产蛋高峰期与后期山麻鸭卵泡发育、抗氧化能力和自噬水平的比较

doi:10.16431/j.cnki.1671-7236.2024.11.013

中国畜牧兽医 ›› 2024, Vol. 51 ›› Issue (11): 4783-4791.doi: 10.16431/j.cnki.1671-7236.2024.11.013

• 营养与饲料 • 上一篇

产蛋高峰期与后期山麻鸭卵泡发育、抗氧化能力和自噬水平的比较

姜力莹¹, 马曼婷², 李凯潮², 张亚男², 赖跃^1,2, 陈伟², 王爽², 夏伟光², 金成龙², 郭亮¹, 郑春田²

1. 天津农学院动物科学与动物医学学院, 天津市农业动物繁育与健康养殖重点实验室, 天津 300392;
2. 广东省农业科学院动物科学研究所, 猪禽种业全国重点实验室, 农业农村部华南动物营养与饲料重点实验室, 广东省动物育种与营养公共实验室, 广东省畜禽育种与营养研究重点实验室, 广州 510640

收稿日期:2024-05-13 发布日期:2024-10-31
通讯作者: 郭亮 E-mail:3214662004@qq.com
作者简介:姜力莹,E-mail:jly9349@163.com;马曼婷,E-mail:476175621@qq.com;郑春田,E-mail:zhengcht@163.com。
基金资助:
财政部和农业农村部-国家现代农业产业技术体系(CARS-42-13);广东省水禽产业技术创新团队(2023KJ137);广东省农业农村厅畜禽全产业链标准化示范基地创建项目;蛋鸭笼养与精准营养供给技术研究与示范推广;广东省农业科学院高水平科技创新战略建设专项资金(202106TD、R2021PY-QY005)

Comparison of Follicle Development,Antioxidant Capacity and Autophagy Level Between Shan-ma Duck at Peak and Late Stages of Laying

JIANG Liying¹, MA Manting², LI Kaichao², ZHANG Yanan², LAI Yue^1,2, CHEN Wei², WANG Shuang², XIA Weiguang², JIN Chenglong², GUO Liang¹, ZHENG Chuntian²

1. Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300392, China;
2. Institute of Animal Science, Guangdong Academy of Agricultural Sciences, State Key Laboratory of Swine and Poultry Breeding Industry, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, China

Received:2024-05-13 Published:2024-10-31

摘要/Abstract

摘要： 【目的】探讨不同产蛋阶段山麻鸭的卵泡发育、促性腺激素水平、抗氧化能力及卵泡自噬相关基因的变化规律，为开发延长蛋禽产蛋期新技术奠定研究基础。【方法】采用单因素完全随机试验设计，选取产蛋正常的产蛋高峰期(25周)和产蛋后期(70周)的龙岩山麻鸭各84只，随机各分为6个重复，每个重复14只。饲喂同一玉米-豆粕型基础饲粮，试验期8周。于第8周末，随机从每个重复选取3只体况良好、产蛋正常的蛋鸭采血、屠宰取样，检测各项血液生化指标及卵巢发育相关指标。【结果】与产蛋高峰期山麻鸭相比，①产蛋后期山麻鸭的小黄卵泡数量、小黄卵泡指数及平均小黄卵泡重均显著降低，闭锁卵泡重量显著增加(P＜0.05)。②产蛋后期山麻鸭血浆中卵泡刺激素(FSH)水平显著降低(P＜0.05)；小黄卵泡的谷胱甘肽过氧化物酶和总超氧化物歧化酶活性显著降低、丙二醛水平显著升高，此外，闭锁卵泡的谷胱甘肽过氧化物酶和总超氧化物歧化酶活性显著降低(P＜0.05)。③产蛋后期山麻鸭卵泡中促卵泡素受体(FSHR) mRNA表达水平降低，叉头转录因子1(FOXO1)被激活并调控自噬相关基因(选择性自噬受体(SQSTM1)和beclin 1(BECN1)下调)(P＜0.05)。【结论】山麻鸭进入产蛋后期，由于氧化应激增加，卵泡中FSH含量及敏感性降低，激活FOXO1调控卵泡颗粒细胞自噬，抑制卵泡发育。

关键词: 山麻鸭; 卵泡发育; 抗氧化能力; 自噬

Abstract: 【Objective】 To investigate the changes of follicle development,gonadotropin levels,antioxidant capacity and follicle autophagy related genes in ducks at different laying stages,and to lay a research foundation for developing new technology to prolong laying period of laying birds. 【Method】 The single factor completely randomized experimental design was adopted,and 84 normal laying ducks (Longyan Shan-ma duck) in the peak laying stage (25 weeks) and the late laying stage (70 weeks) were selected.These ducks were randomly divided into 6 replicates,with 14 ducks in each replicate.The ducks were fed with the same corn-soybean meal basal diet for 8 weeks.At the end of the 8th week,3 healthy and normal laying ducks were randomly selected from each replicate for blood collection and slaughter sampling to detect blood biochemical indicators and ovarian development indicators. 【Result】 Compared with the duck in peak laying stage,①The number of small yellow follicles,the index of small yellow follicles and the average weight of small yellow follicles of laying duck in late laying stage were significantly reduced,and the weight of atretic follicles was significantly increased (P＜0.05).②The plasma follicle-stimulating hormone (FSH) level of laying duck in late laying stage was significantly lower (P＜0.05),the activities of glutathione peroxidase (GSH-Px) and total superoxide dismutase (T-SOD) in small yellow follicles and the malondialdehyde (MDA) level were significantly higher,and the activities of GSH-Px and T-SOD in atretic follicles were significantly lower (P＜0.05).③The mRNA expression level of follicle-stimulating hormone receptor (FSHR) in follicles of laying duck in late laying stage was reduced,and forkhead transcription factor 1 (FOXO1) was activated and regulated autophagy-related genes (selective autophagy receptor (SQSTM1) and beclin 1 (BECN1)) (P＜0.05). 【Conclusion】 When Shan-ma ducks entered the late laying period,the content and sensitivity of FSH in follicles were decreased due to the increase of oxidative stress,and FOXO1 was activated to regulate the autophagy of follicular granulosa cells and inhibit follicular development.

Key words: Shan-ma duck; follicle development; antioxidant capacity; autophagy

中图分类号:

S834⁺.83

姜力莹, 马曼婷, 李凯潮, 张亚男, 赖跃, 陈伟, 王爽, 夏伟光, 金成龙, 郭亮, 郑春田. 产蛋高峰期与后期山麻鸭卵泡发育、抗氧化能力和自噬水平的比较[J]. 中国畜牧兽医, 2024, 51(11): 4783-4791.

JIANG Liying, MA Manting, LI Kaichao, ZHANG Yanan, LAI Yue, CHEN Wei, WANG Shuang, XIA Weiguang, JIN Chenglong, GUO Liang, ZHENG Chuntian. Comparison of Follicle Development,Antioxidant Capacity and Autophagy Level Between Shan-ma Duck at Peak and Late Stages of Laying[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(11): 4783-4791.

参考文献

[1] 林东文,李益明,陈小春,等.龙岩山麻鸭产业状况调查与发展建议[J].福建畜牧兽医，2016,38(1):26-28.
LIN D W,LI Y M,CHEN X C,et al.Investigation and development suggestion of Longyan Shanma duck industry[J].Fujian Journal of Animal Husbandry and Veterinary Medicine,2016,38(1)：26-28.(in Chinese)
[2] 陈开丰.不同笼养条件下山麻鸭生产性能和生理状态比较分析[D].南昌:江西农业大学,2019.
CHEN K F.Comparison of production performance and physiological status in Shan partridge duck under different cage conditions[D].Nanchang:Jiangxi Agricultural University,2019.(in Chinese)
[3] MILLER R A,HARRISON D E,ASTLE C M,et al.Rapamycin,but not resveratrol or simvastatin,extends life span of genetically heterogeneous mice[J]. Journals of Gerontology Series A Biological Sciences and Medical Sciences,2011，66:191-201.
[4] STOOP D,COBO A,SILBER S.Fertility preservation for age-related fertility decline[J].Lancet,2014,384(9950):1311-1319.
[5] HAO E Y,CHEN H,WANG D H,et al.Melatonin regulates the ovarian function and enhances follicle growth in aging laying hens via activating the mammalian target of rapamycin pathway[J].Poultry Science,2020,99(4):2185-2195.
[6] GRAY A,FELDMAN H A,MCKINLAY J B,et al.Age,disease,and changing sex hormone levels in middle-aged men:Results of the massachusetts male aging study[J].Journal of Clinical Endocrinology & Metabolism,1991,73(5):1016-1025.
[7] MORLEY J E,KAISER F E,PERRY H R,et al.Longitudinal changes in testosterone,luteinizing hormone,and follicle-stimulating hormone in healthy older men[J].Metabolism,1997,46(4):410-413.
[8] MULLIGAN T,IRANMANESH A,JOHNSON M L,et al.Aging alters feed-forward and feedback linkages between LH and testosterone in healthy men[J].American Journal of Physiology,1997,273(4):R1407-R1413.
[9] FERRINI R L,BARRETT-CONNOR E.Sex hormones and age:A cross-sectional study of testosterone and estradiol and their bioavailable fractions in community-dwelling men[J].American Journal of Epidemiology,1998,147(8):750-754.
[10] DU Y,LIU L,HE Y,et al.Endocrine and genetic factors affecting egg laying performance in chickens:A review[J].British Poultry Science,2020,61(5):538-549.
[11] CICCONE N A,SHARP P J,WILSON P W,et al.Changes in reproductive neuroendocrine mRNAs with decreasing ovarian function in ageing hens[J]. Gene and Comparative Endocrinology,2005,144(1):20-27.
[12] HAN S,WANG J,CUI C,et al.Fibromodulin is involved in autophagy and apoptosis of granulosa cells affecting the follicular atresia in chicken[J].Poultry Science,2022,101(1):101524.
[13] YADAV P K,TIWARI M,GUPTA A,et al.Germ cell depletion from mammalian ovary:Possible involvement of apoptosis and autophagy[J]. Journal of Biomedical Science,2018,25(1):36.
[14] MIZUSHIMA N.Autophagy:Process and function[J].Genes Development,2007,21(22):2861-2873.
[15] SHEN M,JIANG Y,GUAN Z,et al.Protective mechanism of FSH against oxidative damage in mouse ovarian granulosa cells by repressing autophagy[J].Autophagy,2017,13(8):1364-1385.
[16] YOSHIMURA Y,BARUA A.Female reproductive system and immunology[J].Advances in Experimental Medicine and Biology,2017,1001:33-57.
[17] ONAGBESAN O,BRUGGEMAN V,DECUYPERE E.Intra-ovarian growth factors regulating ovarian function in avian species:A review[J].Animal Reproduction Science,2009,111(2-4):121-140.
[18] ZHOU S,ZHAO A,WU Y,et al.Protective effect of grape seed proanthocyanidins on oxidative damage of chicken follicular granulosa cells by inhibiting FOXO1-mediated autophagy[J].Frontiers in Cell and Developmental Biology,2022,10:762228.
[19] LIM J,NAKAMURA B N,MOHAR I,et al.Glutamate cysteine ligase modifier subunit (Gclm) null mice have increased ovarian oxidative stress and accelerated age-related ovarian failure[J].Endocrinology,2015,156(9):3329-3343.
[20] FADDY M J,GOSDEN R G,GOUGEON A,et al.Accelerated disappearance of ovarian follicles in mid-life:Implications for forecasting menopause[J].Human Reproduction,1992,7(10):1342-1346.
[21] YAO J,MA Y,ZHOU S,et al.Metformin prevents follicular atresia in aging laying chickens through activation of PI3K/AKT and calcium signaling pathways[J].Oxidative Medicine and Cellular Longevity,2020,2020:3648040.
[22] COLELLA M,CUOMO D,PELUSO T,et al.Ovarian aging:Role of pituitary-ovarian axis hormones and ncrnas in regulating ovarian mitochondrial activity[J].Front Endocrinol (Lausanne),2021,12:791071.
[23] CHECURA C M,BEG M A,PARRISH J J,et al.Positive effect of FSH but not LH on early development of the dominant follicle in mares[J].Reproduction Fertility and Development,2010,22(7):1092-1099.
[24] BAER G,LOUMAYE E.Comparison of recombinant human luteinising hormone (r-hLH) and human menopausal gonadotropin (hMG) in assisted reproductive technology[J].Current Medical Research and Opinion,2003,19(2):83-88.
[25] WANG L,TANG J,WANG L,et al.Oxidative stress in oocyte aging and female reproduction[J].Journal of Cellular Physiology,2021,236(12):7966-7983.
[26] 刘兴廷.衰老蛋鸡卵巢氧化应激缓解措施及其机理的研究[D].杭州:浙江大学,2018.
LIU X T.attenuation of the oxidative stress in the ovaries of the aging laving chickens[D].Hangzhou,Zhejiang University,2018.(in Chinese)
[27] ZHOU J,YAO W,LI C,et al.Administration of follicle-stimulating hormone induces autophagy via upregulation of HIF-1alpha in mouse granulosa cells[J].Cell Death & Disease,2017,8(8):e3001.
[28] TANG X R,MA L Z,GUO S,et al.High doses of FSH induce autophagy in bovine ovarian granulosa cells via the AKT/mTOR pathway[J].Reproduction in Domestic Animal,2021,56(2):324-332.
[29] SHEN M,JIANG Y,GUAN Z,et al.Protective mechanism of FSH against oxidative damage in mouse ovarian granulosa cells by repressing autophagy[J].Autophagy,2017,13(8):1364-1385.
[30] JOHNSON P A,STEPHENS C S,GILES J R.The domestic chicken:Causes and consequences of an egg a day[J].Poultry Science,2015,94(4):816-820.
[31] 陈悦群.年龄与颗粒细胞卵泡刺激素受体表达的关系[D].杭州:浙江大学，2011.
CHEN Y Q.Age and expression of follicle-stimulating hormone receptor in granulosa cells[D].Hangzhou:Zhejiang University,2011.(in Chinese)
[32] LIU Z,RUDD M D,HERNANDEZ-GONZALEZ I,et al.FSH and FOXO1 regulate genes in the sterol/steroid and lipid biosynthetic pathways in granulosa cells[J].Journal of Molecular Endocrinology,2009,23(5):649-661.

产蛋高峰期与后期山麻鸭卵泡发育、抗氧化能力和自噬水平的比较

Comparison of Follicle Development,Antioxidant Capacity and Autophagy Level Between Shan-ma Duck at Peak and Late Stages of Laying

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