PRDM家族蛋白结构与功能研究进展

doi:10.16431/j.cnki.1671-7236.2016.05.010

摘要/Abstract

摘要： PRDM家族蛋白是具有一个PR结构域和数量不等锌指结构(除了PRDM11),广泛存在于灵长类、啮齿动物、鸟类和两栖动物中能够调控表观遗传的蛋白。部分PRDM蛋白家族成员的PR结构域具有组蛋白赖氨酸转移酶活性(HMT),影响表观遗传,PRDM蛋白锌指结构具有DNA、RNA、蛋白识别结合能力;另一部分PRDM家族成员虽然具有PR结构域,但不具有HMT活性,在癌症发生、原始生殖细胞特化、神经系统发育、造血、血管发展、胚胎发育中发挥重要作用。PRDM家族蛋白通过甲基转移或募集染色体结构重塑复合物,调控基因表达和修改染色体结构,在细胞特化和疾病调控中起到重要作用。论文综述了PRDM家族蛋白结构与功能最新研究进展。

关键词: PRDM家族蛋白; 表观遗传; PR结构域; 锌指结构; 组蛋白赖氨酸转移酶活性

Abstract: PRDM protein family which has a PR domain and varying amounts of zinc finger domains (except PRDM11), widely present in primates, rodents, birds and amphibians, involve in regulation of epigenetic proteins.Some PR domains possess transferase activity of histone lysine (HMT), affect epigenetics features and the Zinc finger domains may recognize and bind DNA, RNA, protein;The other PR domains of PRDM without HMT activity, play a key role in carcinogenesis, specification of primordial germ cell, neurogenesis, hematopoiesis, vascular development, and embryonic development.PRDM protein family participate in cell specialization, and disease regulation, regulating gene expression and modifying chromatin structure by methyl transfer, or recruited chromosome structure reshape compounds.This review summarized the latest research progress on structure and function of PRDM protein family.

Key words: PRDM protein family; epigenetics; PR domain; zinc finger; histone lysine transferase activity

中图分类号:

赵生军, 张勇, 阎萍, 郭宪. PRDM家族蛋白结构与功能研究进展[J]. , 2016, 43(5): 1188-1193.

ZHAO Sheng-jun, ZHANG Yong, YAN Ping, GUO Xian. Advances on Structure and Function of PRDM Protein Family[J]. , 2016, 43(5): 1188-1193.

参考文献

[1] Fog C K,Galli G G,Lund A H.PRDM proteins:Important players in differentiation and disease[J].Bioessays,2012,34(1):50-60.
[2] Fumasoni I,Meani N,Rambaldi D,et al.Family expansion and gene rearrangements contributed to the functional specialization of PRDM genes in vertebrates[J].BMC Evol Biol,2007,7(7):187.
[3] Di Z E,De R C,Abbondanza C,et al.PRDM proteins:Molecular mechanisms in signal transduction and transcriptional regulation[J].Biology,2013,2(1):107-141.
[4] 李瑶,呼群.表观遗传调控与肿瘤耐药[J]. 现代肿瘤医学,2016,24(2):322-325.
[5] Qian C,Zhou M M.SET domain protein lysine methyltransferases:Structure,specificity and catalysis[J].Cellular and Molecular Life Sciences,2006,63(23):2755-2763.
[6] 薛红.SET基因家族研究进展[J].医学分生物学杂志,2005,2(3):190-193.
[7] Herz H M,Garruss A,Shilatifard A.SET for life:Biochemical activities and biological functions of SET domain-containing proteins[J].Trends Biochem Sci,2013,38(12):621-639.
[8] Lanouette S,Mongeon V,Figeys D,et al.The functional diversity of protein lysine methylation[J].Molecular Systems Biology,2014,10(4):168-171.
[9] Du T T.The roles of histone lysine methylation in epigenetic regulation[J].Hereditas,2007,29(4):387-392.
[10] Jackson J P,Johnson L,Jasencakova Z,et al.,Dimethylation of histone H3 lysine 9 is a critical mark for DNA methylation and gene silencing in arabidopsis thaliana[J].Chromosoma,2004,112(6):308-315.
[11] Shao G B,Chen J C,Zhang L P,et al.Dynamic patterns of histone H3 lysine 4 methyltransferases and demethylases during mouse preimplantation development[J].In Vitro Cell Dev Biol Anim,2014,50(7):603-613.
[12] Choi J Y,Lee S,Hwang S,et al.Histone H3 lysine 27 and 9 hypermethylation within the Bad promoter region mediates 5-Aza-2'-deoxycytidine-induced leydig cell apoptosis:Implications of 5-Aza-2'-deoxycytidine toxicity to male reproduction[J].Apoptosis,2013,18(1):99-109.
[13] Pham K T M,Nguyen H H,Murai T,et al.Histone H3K4 methyltransferase globally regulates substrate-dependent activation of cell-wall-degrading enzymes in magnaporthe oryzae[J].Journal of General Plant Pathology,2014,81(2):127-130.
[14] Xiao B,Wilson J R,Gamblin S J.SET domains and histone methylation[J].Current Opinion in Structural Biology,2003,13(6):699-705.
[15] Veerappan C S,Avramova Z,Moriyama E N.Evolution of SET-domain protein families in the unicellular and multicellular Ascomycota fungi[J].BMC Evol Biol,2008,8(3):190.
[16] Corkins M E,Margot M,Ehrensberger K M,et al.Zinc finger protein Loz1 is required for zinc-responsive regulation of gene expression in fission yeast[J].Proc Natl Acad Sci USA,2013,110(38):15371-15376.
[17] Ogo O A,John T,Cockell S J,et al.The zinc finger protein ZNF658 regulates the transcription of genes involved in zinc homeostasis and affects ribosome biogenesis through the zinc transcriptional regulatory element[J].Mol Cell Biol,2015,35(6):977-987.
[18] Yang Z,Wen H J,Minhas V,et al.The zinc finger DNA-binding domain of K-RBP plays an important role in regulating Kaposi's sarcoma-associated herpesvirus RTA-mediated gene expression[J].Virology,2009,391(2):221-231.
[19] 李晓波,张俊武.真核生物中锌指蛋白的结构与功能[J].中国生物化学与分子生物学报,2009,25(3):206-211.
[20] Hohenauer T,Moore A W.The PRDM family:Expanding roles in stem cells and development[J].Development,2012,139(13):2267-2282.
[21] Buyse I M,Huang S.In vitro analysis of the E1A-homologous sequences of RIZ[J].J Virol,1997,71(8):6200-6203.
[22] Liu L,Shao G,Steele-Perkins G,et al.The retinoblastoma interacting zinc finger gene RIZ produces a PR domain-lacking product through an internal promoter[J].J Biol Chem,1997,272(5):2984-2991.
[23] Cheedipudi S,Puri D,Saleh A,et al.A fine balance:Epigenetic control of cellular quiescence by the tumor suppressor PRDM2/RIZ at a bivalent domain in the cyclin a gene[J].Nucleic Acids Res,2015.43(13):6236-6256.
[24] Du Y,Carling T,Fang W,et al.Hypermethylation in human cancers of the RIZ1 tumor suppressor gene,a member of a histone/protein methyltransferase superfamily[J].Cancer Res,2001,61(22):8094-8099.
[25] Sasaki O,Meguro K,Tohmiya Y,et al.Altered expression of retinoblastoma protein-interacting zinc finger gene,RIZ,in human leukaemia[J].Br J Haematol,2002,119(4):940-948.
[26] Lal G,Padmanabha L,Smith BJ,et al.RIZ1 is epigenetically inactivated by promoter hypermethylation in thyroid carcinoma[J].Cancer,2006,107(12):2752-2759.
[27] Gao H,Wang F,Lan X,et al.Lower PRDM2 expression is associated with dopamine-agonist 272 resistance and tumor recurrence in prolactinomas[J].BMC Cancer,2015,15:272.
[28] Eom G H,Kim K,Kim S M,et al.Histone methyltransferase PRDM8 regulates mouse testis steroidogenesis[J].Biochem Biophys Res Commun,2009,388(1):131-136.
[29] Komai T,Iwanari H,Mochizuki Y,et al.Expression of the mouse PR domain protein PRDM8 in the developing central nervous system[J].Gene Expr Patterns,2009,9(7):503-514.
[30] Gu B,Lee M G.Histone H3 lysine 4 methyltransferases and demethylases in self-renewal and differentiation of stem cells[J].Cell & Bioscience,2013,3:39.
[31] Eram M S,Bustos S P,Lima-Fernandes E,et al.Trimethylation of histone H3 lysine 36 by human methyltransferase PRDM9 protein[J].Journal of Biological Chemistry,2014,289(17):12177-12188.
[32] Schmidt D,Nayak A,Julia E S,et al.Blimp-1 exon7:A naturally occurring Blimp-1 deletion mutant with auto-regulatory potential[J].Experimental Cell Research,2008,314(20):3614-3627.
[33] Gyory I,Wu J,Fejer G,et al.PRDI-BF1 recruits the histone H3 methyltransferase G9a in transcriptional silencing[J].Nat Immunol,2004,5(3):299-308.
[34] D'Costa K,Emslie D,Metcalf D,et al.Blimp1 is limiting for transformation in a mouse plasmacytoma model[J].Blood,2009.113(23):5911-5919.
[35] Cheng H Y,Chen X W,Cheng L,et al.DNA methylation and carcinogenesis of PRDM5 in cervical cancer[J].J Cancer Res Clin Oncol,2010,136(12):1821-1825.
[36] Zhang T,Meng L,Dong W,et al.High expression of PRDM14 correlates with cell differentiation and is a novel prognostic marker in resected non-small cell lung cancer[J].Med Oncol,2013,30(3):351-355.
[37] 郭晓强,王晓红.PR结构域蛋白质与原始生殖细胞特化[J].生命的化学,2010,30(1):4-7.
[38] Hirai H.The transcription factor Evi-1[J].International Journal of Biochemistry and Cell Biology,1999,31(12):1367-1371.
[39] Pinheiro I,Margueron R,Shukeir N,et al.PRDM3 and PRDM16 are H3K9me1 methyltransferases required for mammalian heterochromatin integrity[J].Cell,2012,150(5):948-960.
[40] Reid A G,Nacheva E P.A potential role for PRDM12 in the pathogenesis of chronic myeloid leukaemia with derivative chromosome 9 deletion[J].Leukemia,2004,18(1):178-180.
[41] 冀斌,孙亭亭,陈培富.细胞分化的甲基化调控及其分子机理[J].中国畜牧兽医,2014,41(3):203-206.