虾青素对脂多糖诱导乌鳢肝脏抗氧化、炎症和糖皮质激素受体蛋白表达的影响

doi:10.16431/j.cnki.1671-7236.2023.11.044

摘要/Abstract

摘要： 【目的】研究虾青素（AST）对脂多糖（LPS）诱导乌鳢肝脏抗氧化和炎症相关基因及糖皮质激素受体蛋白表达的影响。【方法】选取健康的乌鳢500尾（体重23.40 g±0.53 g），随机分为5组，每组5个重复，每个重复20尾，对照组投喂基础饲粮，试验组投喂分别添加0（LPS组）、50、100 和200 mg/kg虾青素的试验饲粮，试验期56 d。养殖期结束次日，试验组腹腔注射4 mg/kg BW的LPS，对照组注射等体积的PBS，24 h后取肝脏样品用以检测抗氧化及炎症基因表达量和糖皮质激素受体（GR）蛋白表达量。【结果】①与对照组相比，LPS组乌鳢肝脏白介素-6（IL-6）和核因子-κB p65（NF-κB p65）基因表达显著上调（P<0.05）；与LPS组相比，50、100和200 mg/kg虾青素组肝脏IL-6和NF-κB p65基因表达水平显著下调（P<0.05），IL-10和核因子-κB抑制蛋白（IκBα）基因表达显著上调（P<0.05）。②与对照组相比，LPS组乌鳢肝脏超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）、过氧化氢酶（CAT）和谷胱甘肽巯基转移酶（GST）基因相对表达量显著降低（P<0.05）；与LPS组相比，50、100和200 mg/kg虾青素组SOD、CAT、GST和GPx基因表达水平升高，除50 mg/kg虾青素组SOD基因相对表达量差异不显著外，其余差异均显著（P<0.05）。③LPS组乌鳢肝脏磷酸化NF-κB p65（p-NF-κB p65）蛋白表达量显著高于对照组（P<0.05）；与LPS处理组相比，50、100和200 mg/kg虾青素组p-NF-κB p65蛋白表达量显著下调（P<0.05），同时磷酸化糖皮质激素受体（p-GR）蛋白表达量显著上调（P<0.05）。【结论】50~200 mg/kg虾青素可通过GR-NF-κB途径缓解LPS诱导的乌鳢肝脏炎症和氧化损伤。

关键词: 虾青素; 乌鳢; 糖皮质激素受体; 炎症; 抗氧化

Abstract: 【Objective】 This experiment was conducted to investigate the effects of astaxanthin (AST) on the expression of antioxidation and inflammation related genes and glucocorticoid receptor (GR) proteins in liver of Channa argus induced by lipopolysaccharide (LPS).【Method】 500 healthy Channa argus (23.40 g±0.53 g) were selected and randomly divided into 5 groups, with 5 replicates in each group, and 20 in each replicate.The control group was fed with basic feed, and the test groups were fed with test feed containing 0 (LPS group), 50, 100 and 200 mg/kg astaxanthin, respectively.The test period was 56 days.At the end of the test period, the test group was intraperitoneally injected with 4 mg/kg BW LPS the next day, and the control group was injected with the same volume of PBS.After 24 h, liver samples were collected to detect the expression of antioxidant and inflammatory genes and glucocorticoid receptor(GR) protein.【Result】 ① Compared with the control group, the levels of interleukin-6 (IL-6) and nuclear factor-κB p65(NF-κB p65) genes in the liver of Channa argus were significantly upregulated in LPS group (P<0.05).Compared with LPS group, the liver IL-6 and NF-κB p65 genes expression levels were significantly downregulated in the 50, 100 and 200 mg/kg astaxanthin groups (P<0.05), while IL-10 and IκBα genes expression were significantly upregulated (P<0.05).② Compared with the control group, the relative expression levels of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and glutathione sulfotransferase (GST) genes in the liver of Channa argus in the LPS group were significantly lower (P<0.05).Compared with the LPS group, the expression levels of SOD, CAT, GST and GPx genes in the 50, 100 and 200 mg/kg astaxanthin groups increased, except for the non significant difference in relative expression levels of SOD gene in the 50 mg/kg astaxanthin group, all other differences were significant (P<0.05).③The expression level of phosphorylated NF-κB p65(p-NF-κB p65) protein in the liver of Channa argus in LPS group was significantly higher than that of control group (P<0.05).Compared with LPS group, the expression of p-NF-κB p65 protein in 50, 100 and 200 mg/kg astaxanthin group was significantly decreased (P<0.05), and the expression of phosphorylated glucocorticoid receptor (p-GR) protein was significantly increased (P<0.05).【Conclusion】 During the process of LPS induced liver inflammation and oxidative damage in Channa argus, astaxanthin exerts anti-inflammatory and antioxidant effects through the GR-NF-κB pathway at a dose of 50-200 mg/kg.

Key words: astaxanthin; Channa argus; glucocorticoid receptor; inflammation; antioxidant

中图分类号:

S963

赵磊, 王毅, 刘云卓, 许万鑫, 王桂芹, 李沐阳. 虾青素对脂多糖诱导乌鳢肝脏抗氧化、炎症和糖皮质激素受体蛋白表达的影响[J]. 中国畜牧兽医, 2023, 50(11): 4776-4782.

ZHAO Lei, WANG Yi, LIU Yunzhuo, XU Wanxin, WANG Guiqin, LI Muyang. Effects of Astaxanthin on the Expression of Antioxidation, Inflammation and Glucocorticoid Receptor Protein in Liver of Channa argus Induced by Lipopolysaccharide[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(11): 4776-4782.

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