《中国畜牧兽医》 ›› 2016, Vol. 43 ›› Issue (10): 2621-2627.doi: 10.16431/j.cnki.1671-7236.2016.10.016

• 生理生化 • 上一篇    下一篇

转lncRNA-GTL2对小鼠肠道微生物菌群组成的影响

范新浩1,2, 王子帅2, 梁国明2,3, 杨亚岚2, 李奎2, 宋德贵1, 唐中林2,3   

  1. 1. 广西师范大学生命科学学院, 桂林 541000;
    2. 中国农业科学院北京畜牧兽医研究所, 北京 100193;
    3. 中国农业科学院深圳农业基因组研究所, 深圳 518120
  • 收稿日期:2016-04-13 出版日期:2016-10-20 发布日期:2016-10-28
  • 通讯作者: 宋德贵, 唐中林 E-mail:sondegui@163.com;Zhonglinqy_99@sina.com
  • 作者简介:范新浩(1990-),男,山东新泰人,硕士生,研究方向:农业微生物学,E-mail:fanxinhao7@163.com
  • 基金资助:

    国家自然科学基金(31171192);中国农业科学院科技创新工程项目(cxgc-ias-16)

Effects of lncRNA-GTL2 Transgenosis on Gut Microbiota Composition in Mice

FAN Xin-hao1,2, WANG Zi-shuai2, LIANG Guo-ming2,3, YANG Ya-lan2, LI Kui2, SONG De-gui1, TANG Zhong-lin2,3   

  1. 1. College of Life Science, Guangxi Normal University, Guilin 541000, China;
    2. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    3. Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China
  • Received:2016-04-13 Online:2016-10-20 Published:2016-10-28

摘要:

本试验旨在研究通过转基因技术转入长链非编码RNA基因是否会对小鼠肠道微生物的菌落结构和构成产生影响,利用高通量测序技术对3月龄转基因和非转基因小鼠的肠道微生物菌群进行测序和分析,在门水平和属水平进行对比和t检验,结果显示,在同一性别内转入长链非编码RNA基因GTL2(lncRNA-GTL2)后,小鼠肠道微生物的菌落结构和构成总体上没有显著差异,但存在个体差异。本研究未发现转入长非编码RNA对肠道微生物菌群在门和属水平上产生显著的影响。

关键词: 转基因; lncRNA-GTL2; 高通量测序; 肠道微生物

Abstract:

This research was aimed to study whether the lncRNA would have effects on the structure and constitution of the intestinal microbial colonies in mice.High throughout sequencing was used to sequence and analyze the intestinal microbial colonies in both transgenic and non-transgenic mice of three months old.We conducted comparison and t-test at the level of phylum and genus.The result showed that transferred into long non-coding RNA genes GTL2 (lncRNA-GTL2),the mouse intestinal microbial colony structure and composition had no significant differences in the overall,within the same gender,but there were individual differences.Therefore,this study didn't find that long non-coding RNA transfered had a significant impact on the intestinal microflorain the phylum and genus level.

Key words: transgenic; lncRNA-GTL2; high-throughput sequencing; intestinal microorganisms

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