家禽骨骼肌肌纤维类型影响因素及其转化的研究进展

doi:10.16431/j.cnki.1671-7236.2024.04.024

摘要/Abstract

摘要： 肌纤维是构成肌肉组织的基本单位。肌肉的肌纤维类型组成与肉品质密切相关,并可在机体生长发育、营养和饲养环境等外界刺激下发生变化。近年来,由于消费者和育种学家对肉品质的关注,家禽肌纤维类型及其转换规律已成为本领域的研究热点之一。随着组学研究技术的发展,转录组学、蛋白组学等技术已广泛应用于家禽肌纤维类型调控机理的研究,极大地推动了相关研究的进展。充分了解目前有关肌纤维类型转化的研究,是后续开展相关肉品质与调控机制研究的重要理论基础。在此背景下,作者介绍了肌纤维的结构组成及不同类型的分类方法,简述了肌纤维类型与家禽肌肉品质特征相关性状如肉色、pH、系水力和嫩度之间的关系,并总结了生长、品种、环境和饲养方式等因素对肌纤维类型的影响,重点讨论了现有的分子调控研究进展,旨在为家禽肌纤维类型转换机理的研究提供参考。最后,对未来优质肉鸡的育种方向提出了展望,希望随着现代分子生物技术的发展,单细胞/空间组学技术、基于CRISPR/Cas9系统的基因编辑技术等前沿技术能在肌纤维类型研究领域广泛运用,肌纤维类型转换的机制研究将不断完善,并建立家禽肉质性状的分子选育方法。

关键词: 家禽; 肉品质; 肌纤维类型; 肌纤维类型转换

Abstract: Muscle fibers are the basic units that form into muscle tissue.The muscle fiber types are closely related to meat quality,and commonly altered by external stimuli such as body growth and development,nutrition,and feeding environment.In recent years,studies on the formation and conversion patterns of poultry muscle fiber types have become the hotpot due to the attention given by consumers and breeders to meat quality.In addition,with the development of omics research technology,transcriptomics,proteomics and other technologies have been widely applied in the regulatory mechanism study of poultry muscle fiber types,which has greatly promoted relevant research progress.To fully understand the current research on muscle fiber type transformation is an important theoretical basis for the subsequent research on related meat quality and regulatory mechanism.The author introduces the composition of muscle fiber and the classification methods of different types of poultry muscle fiber.Additionally,the associations between the muscle fiber type and poultry different meat qualities such as color,pH,water binding capacity and tenderness traits are briefly described.This paper also summarizes the effects of growth,breed,environment,and feeding procedures on muscle fiber type,and focuses on the existing advances in molecular regulation studies,aiming to provide basic theoretical support and reference for future research.Finally,the future breeding direction of high-quality broilers is proposed.It is hoped that with the development of modern molecular biotechnology,cutting-edge technologies such as single cell/space omics technology,gene editing technology based on CRISPR/ Cas9 system can be widely used in the field of muscle fiber type research,constantly improve the mechanism of muscle fiber type conversion and establish the molecular breeding method of meat quality traits in poultry.

Key words: poultry; meat quality; muscle fiber type; muscle fiber type conversion

中图分类号:

谢兵红, 单艳菊, 范晨宇, 薛夫光, 吴红翔, 巨晓军, 束婧婷, 刘一帆. 家禽骨骼肌肌纤维类型影响因素及其转化的研究进展[J]. 中国畜牧兽医, 2024, 51(4): 1561-1572.

XIE Binghong, SHAN Yanju, FAN Chenyu, XUE Fuguang, WU Hongxiang, JU Xiaojun, SHU Jingting, LIU Yifan. Research Progress on Influencing Factors and Transformation of Skeletal Muscle Fiber Types in Poultry[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(4): 1561-1572.

参考文献

[1] LISTRAT A,LEBRET B,LOUVEAU I,et al.How muscle structure and composition influence meat and flesh quality[J].Scientific World Journal,2016,2016:3182746.
[2] LEE S H,JOO S T,RYU Y C.Skeletal muscle fiber type and myofibrillar proteins in relation to meat quality[J].Meat Science,2010,86(1):166-170.
[3] HUXLEY A F,NIEDERGERKE R.Structural changes in muscle during contraction:Interference microscopy of living muscle fibres.[J].Nature,1954,173(4412):971-973.
[4] SCHIAFFINO S.Fibre types in skeletal muscle:A personal account[J].Acta Physiologica,2010,199(4):451-463.
[5] HÄMÄLÄINEN N,PETTE D.Patterns of myosin isoforms in mammalian skeletal muscle fibres[J].Microscopy Research and Technique,1995,30(5):381-389.
[6] ENGEL W K.The essentiality of histo- and cytochemical studies of skeletal muscle in the investigation of neuromuscular disease[J].Neurology,1998,51(3):778-794.
[7] BROOKE M H,KAISER K K.Muscle fiber types:How many and what kind?[J].JAMA Neurology,1970,23(4):369-379.
[8] BROOKE M H,KAISER K K.Three "myosin adenosine triphosphatase" systems:The nature of their pH lability and sulfhydryl dependence[J].Journal Histochemistry & Cytochemistry,1970,18(9):670-672.
[9] HÅKAN W,JOSEPH D B,ABRAM K.Skeletal muscle:Energy metabolism,fiber types,fatigue and adaptability[J].Experimental Cell Research,2010,316(18):3007-3108.
[10] SCHIAFFINO S,REGGIANI C.Fiber types in mammalian skeletal muscles[J].Physiological Reviews,2011,91(4):1447-1531.
[11] BOTTINELLI R,REGGIANI C.Human skeletal muscle fibres:Molecular and functional diversity[J].Progress in Biophysics and Molecular Biology,2000,73(2-4):195-262.
[12] ISMAIL I,JOO S T.Poultry meat quality in relation to muscle growth and muscle fiber characteristics[J].Korean Journal for Food Science of Animal Resources,2017,37(6):873-883.
[13] PICARD B,GAGAOUA M.Muscle fiber properties in cattle and their relationships with meat qualities:An overview.[J].Journal of Agricultural and Food Chemistry,2020,68(22):6021-6039.
[14] OGILVIE R W,FEEBACK D L.A metachromatic dye-ATPase method for the simultaneous identification of skeletal muscle fiber types Ⅰ,ⅡA,ⅡB and ⅡC[J].Stain Technology,1990,65(5):231-241.
[15] 朱道立.跖肌肌球蛋白腺苷三磷酸酶与琥珀酸脱氢酶染色的相关性探讨[J].动物学杂志,2003,2:32-35. ZHU D L.A correlative approach in the investigation of myosin adenosine triphosphatase and succinate dehydrogenase in plantais muscles[J].Chinese Journal of Zoology,2003,2:32-35.(in Chinese)
[16] WENG K,HUO W,LI Y,et al.Fiber characteristics and meat quality of different muscular tissues from slow- and fast-growing broilers[J].Poultry Science,2022,101(1):101537.
[17] LUO L,MA W,LIANG K,et al.Spatial metabolomics reveals skeletal myofiber subtypes[J].Science Advances,2023,9(5):0455.
[18] JU X,LIU Y,SHAN Y,et al.Analysis of potential regulatory lncRNAs and circRNAs in the oxidative myofiber and glycolytic myofiber of chickens[J].Scientific Reports,2021,11(1):20861.
[19] WILKINS L J,BROWN S N,PHILLIPS A J,et al.Variation in the colour of broiler breast fillets in the UK[J].British Poultry Science,2000,41(3):308-312.
[20] CAMERON F,QUN S,RICHARD M,et al.Myoglobin and lipid oxidation interactions:Mechanistic bases and control[J].Meat Science,2010,86(1):86-94.
[21] BERRI C,LE BIHAN-DUVAL E,DEBUT M,et al.Consequence of muscle hypertrophy on characteristics of pectoralis major muscle and breast meat quality of broiler chickens[J].Journal of Animal Science,2007,85(8):2005-2011.
[22] JORDAN W,MARIANE B,JUAN F M G,et al.Muscle energy metabolism,growth,and meat quality in beef cattle[J].Agriculture,2019,9(9):195.
[23] CHOI Y M,HWANG S,LEE K.Comparison of muscle fiber and meat quality characteristics in different Japanese quail lines[J].Asian-Australasian Journal Animal Sciences,2016,29(9):1331-1337.
[24] ANNETTE S,KATJA R,PETER P P,et al.Physiological and structural events post mortem of importance for drip loss in pork[J].Meat Science,2002,61(4):355-366.
[25] CONG J,ZHANG L,LI J,et al.Effects of dietary supplementation with carnosine on growth performance,meat quality,antioxidant capacity and muscle fiber characteristics in broiler chickens[J].Journal of the Science of Food and Agriculture,2017,97(11):3733-3741.
[26] 陈宽维,李慧芳,张学余,等.肉鸡肌纤维与肉质关系研究[J].中国畜牧杂志,2002,38(6):6-7. CHEN K W,LI H F,ZHANG X Y,et al.Study on the relation between muscle fiber and meat quality in broilers[J].Chinese Journal of Animal Science,2002,38(6):6-7.(in Chinese)
[27] MEHMOOD W,ZHANG C.Correlations between muscle fibers characteristics and meat quality attributes of biceps femoris muscle:A comparative study of 2 distinctive broiler breeds[J].Brazilian Journal of Poultry Science,2020,22(4):001-008.
[28] 王春青,李侠,张春晖,等.肌原纤维特性与鸡肉原料肉品质的关系[J].中国农业科学,2014,47(10):2003-2012. WANG Q C,LI X,ZHANG C H,et al.Study on relationship between myofibril characteristics and meat quality of chicken raw meat[J].Scientia Agricultura Sinica,2014,47(10):2003-2012.(in Chinese)
[29] HU H,WANG J,ZHU R,et al.Effect of myosin heavy chain composition of muscles on meat quality in Laiwu pigs and Duroc[J].Science in China Series C:Life Sciences,2008,51(2):127-132.
[30] 苗海明,高爱武,杨金丽,等.动物肌内脂肪沉积对肉嫩度影响的研究进展[J].中国农学通报,2012,28(11):51-54. MIAO H M,GAO A W,YANG J L,et al.The advances of the effects of intramuscular fat depositionon the meat tenderness[J].Chinese Agricultural Science Bulletin,2012,28(11):51-54.(in Chinese)
[31] STOCKDALE F E,MILLER J B.The cellular basis of myosin heavy chain isoform expression during development of avian skeletal muscles[J].Developmental Biology,1987,123(1):1-9.
[32] CHRIST B,ORDAHL C P.Early stages of chick somite development[J].Anatomy and Embryology,1995,191(5):381-396.
[33] BUCKINGHAM M,BAJARD L,CHANG T,et al.The formation of skeletal muscle:From somite to limb[J].Journal of Anatomy,2003,202(1):59-68.
[34] GRIFONE R,LACLEF C,SPITZ F,et al.Six1 and Eya1 expression can reprogram adult muscle from the slow-twitch phenotype into the fast-twitch phenotype[J].Molecular and Cellular Biology,2004,24(14):6253-6267.
[35] SMITH J H.Relation of body size to muscle cell size and number in the chicken[J].Poultry Science,1963,42(2):283-290.
[36] FLVCK M,HOPPELER H.Molecular basis of skeletal muscle plasticity—From gene to form and function[J].Reviews of Physiology,Biochemistry and Pharmacology,2003,146:159-216.
[37] WENG K,HUO W,GU T,et al.Effects of marketable ages on meat quality through fiber characteristics in the goose[J].Poultry Science,2021,100(2):728-737.
[38] 葛雅琼,秦昊,高强,等.鸡骨骼肌纤维类型鉴定及CAPNCAPN1基因与鸡腿肌纤维类型比例的关联分析[J].中国家禽,2016,38(11):9-13. GE Y Q,QIN H,GAO Q,et al.Polymorphisms of CAPN1 gene and the association with myofiber types in skeletal muscle of chicken[J].China Poultry,2016,38(11):9-13.(in Chinese)
[39] VERDIGLIONE R,CASSANDRO M.Characterization of muscle fiber type in the pectoralis major muscle of slow-growing local and commercial chicken strains[J].Poultry Science,2013,92(9):2433-2437.
[40] HUO W,WENG K,GU T,et al.Effect of muscle fiber characteristics on meat quality in fast- and slow-growing ducks[J].Poultry Science,2021,100(8):101264.
[41] 李玥,袁丽霞,杨晓静,等.早期和后期限饲对肉鸡腓肠肌肌纤维类型和生长轴相关基因表达的影响[J].动物学报,2006,6:1133-1141. LI Y,YUAN L X,YANG X J,et al.Effects of early and late feed restriction on myofiber types and expression of growth-related genes in gastrocnemius of broiler chickens[J].Current Zoology,2006,6:1133-1141.(in Chinese)
[42] 徐苏微,邢通,李蛟龙,等.牛磺酸对肉鸡肉品质、糖酵解潜力及肌纤维特性的影响[J].南京农业大学学报,2020,43(5):935-941. XU S W,XING T,LI J L,et al.Effects of dietary taurine supplementation on meat quality,glycolytic potential and muscle fiber characteristics in broiler chickens[J].Journal of Nanjing Agricultural University,2020,43(5):935-941.(in Chinese)
[43] XUE J,FANG C,MU R,et al.Potential mechanism and effects of different selenium sources and different effective microorganism supplementation levels on growth performance,meat quality,and muscle fiber characteristics of Three-Yellow chickens[J].Frontiers in Nutrition,2022,9:869540.
[44] DANKOWIAKOWSK A,BOGUCK A,SOBOLEWSK A,et al.Effects of in ovo injection of prebiotics and synbiotics on the productive performance and microstructural features of the superficial pectoral muscle in broiler chickens[J].Poultry Science,2019,98(10):5157-5165.
[45] 张封东.胚蛋给养N-氨甲酰谷氨酸对肉仔鸡肉品质的影响[D].北京:中国农业科学院,2020. ZHANG F D.Effects of in ovo feeding of N-carbamylglutamate on meat quality of broilers[D].Beijing:Chinese Academy of Agricultural Sciences,2020.(in Chinese)
[46] 王松波,邓琳,赵婕,等.热应激对肉鸡抗氧化能力及腓肠肌纤维类型的影响[J].华南农业大学学报,2015,36(6):23-28. WANG S B,DENG L,ZHAO J,et al.Effects of heat stress on antioxidant capacity and gastrocnemius musclefiber types of broilers[J].Journal of South China Agricultural University,2015,36(6):23-28.(in Chinese)
[47] 陈若楠,胡波,杨朝永,等.不同饲养方式对科宝鸡肌纤维发育、肉品质及屠宰性能的影响[J].中国家禽,2022,44(7):77-83. CHEN R N,HU B,YANG C Y,et al.Effects of different rearing methods on muscle fiber development,meat quality and slaughter performance of Cobb broiler chicken[J].China Poultry,2022,44(7):77-83.(in Chinese)
[48] BRANCIARI R,MUGNAI C,MAMMOLI R,et al.Effect of genotype and rearing system on chicken behavior and muscle fiber characteristics[J].Journal of Animal Science,2009,87(12):4109-4117.
[49] SUN Y,LIU C,LI Y,et al.Effect of cage and floor housing systems on muscle fiber characteristics,carcass characteristics,and meat quality of slow-growing meat-type chickens[J].Agriculture,2023,13(2):365.
[50] 周娟,孟祥琼,秦鸿楠,等.不同海拔牦牛骨骼肌肌纤维类型和MYHC基因表达的比较[J].中国兽医学报,2022,42(6):1263-1269. ZHOU J,MENG Y Q,QIN H N,et al.Comparative on skeletal muscle fiber types and MYHC gene expression in yaks at different altitudes[J].Chinese Jourmal of Veterinary Science,2022,42(6):1263-1269.(in Chinese)
[51] 刘凡,殷志康,刘辉,等.藏猪与大白猪食用品质与肌纤维类型的相关性研究[J].食品工业科技,2021,42(24):271-277. LIU F,YIN Z K,LIU H,et al.Correlation between meat quality and muscle fiber types of Tibetan and Large White pigs[J].Science and Technology of Food Industry,2021,42(24):271-277.(in Chinese)
[52] 刘建,刘杰,丁九阳,等.低氧高糖条件下家兔骨骼肌Ⅰ型纤维向Ⅱ型纤维转化的作用[J].局解手术学杂志,2019,28(2):99-103. LIU J,LIU J,DING J Y,et al.Effect of low oxygen and high glucose on skeletal muscle Ⅰ type fiber transformed into Ⅱ fiber in rabbit[J].Journal of Regional Anatomy and Operative Surgery,2019,28(2):99-103.(in Chinese)
[53] BANNOW L I,BONATERRA G A,BERTOUNE M,et al.Effect of chronic intermittent hypoxia (CIH) on neuromuscular junctions and mitochondria in slow- and fast-twitch skeletal muscles of mice—The role of iNOS[J].Skeletal Muscle,2022,12(1):6-23.
[54] 李富贵,刘嘉,穆晓鹏,等.低海拔饲养条件下藏鸡屠宰性能及肉品质分析[J].四川农业大学学报,2017,35(4):562-567. LI F G,LIU J,MU X P,et al.Study on slaughter performance and meat quality of Tibetan chicken raised in low-altitude environment[J].Journal of Sichuan Agricultural University,2017,35(4):562-567.(in Chinese)
[55] MAYNE C N,SUTHERLAND H,JARVIS J C,et al.Induction of a fast-oxidative phenotype by chronic muscle stimulation:Histochemical and metabolic studies[J].The American Journal of Physiology,1996,270(1):313-320.
[56] MURGIA M,SERRANO A L,CALABRIA E,et al.Ras is involved in nerve-activity-dependent regulation of muscle genes[J].Nature Cell Biology,2000,2(3):142-147.
[57] WU H,ROTHERMEL B,KANATOUS S,et al.Activation of MEF2 by muscle activity is mediated through a calcineurin-dependent pathway[J].The EMBO Journal,2001,20(22):6414-6423.
[58] SERRANO A L,MURGIA M,PALLAFACCHINA G,et al.Calcineurin controls nerve activity-dependent specification of slow skeletal muscle fibers but not muscle growth[J].Proceedings of the National Academy of Sciences of the United States of America,2001,98(23):13108-13113.
[59] LIN J,WU H,TARR P T,et al.Transcriptional co-activator PGC-1 alpha drives the formation of slow-twitch muscle fibres[J].Nature,2002,418(6899):797-801.
[60] 朱文奇,徐文娟,束婧婷,等.鸭骨骼肌早期发育过程中钙蛋白酶3(CAPN3)基因的表达及其与肌纤维性状的关联[J].畜牧兽医学报,2014,45(3):385-390. ZHU W Q,XU W J,SHU J T,et al.Expression of CAPN3 gene in skeletal muscles and its association with myofiber traits during embryonic and early post-hatching development in ducks[J]. Acta Veterinaria et Zootechnica Sinica,2014,45(3):385-390.(in Chinese)
[61] 束婧婷,宋迟,徐文娟,等.IGF-Ⅰ-CaN-NFATc3信号通路相关基因在鸭发育早期骨骼肌中的同步表达及其与肌纤维性状相关性[J].中国农业科学,2015,48(6):1195-1204. SHU J T,SONG C,XU W J,et al.Expression profile of IGF-Ⅰ-calcineurin-NFATc3-dependent pathway genes in skeletal muscles and their associations with myofiber traits during embryonic and early post-hatching development in ducks[J].Scientia Agricultura Sinica,2015,48(6):1195-1204.(in Chinese)
[62] 盛中伟,单艳菊,束婧婷,等.CaN/NFAT信号通路相关基因在鸡骨骼肌中的表达规律及关联分析[J].畜牧兽医学报,2017,48(10):1825-1832. SHENG Z W,SHAN Y J,SHU J T,et al.The expression profile of CaN/NFAT-dependent pathway genes and their correlation analysis in chicken skeletal muscle[J].Acta Veterinaria et Zootechnica Sinica,2017,48(10):1825-1832.(in Chinese)
[63] 姬改革,单艳菊,章明,等.鸭发育早期骨骼肌TNNI1基因mRNA表达及与肌纤维生长的相关性分析[J].安徽农业大学学报,2017,44(6):992-995. JI G G,SHAN Y J,ZHANG M,et al.Expression profiles of the TNNI1 gene in the skeletal muscle and its correlation with the muscle fiber growth in early developmental ducks[J]. Journal of Anhui Agricultural University,2017,44(6):992-995.(in Chinese)
[64] 章明,单艳菊,姬改革,等.PRKAG3基因在鸡不同部位肌肉中的表达及其与肌纤维类型的相关性[J].江苏农业科学,2021,49(16):144-147. ZHANG M,SHAN Y J,JI G G,et al.Expression of PRKAG3 gene in different parts of chicken muscle and its correlation with muscle fiber types[J]. Jiangsu Agricultural Sciences,2021,49(16):144-147.(in Chinese)
[65] SHAN Y,JI G,ZOU J,et al.PGC-1α differentially regulates the mRNA expression profiles of genes related to myofiber type specificity in chicken[J].Journal of Integrative Agriculture,2020,19(8):2083-2094.
[66] UEDA M,WATANABE K,SATO K,et al.Possible role for avPGC-1alpha in the control of expression of fiber type,along with avUCP and avANT mRNAs in the skeletal muscles of cold-exposed chickens[J].FEBS Letters,2005,579(1):11-17.
[67] 束婧婷,单艳菊,姬改革,等.广西麻鸡m⁶A甲基转移酶基因表达与肌纤维类型及成肌分化的关系[J].中国农业科学,2022,55(3):589-601. SHU J T,SHAN Y J,JI G G,et al.Relationship between expression levels of Guangxi partridge chicken m⁶A methyltransferase genes,myofiber types and myogenic differentiation[J].Scientia Agricultura Sinica,2022,55(3):589-601.(in Chinese)
[68] 庞立川,单艳菊,刘一帆,等.METTL16在鸡不同类型肌肉中的表达规律及其对肌肉功能的调控作用[J].畜牧兽医学报,2023,54(2):545-553. PANG L C,SHAN Y J,LIU Y F,et al.Expression of METTL16 in different types of chicken muscle and its regulatory role in chicken skeletal muscle function[J]. Acta Veterinaria et Zootechnica Sinica,2023,54(2):545-553.(in Chinese)
[69] MA M,CAI B,JIANG L,et al.lncRNA-Six1 is a target of miR-1611 that functions as a ceRNA to regulate Six1 protein expression and fiber type switching in chicken myogenesis[J].Cells,2018,7(12):243.
[70] BOLIN C,MANTING M,ZHEN Z,et al.circPTPN4 regulates myogenesis via the miR-499-3p/NAMPT axis[J].Journal of Animal Science and Biotechnology,2022,13(3):682-696.
[71] 束婧婷,姬改革,单艳菊,等.基于表达谱芯片挖掘鸡骨骼肌不同类型肌纤维的差异表达基因[J].中国农业科学,2017,50(14):2826-2836. SHU J T,JI G G,SHAN Y J,et al.Analysis of differential expression genes between different myofiber types in chicken skeletal muscle based on gene expression microarray[J].Scientia Agricultura Sinica,2017,50(14):2826-2836.(in Chinese)
[72] LIU Y F,ZHANG M,SHAN Y J,et al.Transcriptome sequencing analysis of the role of miR-499-5p and SOX6 in chicken skeletal myofiber specification[J].Frontiers in Genetics,2022,13:1008649.
[73] SHU J,XIAO Q,SHAN Y,et al.Oxidative and glycolytic skeletal muscles show marked differences in gene expression profile in Chinese Qingyuan partridge chickens[J].PLoS One,2017,12(8):e0183118.
[74] WANG H,SHEN Z,ZHOU X,et al.Identification of differentially expressed genes in different types of broiler skeletal muscle fibers using the RNA-Seq technique[J].BioMed Research International,2020,2020:9478949.
[75] ZHANG R,LIU H,LIU J,et al.Transcriptional profiling identifies location-specific and breed-specific differentially expressed genes in embryonic myogenesis in Anas platyrhynchos[J].PLoS One,2015,10(12):e0143378.
[76] LIU Y,ZHANG M,SHAN Y,et al.miRNA-mRNA network regulation in the skeletal muscle fiber phenotype of chickens revealed by integrated analysis of miRNAome and transcriptome[J].Scientific Reports,2020,10(1):10619.
[77] CHENG H,SONG S,PARK T S,et al.Proteolysis and changes in meat quality of chicken pectoralis major and iliotibialis muscles in relation to muscle fiber type distribution[J].Poultry Science,2022,101(12):102185.
[78] LIU J,LIANG X,ZHOU D,et al.Coupling of mitochondrial function and skeletal muscle fiber type by a miR-499/Fnip1/AMPK circuit[J].EMBO Molecular Medicine,2016,8(10):1212-1228.
[79] SHAN Y,JI G,ZHANG M,et al.Use of transcriptome sequencing to explore the effect of CSRP3 on chicken myoblasts[J].Journal of Integrative Agriculture,2023,22(4):1159-1171.
[80] PASUT A,JONES A E,RUDNICKI M A.Isolation and culture of individual myofibers and their satellite cells from adult skeletal muscle[J].Journal of Visualized Experiments,2013(73):e50074.
[81] 候晔,胡岸,张巧霞,等.猪骨骼肌单根肌纤维的分离与鉴定方法[J].农业生物技术学报,2017,25(10):1714-1720. HOU Y,HU A,ZHANG Q X,et al.Isolation and identification of single skeletal muscle fibers in pigs[J]. Journal of Agricultural Biotechnology,2017,25(10):1714-1720.(in Chinese)
[82] CHEMELLO F,BEAN C,CANCELLARA P,et al.Microgenomic analysis in skeletal muscle:Expression signatures of individual fast and slow myofibers[J].PLoS One,2011,6(2):e16807.
[83] BLACKBURN D M,LAZURE F,CORCHADO A H,et al.High-resolution genome-wide expression analysis of single myofibers using SMART-Seq[J].Journal of Biological Chemistry,2019,294(52):20097-20108.
[84] KALLABIS S,ABRAHAM L,MULLER S,et al.High-throughput proteomics fiber typing (ProFiT) for comprehensive characterization of single skeletal muscle fibers[J].Skelet Muscle,2020,10(1):7.