褪黑素对羊肉抗氧化、肉品质及货架期的影响

doi:10.16431/j.cnki.1671-7236.2025.01.013

摘要/Abstract

摘要： 【目的】研究褪黑素(melatonin,MT)对羊肉抗氧化能力和肉品质的影响,探究MT在体外条件下是否具有改善肉品质和延长货架期的能力。【方法】选取体重相近(36.23 kg±0.31 kg)的5月龄湖羊,采集背最长肌分割后随机分为4组,对照组羊肉浸泡在蒸馏水中,试验Ⅰ、Ⅱ、Ⅲ组羊肉分别浸泡在含3、6和9 ng/mL MT的溶液中,30 min后沥干装入自封袋4 ℃贮存,在试验第1天对其MT含量进行测定,在试验第1、4、7、10和13天对其抗氧化能力和肉品质进行测定,并在试验12、36、84、132、180、228 h测定羊肉中挥发性盐基氮(total volatile base nitrogen,TVB-N)含量。【结果】与对照组相比,试验Ⅰ、Ⅱ和Ⅲ组羊肉MT含量极显著升高(P＜0.01);在试验第1、4、7天试验Ⅲ组羊肉总抗氧化能力(T-AOC)显著升高(P＜0.05),试验Ⅱ、Ⅲ组羊肉丙二醛(MDA)含量极显著降低(P＜0.01);在试验第7、10、13天试验Ⅱ、Ⅲ组羊肉pH显著降低(P＜0.05),试验Ⅰ组羊肉滴水损失极显著降低(P＜0.01),试验Ⅰ、Ⅱ和Ⅲ组羊肉货架期分别延长9.11、12.89、14.91 h。【结论】 MT在体外具有一定提高羊肉抗氧化和改善肉品质的能力,且可以延长羊肉货架期,建议添加浓度为3 ng/mL。

关键词: 褪黑素; 抗氧化; 货架期; 肉品质

Abstract: 【Objective】 This experiment was aimed to study the effect of melatonin (MT) on the antioxidant capacity and quality of mutton,and investigate whether or not MT could improve the quality of mutton and extend shelf life in vitro. 【Method】 Five-month-old Hu sheep with similar body weight (36.23 kg±0.31 kg) were selected,and the longissimus dorsi muscle was collected and randomly divided into four groups.The mutton in control group was soaked in distilled water,and the mutton in experiment groups Ⅰ,Ⅱ and Ⅲ were soaked in the solution containing 3,6 and 9 ng/mL MT,respectively.After 30 min,it was drained and stored in a self-sealing bag at 4 ℃.The MT content was measured on the first day of the experiment.The antioxidant capacity and meat quality were measured on the 1st,4th,7th,10th and 13th days of the experiment.The total volatile base nitrogen (TVB-N) content in mutton was measured at 12,36,84,132,180 and 228 h of the experiment. 【Result】 Compared with control group,the MT contents of mutton in groups Ⅰ,Ⅱ and Ⅲ were extremely significantly increased (P＜0.01).On the 1st,4th and 7th days of the experiment,the total antioxidant capacity (T-AOC) of mutton in group Ⅲ was significantly increased (P＜0.05),the malondialdehyde (MDA) contents of mutton in groups Ⅱ and Ⅲ were extremely significantly decreased (P＜0.01).On the 7th,10th and 13th days of the experiment,pH of mutton in groups Ⅱ and Ⅲ was significantly decreased (P＜0.05),the drip loss of mutton in group Ⅰ was extremely significantly decreased (P＜0.01),the shelf life of mutton in groups Ⅰ,Ⅱ and Ⅲ was extended by 9.11,12.89 and 14.91 h,respectively. 【Conclusion】 MT could improve the antioxidant capacity and quality of mutton in vitro to a certain degree,and extend the shelf life of mutton.In summary,this study recommended 3 ng/mL as the optimal concentration of MT.

Key words: melatonin; antioxidant; shelf life; meat quality

中图分类号:

S879.2

许世恒, 范文鹏, 阮俞乔, 祖丽比娜·艾尼瓦尔, 马晓翠, 罗琦雨, 徐同翔, 王彩蝶. 褪黑素对羊肉抗氧化、肉品质及货架期的影响[J]. 中国畜牧兽医, 2025, 52(1): 147-157.

XU Shiheng, FAN Wenpeng, RUAN Yuqiao, Zulibina Ainiwaer, MA Xiaocui, LUO Qiyu, XU Tongxiang, WANG Caidie. Effect of Melatonin on Antioxidant,Meat Quality and Shelf Life of Mutton[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(1): 147-157.

参考文献

[1] ABECIA J,FORCADA F,VAZQURZ M,et al.Role of melatonin on embryo viability in sheep[J].Reproduction,Fertility and Development,2019,31(1):82-92.
[2] YANG C H,WU Z Y,LI Y,et al.Effect of melatonin administration to lactating cashmere goats on milk production of dams and on hair follicle development in their offspring[J].Animal,2020,14(6):1241-1248.
[3] 苑志宇,赵云辉,王嵩,等.外源褪黑素对羔羊生长发育的影响[J].黑龙江畜牧兽医,2022,22:53-57. YUAN Z Y,ZHAO Y H,WANG S,et al.Effects of exogenous melatonin on the growth and development of lambs[J].Heilongjiang Animal Science and Veterinary Medicine,2022,22:53-57.(in Chinese)
[4] 张嗣禹,崔亚芳,邱鑫君,等.褪黑素对机体抗氧化能力和脂质代谢的影响及作用机制[J].中国畜牧杂志,2023,59(6):1-9. ZHANG S Y,CUI Y F,QIU X J,et al.Effects and mechanism of melatonin on body antioxidant capacity and lipid metabolism[J]. Chinese Journal of Animal Science,2023,59(6):1-9.(in Chinese)
[5] 代国杰,朱建津,高琳琳,等.褪黑素对小鼠抗氧化作用的影响[J].食品工业科技,2010,31(3):347-350. DAI G J,ZHU J J,GAO L L,et al.Effect of melatonin on the antioxidation of mice[J]. Science and Technology of Food Industry,2010,31(3):347-350.(in Chinese)
[6] 张全江,李秋霞,熊正英,等.连续51天服用褪黑激素对小鼠肾脏、心肌与脑部分生化指标及体重变化的影响[J].食品科学,2003,4:142-145. ZHANG Q J,LI Q X,XIONG Z Y,et al.Effects of melatonin administration for 51 consecutive days on the biochemical indexes and body weight changes of kidney,myocardium and brain in mice[J]. Food Science,2003,4:142-145.(in Chinese)
[7] 曹旭阳,黄敏,杨汉文,等.褪黑素对奶山羊精液低温冷藏的影响[J].中国兽医学报,2023,43(7):1520-1527. CAO X Y,HUANG M,YANG H W,et al.Effects of melatonin oncooled-storage of dairy goat semen[J].Chinese Journal of Veterinary Science,2023,43(7):1520-1527.(in Chinese)
[8] 康凌云,韩露露,韩德平,等.褪黑素缓解空肠黏膜上皮细胞氧化损伤的效果研究[J].生物技术通报,2023,39(9):291-299. KANG L Y,HAN L L,HAN D P,et al.Effect of melatonin on protecting the jejunum mucosal epithelial cells from oxidative stress damage[J].Biotechnology Bulletin,2023,39(9):291-299.(in Chinese)
[9] 樊路杰,窦鸣乐,王小宇,等.宰后肌肉抗氧化能力与肉品质的关系[J].动物营养学报,2018,30(5):1676-1680. FAN L J,DOU M L,WANG X Y,et al.Relationship between antioxidant capacity of postmortem muscle and meat quality[J].Chinese Journal of Animal Nutrition,2018,30(5):1676-1680.(in Chinese)
[10] FALOWO A B,FAYEMI P O,MUCHENJ V.Natural antioxidants against lipid-protein oxidative deterioration in meat and meat products:A review[J].Food Research International,2014,64:171-181.
[11] 雪雅妮.饲粮能量水平对高原型藏羊生长性能、血清生化指标及肉品质的影响[J].饲料工业,2024,45(5):64-69. XUE Y N.Effects of dietary energy levels on growth performance,serum biochemical indicators,and meat quality of plateau Tibetan sheep[J].Feed Industry,2024,45(5):64-69.(in Chinese)
[12] 李书仪.沙葱及其提取物对肉羊血清抗氧化指标、羊肉品质及货架期的影响[D].呼和浩特:内蒙古农业大学,2021. LI S Y.Effects of allium mongolicum regel and its extracts on antioxidant indexes of serum,mutton quality and shelf life in lamb[D].Hohhot:Inner Mongolia Agricultural University,2021.(in Chinese)
[13] 吴菊清,李春保,周光宏,等.宰后成熟过程中冷却牛肉、猪肉色泽和嫩度的变化[J].食品科学,2008,10:136-139. WU J Q,LI C B,ZHOU G H,et al.Changes of meat color and tenderness of chilled beef and pork during postmortem aging[J].Food Science,2008,10:136-139.(in Chinese)
[14] 张华强,王品胜,李晓,等.发酵杂交构树对湖羊生长性能、肉品质、消化酶活力及肠道菌群结构的影响[J].中国畜牧兽医,2023,50(5):1876-1887. ZHANG H Q,WANG P S,LI X,et al.Effects of fermented hybrid Broussonetia papyrifera on growth performance,meat quality,digestive enzyme activity and intestinal flora structure of Hu sheep[J].China Animal Husbandry & Veterinary Medicine,2023,50(5):1876-1887.(in Chinese)
[15] 丁鑫.不同栗木单宁水平日粮对湖羊公羔生产性能和肉品质的影响[D].兰州:兰州大学,2019. DING X.Effects of different chestnut tannin levels on the production performance and meat quality of Hu lambs[D].Lanzhou:Lanzhou University,2019.(in Chinese)
[16] PEZESHK S,REZAEI M,HOSSEINI H.Effects of turmeric,shallot extracts,and their combination on quality characteristics of vacuum-packaged rainbow trout stored at 4 ℃±1 ℃[J].Journal of Food Science,2011,76(6):387-391.
[17] TAN D X,ZANGHI B M,MANCHESTER L C,et al.Melatonin identified in meats and other food stuffs:Potentially nutritional impact[J]. Journal of Pineal Research,2014,57(2):213-218.
[18] LOBO L L,ClAUSTRAT B,DEBILLY G,et al.Hypoprolactinemic rats under conditions of constant darkness or constant light.Effects on the sleep-wake cycle,cerebral temperature and sulfatoxymelatonin levels[J].Brain Research,1999,835(2):282-289.
[19] 卢玉发,廖清华.褪黑素对肉鸡生长性能及免疫功能的影响[J].饲料工业,2008,13:37-39. LU Y F,LIAO Q H.Influence of melatonin on growth performance and immune functions of broiler[J].Feed Industry,2008,13:37-39.(in Chinese)
[20] 向妙莲,吴帆,李树成,等.褪黑素处理对梨果实采后黑斑病及贮藏品质的影响[J].中国农业科学,2022,55(4):785-795. XIANG M L,WU F,LI S C,et al.Effects of melatonin treatment on resistance to black spot and postharvest storage quality of pear fruit[J].Scientia Agricultura Sinica,2022,55(4):785-795.(in Chinese)
[21] CAI S J,ZHANG Z Q,WANG J L,et al.Effect of exogenous melatonin on postharvest storage quality of passion fruit through antioxidant metabolism[J].Food Science,2024.Doi:10.1016/j.lwt.2024.115835.
[22] REITER R J,MANCHESTER L C,TAN D X.Melatonin in walnuts:Influence on levels of melatonin and total antioxidant capacity of blood[J].Nutrition,2005,21(9):920-924.
[23] 潘军. 十二指肠灌注5-羟基色氨酸对绵羊胃肠道、循环血液褪黑素水平的影响[D].乌鲁木齐:新疆农业大学, 2021. PAN J. Effects of duodenal 5-hydroxytryptophan perfusion on melatonin levels of gastrointestinal track and blood circulation in sheep[D]. Urumqi: Xinjiang Agricultural University, 2021. (in Chinese)
[24] 周亚星.褪黑素原位凝胶缓释剂的优化设计及肉鸡体内评价[D].郑州:河南农业大学,2023. ZHOU Y X.Optimal design of in situ gel sustained release agent for melation and evaluation in vivo of broilers[D].Zhengzhou:Henan Agricultural University,2023.(in Chinese)
[25] 张文杰,韩明,王世昌,等.补喂色氨酸对伊犁马血浆5-羟色胺、褪黑素含量及抗氧化能力的影响[J].中国畜牧杂志,2020,56(5):132-135. ZHANG W J,HAN M,WANG S C,et al.Effects of tryptophan supplementation on plasma trytophan concentrations,melatonin content and antioxidant indices in Yili horse[J].Chinese Journal of Animal Science,2020,56(5):132-135.(in Chinese)
[26] 周扬.褪黑素对热应激种公羊精液品质影响作用机理的分析[D].太谷:山西农业大学,2022. ZHOU Y.Mechanism analysis of melatonin effects on semen quality of heat stressed ram[D].Taigu:Shanxi Agricultural University,2022.(in Chinese)
[27] KUMAR A,MEHROTRA S,SINGH G,et al.Sustained delivery of exogenous melatonin influences biomarkers of oxidative stress and total antioxidant capacity in summer-stressed anestrous water buffalo (Bubalus bubalis)[J].Theriogenology,2015,83(9):1402-1407.
[28] KANYAR I M,KARADAS F.Effict of melatonin and vitamin E as antioxidants on body weight,carcass traits of Awassi lambs fed a high-energy and normal diet[J].The Iraqi Journal of Agricultural Science,2023,54(5):1339-1350.
[29] SABBAGHI V,MEHRI M,MEHRI M.Response surface model to illustrate the benefits of tryptophan,melatonin,and N,N-dimethylglycine in quail chicks exposed to aflatoxin B₁[J].Poultry Science,2023,102(8):102803.
[30] MANCINI R A,HUNT M C.Current research in meat color[J].Meat Science,2005,71(1):100-121.
[31] YU Q,COOPER B,SOBREIRA T,et al.Utilizing pork exudate metabolomics to reveal the impact of aging on meat quality[J].Foods,2021,10(3):668.
[32] 陈松,冯月荣,曹淑萍.pH值对屠宰肉品质的影响[J].肉类工业,2009,6:21-23. CHEN S,FENG Y R,CAO S P.Effects of pH value on quality of slaughter meat[J].Meat Industry,2009,6:21-23.(in Chinese)
[33] 杨文军,牟春堂,王鹏举,等.饲粮中添加葡萄籽原花青素对羔羊生长性能、屠宰性能、肉品质及血清抗氧化指标的影响[J].动物营养学报,2020,32(6):2755-2764. YANG W J,MU C T,WANG P J,et al.Effects of dietary supplementation of grape seed proanthocyandins on growth performance,slaughter performance,meat quality and serum antioxidant indexes of lambs[J].Chinese Journal of Animal Nutrition,2020,32(6):2755-2764.(in Chinese)
[34] 臧传云,崔凯,高惠,等.埋植褪黑激素对蓝狐肌肉品质及褪黑激素含量的影响[J].黑龙江畜牧兽医,2022,12:110-114. ZANG C Y,CUI K,GAO H,et al.Effects of melatonin implanted on muscle quality and melatonin content of blue foxes[J].Heilongjiang Animal Science and Veterinary Medicine,2022,12:110-114.(in Chinese)
[35] 彭焕伟.饲喂褪黑激素对生长肥育猪生产性能、胴体品质及肉质的影响[D].雅安:四川农业大学,2006. PENG H W.Effects of oral melatonin on performance,carcass and meat quality for growing-finishing pigs[D].Ya’an:Sichuan Agricultural University,2006.(in Chinese)
[36] CHEN Y,PENG L L,YI H,et al.Effect of dietary supplementation with melatonin on meat quality,fatty acid profiles and metabolome differences in broiler breast[J].Journal of Food Composition and Analysis,2023,121:105410.
[37] REITER R J,MAYO J C,TAN D X,et al.Melatonin as an antioxidant:Under promises but over delivers[J].Journal of Pineal Research,2016,61(3):253-278.
[38] RODRIGUER C,MAYO J C,SAINZ R M,et al.Regulation of antioxidant enzymes:A significant role for melatonin[J]. Journal of Pineal Research,2010,36(1):1-9.
[39] CASTER P,PADRON J C P,CANSINO M J C,et al.Total volatile base nitrogen and its use to assess freshness in European sea bass stored in ice[J].Food Control,2006,17(4):245-248.
[40] DONDERO M,CISTERNAS F,CARVAJAL L,et al.Changes in quality of vacuum-packed cold-smoked salmon (Salmo salar) as a function of storage temperature[J].Food Chemistry,2004,87(4):543-550.
[41] CHANG H C,WONG R X.Textural and biochemical properties of cobia (Rachycentron canadum) sashimi tenderised with the ultrasonic water bath[J].Food Chemistry,2012,132(3):1340-1345.
[42] 陈旭.日粮中添加发酵麸皮多糖和过瘤胃甜菜碱对杜寒杂交羊肉品质、抗氧化功能和风味物质组成的影响[D].呼和浩特:内蒙古农业大学,2022. CHEN X.Effects of fermented bran polysaccharide and rumen betaine on quality antioxidant and flavor composition of Duchan-Han hybrid lamb[D].Hohhot:Inner Mongolia Agricultural University,2022.(in Chinese)
[43] CHIKWANHA O C,MOELICH E,GOUWS P,et al.Effects of feeding increasing levels of grape (Vitis vinifera cv.Pinotage) pomace on lamb shelf-life and eating quality[J].Meat Science,2019,157:107887.
[44] 季寅辰.黄芪多糖和发酵麸皮多糖对肉羊肉品质、风味物质组成及货架期的影响[D].呼和浩特:内蒙古农业大学,2023. JI Y C.Effects of Astragalus polysaccharide and fermented bran polysaccharide and on meat and mutton quality,flavor composition and shelf life[D].Hohhot:Inner Mongolia Agricultural University,2023.(in Chinese)