静原鸡ELOVL2基因遗传多态性研究

doi:10.16431/j.cnki.1671-7236.2018.03.022

摘要/Abstract

摘要：

为了探讨静原鸡极长链脂肪酸延伸酶蛋白2（elongation of very-long-chain fatty acids 2，ELOVL2）基因的遗传特性，确定核苷酸的变异位点，本研究采用PCR-SSCP及DNA测序技术对静原鸡ELOVL2基因进行多态性检测。结果显示，静原鸡ELOVL2基因外显子6无多态性，内含子2和6呈中度多态。在ELOVL2基因内含子2扩增片段上共检测到5种基因型：A₂A₂、B₂B₂、C₂C₂、D₂D₂和A₂C₂，4种等位基因：A₂、B₂、C₂和D₂，其中A₂为优势等位基因（0.62），A₂A₂基因型为优势基因型（0.54）；3个SNPs位点：g.63372228+4918G > A的转换、g.63372228+5024T > C的转换和g.63372228+4928C > A的颠换。在E2e6I6扩增片段上共检测到3种基因型：A₁A₁、B₁B₁和A₁B₁，2种等位基因：A₁和B₁，其中A₁为优势等位基因（0.67），A₁A₁基因型为优势基因型（0.54），等位基因B₁存在g.63388000+748G > C的颠换。群体遗传学分析发现，静原鸡ELOVL2基因内含子2、6遗传纯合度均较高，且偏离了Hardy-Weinberg平衡（P < 0.05）。本研究结果表明，静原鸡ELOVL2基因内含子2和6序列突变较高，且呈中度多态，表现出纯合子优势，外显子6上无突变。

关键词: 静原鸡; ELOVL2基因; 多态性

Abstract:

In order to study the genetic characteristics and nucleotide polymorphism sites of elongation of very-long-chain fatty acids 2 (ELOVL2) gene in Jingyuan chicken,the polymorphisms of ELOVL2 gene were detected by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and DNA sequence.The results showed that there was no polymorphism of ELOVL2 gene exon 6,there were moderate polymorphism of introns 2 and 6 in Jingyuan chicken.The total of 5 genotypes(A₂A₂,B₂B₂,C₂C₂,D₂D₂ and A₂C₂),4 alleles(A₂,B₂,C₂ and D₂) and 3 SNPs loci were detected on the intron 2 amplified fragment of ELOVL2 gene.The 3 SNPs were g.63372228+4918G > A transformation,g.63372228+5024T > C transformation and g.63372228+4928C > A transversion.A₂ was the dominant allele (0.62),and A₂A₂ was the dominant genotype (0.54).The total of 3 genotypes (A₁A₁,B₁B₁ and A₁B₁) and 2 alleles (A₁ and B₁) were detected on E2e6I6 amplified fragments.A₁ was the dominant allele (0.67),A₁A₁ was the dominant genotype (0.54),and only allele B₁ had a g.63388000+748G > C transversion.Population genetics analysis showed that genetic homozygosity of ELOVL2 gene introns 2 and 6 in Jingyuan chicken were higher and deviation from Hardy-Weinberg equilibrium (P < 0.05).The results showed that the mutations of the ELOVL2 gene introns 2 and 6 in the Jingyuan chicken were high,which were moderate polymorphism,showing the homozygous advantage and no mutation on the exon 6.

Key words: Jingyuan chicken; ELOVL2 gene; polymorphism

中图分类号:

S813.3

母童, 顾亚玲, 赵平, 冯小芳, 陈佳萍, 陈亚飞, 刘丽元, 蒋秋斐, 张娟. 静原鸡ELOVL2基因遗传多态性研究[J]. 《中国畜牧兽医》, 2018, 45(3): 730-737.

MU Tong, GU Yaling, ZHAO Ping, FENG Xiaofang, CHEN Jiaping, CHEN Yafei, LIU Liyuan, JIANG Qiufei, ZHANG Juan. Study on Polymorphism of ELOVL2 Gene in Jingyuan Chicken[J]. , 2018, 45(3): 730-737.

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