《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (6): 1778-1783.doi: 10.16431/j.cnki.1671-7236.2017.06.028

• 遗传繁育 • 上一篇    下一篇

绵羊MyoG基因外显子1的多态性及其与肉质性状的关联分析

白俊艳, 杨又兵, 王玉琴, 庞有志, 王旭, 杨帅, 王欢玲   

  1. 河南科技大学动物科技学院, 洛阳 471003
  • 收稿日期:2016-10-17 出版日期:2017-06-20 发布日期:2017-06-28
  • 通讯作者: 王玉琴 E-mail:wangyq6836@163.com
  • 作者简介:白俊艳(1975-),女,蒙古族,内蒙古赤峰人,博士,副教授,硕士生导师,研究方向:绵羊分子育种,E-mail:junyanbai@163.com
  • 基金资助:

    本研究由"十三五"国家现代肉羊产业技术体系(CARS-39);国家星火计划项目(2015GA750002)

Polymorphism of MyoG Gene Exon 1 and Its Association Analysis with Meat Quality Traits in Sheep

BAI Jun-yan, YANG You-bing, WANG Yu-qin, PANG You-zhi, WANG Xu, YANG Shuai, WANG Huan-ling   

  1. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China
  • Received:2016-10-17 Online:2017-06-20 Published:2017-06-28

摘要:

肌细胞生成素(myogenin,MyoG)基因在肌细胞分化过程中起着中心调节作用,直接影响着动物的产肉能力。本研究以大尾寒羊、小尾寒羊、豫西脂尾羊、兰州大尾羊、蒙古羊、同羊6个绵羊品种为试验材料,利用非变性聚丙烯酰胺凝胶电泳检测MyoG基因外显子1的多态性,并与绵羊的肉质性状进行关联分析,探讨MyoG基因外显子1对绵羊肉质性状的影响。结果表明,MyoG基因外显子1在6个绵羊群体中均检测到3种基因型(AA、BB、AB)和2个等位基因(A、B),在小尾寒羊、大尾寒羊、豫西脂尾羊、兰州大尾羊、蒙古羊、同羊群体中检测到MyoG基因外显子1的A等位基因频率分别为:0.5167、0.2500、0.4375、0.6500、0.5750和0.7125,MyoG基因外显子1的B等位基因频率分别为:0.4833、0.7500、0.5625、0.3500、0.4250和0.2875。MyoG基因外显子1主要对羊肉的水分和色泽有影响,主要表现为BB基因型水分极显著高于AB基因型(P< 0.01),显著高于AA基因型(P< 0.05);AB基因型的色泽极显著高于BB基因型(P< 0.01),显著高于AA基因型(P< 0.05)。

关键词: 绵羊; 肌细胞生成素基因; 肉质性状; 关联分析

Abstract:

Myogenin (MyoG) gene plays a central regulatory role in the process of muscle cell differentiation, which directly affects meat production capacity of animal. In this study, 6 sheep varieties including Large-tail Han sheep, Small-tail Han sheep, Yuxizhiwei sheep, Lanzhou fat-tailed sheep, Mongolia sheep and Tong sheep were selected as experimental materials to detect the polymorphism of MyoG gene exon 1 by non denaturing polyacrylamide gel electrophoresis, and analyze the association analysis between the polymorphism of MyoG gene exon 1 and meat quality traits in sheep. The results showed that 2 alleles (A, B) and 3 genotypes (AA, BB, AB) were detected in MyoG gene exon 1 of six sheep populations. The A allele frequency in Small-tail Han sheep, Large-tail Han sheep, Yuxizhiwei sheep, Lanzhou fat-tailed sheep, Mongolia sheep and Tong sheep were 0.5167, 0.2500, 0.4375, 0.6500, 0.5750 and 0.7125, respectively, the B allele frequency of six sheep populations were 0.4833, 0.7500, 0.5625, 0.3500, 0.4250 and 0.2875, respectively. MyoG gene exon 1 mainly affected the moisture and color of mutton, the moisture content of BB genotype was significantly higher than that of AB genotype (P< 0.01) and AA genotype (P< 0.05). The color of AB genotype was significantly higher than that of BB genotype (P< 0.01) and AA genotype (P< 0.05).

Key words: sheep; MyoG gene; meat quality traits; association analysis

中图分类号: