犬细小病毒的分离鉴定及其VP2基因序列分析

doi:10.16431/j.cnki.1671-7236.2015.10.011

›› 2015, Vol. 42 ›› Issue (10): 2587-2593.doi: 10.16431/j.cnki.1671-7236.2015.10.011

犬细小病毒的分离鉴定及其VP2基因序列分析

林鹏, 赵航, 王建科, 程悦宁, 任静强, 易立, 仝明薇, 程世鹏

中国农业科学院特产研究所, 吉林省特种经济动物分子生物学重点实验室, 长春 130112

收稿日期:2015-03-12 出版日期:2015-10-20 发布日期:2015-10-23
通讯作者: 程世鹏 E-mail:tcscsp@126.com
作者简介:林鹏(1990-),男,山东牟平人,硕士生,研究方向:动物病毒分子生物学,E-mail:lphs1002@163.com
基金资助:
吉林省自然科学基金项目(20140101029JC);吉林省重点科技攻关项目(20150204021NY);吉林省特种经济动物生物制品科技创新中心;中国农业科学院科技创新工程(CAAS-ASTIP-2014-ISAPS)

Isolation and Identification of Canine Parvovirus Virus and Sequence Analysis of VP2 Gene

LIN Peng, ZHAO Hang, WANG Jian-ke, CHENG Yue-ning, REN Jing-qiang, YI Li, TONG Ming-wei, CHENG Shi-peng

Jilin Provincial Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Changchun 130112, China

Received:2015-03-12 Online:2015-10-20 Published:2015-10-23

摘要/Abstract

摘要： 本试验从北京某动物医院疑似犬细小病毒(CPV)感染犬粪便中分离出一株病毒。通过电子显微镜观察、血凝试验、动物回归试验和分子生物学鉴定,本试验成功分离出一株CPV,命名为BJ-24。对BJ-24株的VP2基因序列分析发现该分离株为CPV-2a亚型,其VP2基因与GenBank中19株CPV VP2基因核苷酸序列有较高的同源性,均在98.7%以上,甚至与CPV-JS2株达到100.0%。系统进化分析结果表明所分离的BJ-24株属于中国分支,与CPV-JS2株遗传距离最近。本试验对进一步开展北京地区CPV的流行病学调查提供基础依据,并对防制病毒传播和疫苗研制奠定了坚实的基础。

关键词: 犬细小病毒; 分离; 鉴定; 序列分析

Abstract: A strain of virus was isolated and identified from feces of a suspected canine parvovirus (CPV) infected dog.It was eventually identified as CPV using electron microscope examination,hemagglutination (HA) test,animal regression experiment and molecular biology idenfication,designated as BJ-24 strain.The VP2 gene sequence analysis of BJ-24 strain showed that BJ-24 strain belonged to CPV-2a subtype.The analysis of VP2 gene indicated that the BJ-24 VP2 gene shared higher homology of nucleotide with 19 CPV strains available in GenBank and were more than 98.7%.The highest homology was 100.0% with CPV-JS2 strain.Phylogenetic tree analysis showed that BJ-24 strain belonged to China cluster and there was the closest distant between BJ-24 strain and CPV-JS2 strain.This study provided the basic evidence for further study on molecular epidemiology in Beijing and laid the solid foundation for developing vaccine and preventing the spread of CPV.

Key words: canine parvovirus; isolation; identification; sequence analysis

中图分类号:

林鹏, 赵航, 王建科, 程悦宁, 任静强, 易立, 仝明薇, 程世鹏. 犬细小病毒的分离鉴定及其VP2基因序列分析[J]. , 2015, 42(10): 2587-2593.

LIN Peng, ZHAO Hang, WANG Jian-ke, CHENG Yue-ning, REN Jing-qiang, YI Li, TONG Ming-wei, CHENG Shi-peng. Isolation and Identification of Canine Parvovirus Virus and Sequence Analysis of VP2 Gene[J]. , 2015, 42(10): 2587-2593.

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犬细小病毒的分离鉴定及其VP2基因序列分析

Isolation and Identification of Canine Parvovirus Virus and Sequence Analysis of VP2 Gene

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