小鼠脑缺血再灌注损伤具有明显的保护作用，其机制可能与改善神经功能、减少自由基损伤和抑制炎症因子水平有关，橄榄苦苷和依达拉奉联合治疗可以发挥更好的作用。

关键词: 橄榄苦苷; 脑缺血再灌注损伤; 依达拉奉; 炎症因子; 能量代谢障碍; 抗氧化能力; 脑源性神经营养因子

Abstract:

To explore the protection role and mechanism of oleuropein and edaravone on cerebral ischemia-reperfusion injury in mice.Fifty mice were divided into five groups:Sham operation group,model group,oleuropein group,edaravone group,oleuropein+edaravone group.The model was established by ligating common carotid artery.After modeling,the mice were administrated with oleuropein,edaravone and oleuropein+edaravone for 21 d,respectively.The contents of TNF-α,IL-1β and IL-10 were detected by radioimmunoassay.The activities of ATPase,MPO,SOD and CAT and MDA content were measured by spectrophotometry.The changes of BDNF expression in cerebral cortex were analyzed by immunohistochemistry and Western blotting.The results showed that compared to sham operation group,the contents of TNF-α,IL-1β,MDA and MPO activity in model group were extremely significantly increased (P<0.01),and the levels of IL-10,ATPase,SOD and CAT in model group were extremely significantly reduced (P<0.01),the BDNF expression in model group was extremely significantly decreased (P<0.01).Compared to model group,the contents of TNF-α,IL-1β,MDA and MPO activity in oleuropein,edaravone,and oleuropein+edaravone groups were extremely significantly decreased (P<0.01),the levels of IL-10,ATPase,SOD and CAT were extremely significantly increased (P<0.01),the BDNF expression was extremely significantly up-regulated in cerebral cortex of treatment group (P<0.01).Furthermore,the oleuropein+edaravone combined administration showed a better effect.The treatments of oleuropein and edaravone had a protective effect on cerebral ischemia reperfusion injury,the mechanisms of which might depend on improving neurological function,reducing free radical lesion and inhibiting inflammatory response.Moreover,the oleuropein+edaravone combination therapy might have an additive effect.

Key words: oleuropein; cerebral ischemia-reperfusion injury; edaravone; inflammatory factors; energy metabolism dysfunction; antioxidant capacity; BDNF

中图分类号:

R285

王昱, 秦序. 橄榄苦苷对小鼠脑缺血再灌注损伤的保护作用[J]. 《中国畜牧兽医》, 2016, 43(9): 2388-2394.

WANG Yu, QIN Xu. Protective Effects of Oleuropein on Cerebral Ischemia-reperfusion Injury in Mice[J]. , 2016, 43(9): 2388-2394.

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