纳米铜、灵芝孢子粉与大豆异黄酮对1~30日龄清远麻鸡生长、免疫及抗氧化性能的影响

doi:10.16431/j.cnki.1671-7236.2021.07.016

中国畜牧兽医 ›› 2021, Vol. 48 ›› Issue (7): 2415-2423.doi: 10.16431/j.cnki.1671-7236.2021.07.016

纳米铜、灵芝孢子粉与大豆异黄酮对1~30日龄清远麻鸡生长、免疫及抗氧化性能的影响

王一冰¹, 邝智祥², 叶金玲¹, 范秋丽¹, 张盛¹, 蒋守群¹

1. 广东省农业科学院动物科学研究所, 畜禽育种国家重点实验室, 农业农村部华南动物营养与饲料重点实验室, 广东省畜禽育种与营养研究重点实验室, 广州 510640;
2. 广东爱健康生物科技有限公司, 清远 511500

收稿日期:2020-12-08 出版日期:2021-07-20 发布日期:2021-07-15
通讯作者: 蒋守群 E-mail:jsqun3100@sohu.com
作者简介:王一冰(1990-),女,山东青岛人,副研究员,研究方向:黄羽肉鸡营养与免疫,E-mail:wangyibing77@163.com
基金资助:
国家重点研发计划（2018YFD0500600）；国家肉鸡产业技术体系项目（CARS-41-G10）；国家自然科学基金青年基金（31802104）；广东省科技计划项目（2017B020202003，2019A050505007）；广州市科技计划重点项目（201804020091）；广东省农业科学院院长基金项目（201908）；科技创新战略专项资金"高水平农科院建设"（R2019PY-QF008、R2018QD-076）

Effects of Nano Copper,Spores of Ganoderma lucidum and Soybean Isoflavone on Performance of Growth,Immune and Antioxidation of Qingyuan Partridge Chickens from 1 to 30 Days

WANG Yibing¹, KUANG Zhixiang², YE Jinling¹, FAN Qiuli¹, ZHANG Sheng¹, JIANG Shouqun¹

1. State Key Laboratory of Livestock and Poultry Breeding, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
2. Guangdong Aijiankang Biotechnology Co., Ltd., Qingyuan 511500, China

Received:2020-12-08 Online:2021-07-20 Published:2021-07-15

摘要/Abstract

摘要： 本研究对比分析了饲粮中添加纳米铜、灵芝孢子粉与大豆异黄酮对育雏阶段清远麻鸡生长性能、免疫功能及抗氧化性能的影响，旨在为肉鸡生产中添加剂的开发及应用提供参考。采用单因素随机分组设计，选用1 200只1日龄清远麻鸡母鸡分成4组，对照组（CON）饲喂基础饲粮，3个处理组（NC、GLS及SI）分别在基础饲粮中添加2 500 mg/kg纳米铜、600 mg/kg灵芝孢子粉和300 mg/kg大豆异黄酮预混剂。每个处理10个重复，每个重复30只鸡，试验期30天。试验结束后，采集试验鸡血浆、空肠黏膜样品，测定血液抗氧化相关生化指标及肠道免疫因子。结果表明：与对照组相比，①饲粮添加纳米铜、灵芝孢子粉与大豆异黄酮对清远麻鸡肉仔鸡的生长性能均无显著影响（P>0.05）。②灵芝孢子粉显著提高了30日龄清远麻鸡的胸腺比例（P<0.05）。③纳米铜显著提高了清远麻鸡的空肠绒毛隐窝比（P<0.05）。④纳米铜、灵芝孢子粉与大豆异黄酮均显著增加了清远麻鸡血浆和空肠中总超氧化物歧化酶（T-SOD）活性（P<0.05）；纳米铜显著增加了血浆谷胱甘肽过氧化氢酶（GSH-Px）活性（P<0.05）、降低了空肠黏膜丙二醛（MDA）含量（P<0.05）；大豆异黄酮显著降低血浆与空肠MDA含量（P<0.05）。⑤纳米铜组清远麻鸡空肠中免疫球蛋白A（IgA）含量显著增加（P<0.05），灵芝孢子粉组清远麻鸡血浆中肿瘤坏死因子-α（TNF-α）、空肠中干扰素-γ（IFN-γ）含量显著降低（P<0.05）。在本试验条件下，纳米铜、灵芝孢子粉与大豆异黄酮对清远麻鸡肉仔鸡生长性能没有显著影响，但均可改善抗氧化性能指标；纳米铜可提高空肠绒毛隐窝比，纳米铜与灵芝孢子粉可调节免疫因子分泌。其中纳米铜对抗氧化性能的调节作用最优，灵芝孢子粉对免疫功能调节作用最优。

关键词: 清远麻鸡; 纳米铜; 灵芝孢子粉; 大豆异黄酮; 抗氧化能力; 免疫功能

Abstract: This experiment was conducted to investigate the effects of nano copper,spores of Ganoderma lucidum and soybean isoflavone on growth performance,immune function and antioxidant capacity of Qingyuan Partridge chickens,as to provide reference for the development and application of additives in broiler production.A total of 1 200 1 d chicks were randomly assigned to 4 groups with 10 replicates of 30 birds per replicate.The control group was fed with basal diet (CON),and the birds in treatment groups were supplemented with 2 500 mg/kg nano copper (NC),600 mg/kg spores of Ganoderma lucidum (GLS) and 300 mg/kg soybean isoflavone premix (SI) for 30 d,respectively.At the end of the experiment,plasma and jejunal mucosa were collected to determine the biochemical variables related to antioxidation in plasma and intestinal immune factors.The results showed that,compared with the control group,①the growth performance of chickens was not affected by the three bioactive additives (P>0.05).②GLS increased the thymus ratio (P<0.05).③NC significantly increased the villus to crypt ratio in jejunum.④NC increased activity of glutathione catalase (GSH-Px) in plasma (P<0.05),and decreased jejunal MDA content (P<0.05) significantly.SI significantly reduced both plasmatic and jejunal malondialdehyde (MDA) content (P<0.05),and all three additives increased activity of total superoxide dismutase (T-SOD) in plasma and jejunum (P<0.05) significantly.⑤NC increased immunoglobulin (IgA) content in jejunum (P<0.05),and GLS decreased tumor necrosis factor-α (TNF-α) in plasma and interferon (IFN-γ) in jejunum (P<0.05)significantly.Based on this experiment,NC,GLS and SI did not affect the growth performance of Qingyuan Partridge chickens,while improved the antioxidant capacity.NC increased the villi/crypt ratio,and NC and GLS regulated the secretion of immune factors.Among them,NC had the best effect on antioxidant capacity and GLS had the best effect on immune function.

Key words: Qingyuan Partridge chicken; nano copper; spores of Ganoderma lucidum; soybean isoflavone; antioxidant capacity; immune function

中图分类号:

S816.7

王一冰, 邝智祥, 叶金玲, 范秋丽, 张盛, 蒋守群. 纳米铜、灵芝孢子粉与大豆异黄酮对1~30日龄清远麻鸡生长、免疫及抗氧化性能的影响[J]. 中国畜牧兽医, 2021, 48(7): 2415-2423.

WANG Yibing, KUANG Zhixiang, YE Jinling, FAN Qiuli, ZHANG Sheng, JIANG Shouqun. Effects of Nano Copper,Spores of Ganoderma lucidum and Soybean Isoflavone on Performance of Growth,Immune and Antioxidation of Qingyuan Partridge Chickens from 1 to 30 Days[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2415-2423.

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纳米铜、灵芝孢子粉与大豆异黄酮对1~30日龄清远麻鸡生长、免疫及抗氧化性能的影响

Effects of Nano Copper,Spores of Ganoderma lucidum and Soybean Isoflavone on Performance of Growth,Immune and Antioxidation of Qingyuan Partridge Chickens from 1 to 30 Days

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