环江香猪黏附素13基因多态性及其与仔猪腹泻的相关性分析

doi:10.16431/j.cnki.1671-7236.2016.07.025

《中国畜牧兽医》---唯一指定的官方网站 ›› 2016, Vol. 43 ›› Issue (7): 1818-1825.doi: 10.16431/j.cnki.1671-7236.2016.07.025

环江香猪黏附素13基因多态性及其与仔猪腹泻的相关性分析

李俊¹, 许钟峯¹, 张海航¹, 尚月丽¹, 孔祥峰², 石德顺¹, 刘庆友¹

1. 广西大学, 亚热带生物资源保护与利用国家重点实验室, 南宁 530004;
2. 中国科学院亚热带农业生态研究所, 长沙 410125

收稿日期:2015-12-21 出版日期:2016-07-20 发布日期:2016-07-22
通讯作者: 石德顺, 刘庆友 E-mail:ardsshi@gxu.edu.cn;qyliu2002@126.com
作者简介:李俊(1990-),男,云南大理人,硕士生,研究方向:动物繁殖育种,E-mail:1165171579@qq.com
基金资助:
国家高技术研究发展计划（863）项目（2013AA102504）；国家自然基金项目（31260552）

Association Analysis of MUC13 Gene Polymorphisms with Diarrhea in Huanjiang Miniature Pigs

LI Jun¹, XU Zhong-feng¹, ZHANG Hai-hang¹, SHANG Yue-li¹, KONG Xiang-feng², SHI De-shun¹, LIU Qing-you¹

1. State Key Laboratory of Tropical Biological Resources Protection and Utilization of Nanning, Guangxi University, Nanning 530004, China;
2. Institute of Subtropical Agriculture Chinese Academy of Sciences, Changsha 410125, China

Received:2015-12-21 Online:2016-07-20 Published:2016-07-22

摘要/Abstract

摘要：

试验旨在对环江香猪仔猪腹泻抗性/易感的黏附素13基因（MUC13）单核苷酸多态性（single nucleotide polymorphism，SNP）/单倍型与仔猪腹泻的相关性进行分析。试验采用高分辨率熔解曲线（high resolution melting，HRM）技术对93头环江香猪腹泻仔猪和95头健康对照仔猪进行基因分型，结合DNA测序对HRM检测结果进行验证，并通过SHEsis在线平台确定其单倍型。结果显示，环江香猪MUC13基因的G224A位等位基因分布频率在腹泻组和对照组间差异显著（P < 0.05），GG、GA和GG+GA基因型分别与AA基因型相比差异均显著（P < 0.05）。A112G、T170C和G164C位点各基因型频率和等位基因频率在腹泻组和对照组之间差异均不显著（P > 0.05），腹泻组与对照组E位点均为野生纯合基因型。单倍型Ⅹ（GTAC）在腹泻组的分布极显著高于对照组（P < 0.01）。因此，MUC13基因G224A位点和单倍型Ⅹ（GTAC）与环江香猪仔猪断奶前腹泻相关。

关键词: 环江香猪; 黏附素13基因; 单核苷酸多态性; 单倍型; 仔猪腹泻

Abstract:

The purpose of the study was to analyze the association between MUC13 gene polymorphisms and piglet diarrhea in Huanjiang miniature pigs.93 diarrhea piglets and 95 healthy controls were included in the study.The MUC13 gene polymorphisms were determined using high resolution melting (HRM),and the results of HRM were verified by direct sequencing.Mutations were compared between diarrhea piglets and healthy controls.SHEsis online platform was used to calculate the haplotypes.There were statistically significant association on G224A site showed significant difference between diarrhea piglets and healthy controls by allele frequencies (P < 0.05),and there were also statistically significant differences between the two groups by genotypes among GG,GA,GG+GA and AA (P < 0.05).But no statistically significant association was found between polymorphisms of A112G,T170C and G164C sites and piglet diarrhea risk (P > 0.05).However,haplotype Ⅹ (GTAC) in the distribution of diarrhea group was extremely significantly higher (P < 0.01).The present data revealed that MUC13 gene G224A site and the haplotypes Ⅹ (GTAC) were associated with reduce risk of piglet diarrhea.

Key words: Huanjiang miniature pigs; MUC13 gene; SNP; haplotypes; piglet diarrhea

中图分类号:

S813.3

李俊, 许钟峯, 张海航, 尚月丽, 孔祥峰, 石德顺, 刘庆友. 环江香猪黏附素13基因多态性及其与仔猪腹泻的相关性分析[J]. 《中国畜牧兽医》---唯一指定的官方网站, 2016, 43(7): 1818-1825.

LI Jun, XU Zhong-feng, ZHANG Hai-hang, SHANG Yue-li, KONG Xiang-feng, SHI De-shun, LIU Qing-you. Association Analysis of MUC13 Gene Polymorphisms with Diarrhea in Huanjiang Miniature Pigs[J]. , 2016, 43(7): 1818-1825.

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环江香猪黏附素13基因多态性及其与仔猪腹泻的相关性分析

Association Analysis of MUC13 Gene Polymorphisms with Diarrhea in Huanjiang Miniature Pigs

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