饲粮中添加地衣芽孢杆菌和解淀粉芽孢杆菌对断奶仔猪生长性能及血液指标的影响

doi:10.16431/j.cnki.1671-7236.2022.07.012

摘要/Abstract

摘要： 【目的】研究饲粮中添加地衣芽孢杆菌和解淀粉芽孢杆菌对断奶仔猪生长性能、血常规、血清生化及抗炎抗氧化指标的影响。【方法】选取108头28日龄杜长大断奶仔猪，按体重分为3组，每组6个重复，每个重复6头。对照组饲喂基础饲粮，其余两组分别在基础饲粮中添加1 g/kg地衣芽孢杆菌、1 g/kg解淀粉芽孢杆菌，试验期28 d。在试验第14和28天，空腹称重并计算采食量，每个重复选取2头仔猪，前腔静脉采血并用血细胞分析仪和试剂盒方法测定血常规及血清生化指标。【结果】各组断奶仔猪之间的平均日增重、平均日采食量、料重比均无显著性差异（P＞0.05）。与对照组相比，解淀粉芽孢杆菌组断奶仔猪的腹泻率显著降低（P＜0.05）；地衣芽孢杆菌组和解淀粉芽孢杆菌组断奶仔猪第14天血液淋巴细胞百分含量均显著升高，白细胞介素-2（IL-2）、丙二醛含量及乳酸脱氢酶、谷草转氨酶活性均显著降低（P＜0.05）；解淀粉芽孢杆菌组断奶仔猪第14天血液中碱性磷酸酶活性显著降低，血红蛋白含量显著升高，第28天血液中IL-2含量显著降低，总蛋白、球蛋白含量均显著升高（P＜0.05）。【结论】饲粮中添加地衣芽孢杆菌或解淀粉芽孢杆菌对断奶仔猪生长性能无显著影响，但能够改善仔猪血液指标，保护肝脏功能，且添加解淀粉芽孢杆菌还可以提高仔猪新陈代谢，降低仔猪腹泻率。

关键词: 地衣芽孢杆菌; 解淀粉芽孢杆菌; 断奶仔猪; 生长性能; 血液指标

Abstract: 【Objective】 This study was conducted to investigate the effects of Bacillus licheniformis and Bacillus amyloliquefaciens supplementation on the growth performance,blood routine,serum biochemical indicators and antioxidant and inflammation indexes of weaned piglets.【Method】 A total of 108 28-day-old weaned piglets (Duroc×Landrace×Yorkshire) were selected and divided into 3 groups according to body weight,with 6 replicates per group and 6 piglets per replicate.The piglets in control group were fed with basal diet,and the other two groups were supplemented with 1 g/kg Bacillus licheniformis or 1 g/kg Bacillus amyloliquefaciens in the basal diet.The experiment lasted for 28 days.At the 14th and 28th days,fasting piglets were weighed and piglets intake were calculated,two piglets were selected from each replication,and the plasma samples were collected from the anterior vena cava,the blood parameters were detected by hematology analyzer and kit.【Result】 There were no significant difference in average daily gain (ADG),average daily feed intake (ADFI) and feed/gain ratio (F/G) among three experiment groups (P>0.05).Compared with control group,the diarrhea rate of weaned piglets in Bacillus amyloliquefaciens group was significantly decreased (P＜0.05).At the 14th day,the percentage of blood lymphocytes percentage of weaned piglets in Bacillus licheniformis and Bacillus amyloliquefaciens groups were significantly increased,while the contents of interleukin-2 (IL-2) and malondialdehyde (MDA),and the activities of lactate dehydrogenase and aspartate aminotransferase were significantly decreased (P＜0.05).At the 14th day,the serum alkaline phosphatase activity of weaned piglets in Bacillus amyloliquefaciens group was significantly decreased,while the hemoglobin was significantly increased (P＜0.05).At the 28th day,the content of IL-2 of weaned piglets in Bacillus amyloliquefaciens group was significantly decreased,while the contents of total protein and globulin were significantly increased (P＜0.05).【Conclusion】 The supplement of Bacillus licheniformis or Bacillus amyloliquefaciens had no outstanding effects on the growth performance,but it could improve the blood biochemical indicators and protect the liver of weaned piglets,and the supplement of Bacillus amyloliquefaciens could also improve the metabolism of piglets and decrease the diarrhea rate of piglets.

Key words: Bacillus licheniformis; Bacillus amyloliquefaciens; weaned piglets; growth performance; blood indicators

中图分类号:

S816.7

陈雪颖, 王慧晟, 吴杰, 邓波, 刁新平. 饲粮中添加地衣芽孢杆菌和解淀粉芽孢杆菌对断奶仔猪生长性能及血液指标的影响[J]. 中国畜牧兽医, 2022, 49(7): 2547-2556.

CHEN Xueying, WANG Huisheng, WU Jie, DENG Bo, DIAO Xinping. Effects of Dietary Bacillus licheniformis and Bacillus amyloliquefaciens on Growth Performance and Blood Indicators of Weaned Piglets[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2547-2556.

参考文献

[1] GASKINS H R,COLLIER C T,ANDERSON D B.Antibiotics as growth promotants:Mode of action[J].Animal Biotechnology,2002,13(1):29-42.
[2] SHEN D S,TAO X Q,SHENTU J L,et al.Residues of veterinary antibiotics in pig feeds and manures in Zhejiang province[J].Advanced Materials Research,2014,1010-1012:301-304.
[3] 肖怡,陶大勇,赵明明,等.地衣芽孢杆菌对肉羊甲烷排放及消化代谢的影响[J].动物营养学报,2016,28(2):515-523. XIAO Y,TAO D Y,ZHAO M M,et al.Effects of Baclicus lincheniformis on methane emission,digestion and metabolism of mutton sheep[J].Chinese Journal of Animal Nutrition,2016,28(2):515-523.(in Chinese)
[4] 栾素军,邢焕,孙永波,等.解淀粉芽孢杆菌在畜禽养殖中的应用研究进展[J].中国畜牧兽医,2016,43(10):2615-2620. LUAN S J,XING H,SUN Y B,et al.Research progress on Bacillus amyloliquefaciens in animal breeding industry[J].China Animal Husbandry & Veterinary Medicine,2016,43(10):2615-2620.(in Chinese)
[5] WANG Y,DU W,LEI K,et al.Effects of dietary Bacillus licheniformis on gut physical barrier,immunity,and reproductive hormones of laying hens[J].Probiotics and Antimicrobial Proteins,2017,9(3):292-299.
[6] LIU X L,YAN H,LV L,et al.Growth performance and meat quality of broiler chickens supplemented with Bacillus licheniformis in drinking water[J].Asian-Australasian Journal of Animal Sciences,2012,25(5):682-689.
[7] 戴利铭,李岚岚,刘一贤,等.解淀粉芽孢杆菌生防菌BS-3全基因组测序及生物信息分析[J].微生物学通报,2021,48(6):2073-2088. DAI L M,LI L L,LIU Y X,et al.Whole genome sequencing and genomics analysis of Bacillus amyloliquefaciens BS-3 with biocontrol activity[J].Microbiology China,2021,48(6):2073-2088.(in Chinese)
[8] ALFONZO A,PICCOLO S L,CONIGLIARO G,et al.Antifungal peptides produced by Bacillus amyloliquefaciens AG1 active against grapevine fungal pathogens[J].Annals of Microbiology,2012,62(4):1593-1599.
[9] 曹广添.解淀粉芽孢杆菌的抑菌机理及对肉鸡生长代谢和盲肠微生物菌群的影响[D].杭州:浙江大学,2015. CAO G T.Effects of Bacillus amyloliquefaciens on the growth,metabolites and diversity of cecalmicroflora in broilers and its antibacterial mechanism[D].Hangzhou:Zhejiang University,2015.(in Chinese)
[10] JI J,HU S L,ZHENG M Z,et al.Bacillus amyloliquefaciens SC06 inhibits ETEC-induced pro-inflammatory responses by suppression of MAPK signaling pathways in IPEC-1 cells and diarrhea in weaned piglets[J].Livestock Science,2013,158(1-3):206-214.
[11] ZONG X,WANG T H,LU Z Q,et al.Effects of Clostridium butyricum or in combination with Bacillus licheniformis on the growth performance,blood indexes,and intestinal barrier function of weanling piglets[J].Livestock Science,2019,220:137-142.
[12] 邓文,徐彬,孙全友,等.地衣芽孢杆菌和低聚木糖对断奶仔猪生长性能、肠道形态和通透性及粪便微生物的影响[J].中国饲料,2019,15:40-44. DENG W,XU B,SUN Q Y,et al.Effects of Bacillus licheniformis and xylooligosaccharides on the performance,intestinal morphology and permeability,and fecal microbes of weaned piglets[J].China Feed,2019,15:40-44.(in Chinese)
[13] WIZNA H,ABBAS Y,RIZAL A,et al.The effect of supplementation of micro nutrient on nutrient rice bran which fermented by Bacillus amyloliquefaciens[J].Pakistan Journal of Nutrition,2012,11(5):439-443.
[14] WANG H,YANG L,PING Y G,et al.Engineering of a Bacillus amyloliquefaciens strain with high neutral protease producing capacity and optimization of its fermentation conditions[J].PLoS One,2016,11(1):e0146373.
[15] LEI X J,RU Y J,ZHANG H F.Effect of Bacillus amyloliquefaciens-based direct-fed microbials and antibiotic on performance,nutrient digestibility,cecalmicroflora,and intestinal morphology in broiler chickens[J].Journal of Applied Poultry Research,2014,23(3):486-493.
[16] 尹会方,姚志翔,杨守深,等.丁酸梭菌和地衣芽孢杆菌对断奶仔猪生长性能及血液指标的影响[J].中国饲料,2019,20:33-36. YIN H F,YAO Z X,YANG S S,et al.Effects of Clostridium butyricum,Bacillus licheniformis on the growth performance and blood indexes of piglets[J].China Feed,2019,20:33-36.(in Chinese)
[17] 徐小明,白建勇,宦海琳,等.地衣芽孢杆菌对发酵床饲养仔猪生长性能、消化酶活性及肠道主要菌群数量的影响[J].中国畜牧兽医,2015,42(4):923-928. XU X M,BAI J Y,HUAN H L,et al.Effect of Bacillus licheniformis on growth performance,digestive enzymes and the number of intestinal main microbial flora of piglets raised in fermentation bed[J].China Animal Husbandry & Veterinary Medicine,2015,42(4):923-928.(in Chinese)
[18] 廖柱,蔡旭旺,谢红涛,等.添加植物乳杆菌与枯草芽孢杆菌对仔猪生长性能和血清生化指标的影响[J].饲料研究,2021,44(21):32-35. LIAO Z,CAI X W,XIE H T,et al.Effect of Lactobacillus plantarum and Bacillus subtilis supplementation on growth performance and serum biochemical indices of piglets[J].Feed Research,2021,44(21):32-35.(in Chinese)
[19] PEET-SCHWERING C,VERHEIJEN R,JRGENSEN L,et al.Effects of a mixture of Bacillus amyloliquefaciens and Bacillus subtilis on the performance of growing-finishing pigs[J].Animal Feed Science and Technology,2020,261:114409.
[20] STROMPFOVA V,MARCINAKOVA M,SIMONOVA M,et al.Enterococcus faecium EK13--An enterocin A-Producing strain with probiotic character and its effect in piglets[J].Anaerobe,2006,12(5/6):242-248.
[21] 张闻桐,郭亮,李杰,等.益生菌与宿主疾病关系的研究进展[J].微生物学杂志,2020,40(4):90-97. ZHAGN W T,GUO L,LI J,et al.Advances in relationship between probiotics and host disease[J].Journal of Microbiology,2020,40(4):90-97.(in Chinese)
[22] 王淑梅,邸维,妥彦峰,等.益生菌的免疫调控作用研究进展[J].粮食与油脂,2021,34(5):23-26. WANG S M,DI W,TUO Y F,et al.Research progress on immune regulation of probiotics[J].Cereals & Oils,2021,34(5):23-26.(in Chinese)
[23] 冯宝宝,方伟,封飞飞,等.屎肠球菌SF68对断奶仔猪生长性能、血常规和血清生化指标及抗氧化性能的影响[J].动物营养学报,2018,30(3):1125-1135. FENG B B,FANG W,FENG F F,et al.Effects of Enterococcus faecalis SF68 on growth performance,blood routine and serum biochemical indexes and antioxidant ability of weaned piglets[J].Chinese Journal of Animal Nutrition,2018,30(3):1125-1135.(in Chinese)
[24] 郭军蕊,董晓芳,佟建明.枯草芽孢杆菌CGMCC 1.921对蛋鸡生产性能、血常规指标、血清生化指标及免疫球蛋白含量的影响[J].动物营养学报,2017,29(2):465-478. GUO J R,DONG X F,TONG J M.Effects of Bacillus subtilis CGMCC 1.921 on performance,routine blood parameters,serum biochemical parameters and immunoglobulin contents of laying hens[J].Chinese Journal of Animal Nutrition,2017,29(2):465-478.(in Chinese)
[25] 张安荣,刘娇,陈志敏,等.饲粮添加枯草芽孢杆菌对肉仔鸡生长性能、血清生化指标和胫骨指标的影响[J].动物营养学报,2020,32(9):4123-4131. ZHANG A R,LIU J,CHEN Z M,et al.Effect of dietary Bacillus subtilis on growth performance,serum biochemical indices and tibia index of broilers[J].Chinese Journal of Animal Nutrition,2020,32(9):4123-4131.(in Chinese)
[26] 卢佳伟,王金刚,高琴,等.不同益生菌对断奶山羊生长性能、血清生化指标以及粪便菌群的影响[J].动物营养学报,2021,33(5):2752-2764. LU J W,WANG J G,GAO Q,et al.Effects of different probiotics on growth performance,serum biochemical indexes and fecal microflora of weaned goats[J].Chinese Journal of Animal Nutrition,2021,33(5):2752-2764.(in Chinese)
[27] 袁丰华,林黑着,李卓佳,等.地衣芽孢杆菌对尖吻鲈血液生理生化指标的影响[J].南方水产,2009,5(2):45-50. YUAN F H,LIN H Z,LI Z J,et al.Effects of dietary Bacillus licheniformis on blood physiological-biochemical indices in cultured Latescalarifer[J].South China Fisheries Science,2009,5(2):45-50.(in Chinese)
[28] JONES J G.Hepatic glucose and lipid metabolism[J].Diabetologia,2016,59(6):1098-1103.
[29] 闫蕾,赵彩彦.肝脏--一个新的免疫器官[J].国际内科学杂志,2009,36(4):206-210. YAN L,ZHAO C Y.Liver--A new immune organ[J].International Journal of Internal Medicine,2009,36(4):206-210.(in Chinese)
[30] 唐武,蔡巧利,蒋慧姣,等.饲粮添加益生菌和合生元对环江香猪生长性能及血浆生化参数的影响[J].动物营养学报,2021,33(1):546-552. TANG W,CAI Q L,JIANG H J,et al.Effects of detary supplementation with probiotics and synbiotics on growth performance and plasma biochemical parameters of Huanjiang Mini-piglets[J].Chinese Journal of Animal Nutrition,2021,33(1):546-552.(in Chinese)
[31] LI X T,LV Z M,CHEN J,et al.Bacillus amyloliquefaciens B10 can alleviate liver apoptosis and oxidative stress induced by aflatoxin B₁[J].Food and Chemical Toxicology,2021,151:112-124.
[32] 张立永,任海洋.地衣芽孢杆菌对雏鸡生长性能、肠道结构及血清生化指标的影响[J].黑龙江畜牧兽医,2019,12:113-116. ZHANG L Y,REN H Y.Effects of Bacillus licheniformis on growth performance,intestinal structure and serum biochemical indexes of chicks[J].Heilongjiang Animal Science and Veterinary Medicine,2019,12:113-116.(in Chinese)
[33] 尹清强,李小飞,常娟,等.微生态制剂对哺乳和断奶仔猪生产性能的影响及作用机理研究[J].动物营养学报,2011,23(4):622-630. YIN Q Q,LI X F,CHANG J,et al.Probiotics:Effects on performance and mechanisms of action in suckling and weaner piglets[J].Chinese Journal of Animal Nutrition,2011,23(4):622-630.(in Chinese)
[34] WANG Y,WU Y P,WANG B K,et al.Effects of probiotic Bacillus as a substitute for antibiotics on antioxidant capacity and intestinal autophagy of piglets[J].AMB Express,2017,7(1):52.
[35] 应志雄,李悦,张昊,等.解淀粉芽孢杆菌对免疫抑制肉鸡生长性能和肝脏抗氧化功能的影响[J].中国兽医学报,2018,38(8):1609-1615. YING Z X,LI Y,ZHANG H,et al.Effects of Bacillus amyloliquefaciens on growth performance and liver antioxidant function of immunosuppressed broilers[J].Chinese Journal of Veterinary Medicine,2018,38(8):1609-1615.(in Chinese)
[36] 杨家军,钱坤,章薇,等.地衣芽孢杆菌对肉鸡生产性能、抗氧化及营养物质代谢的影响研究[J].中国兽医学报,2014,34(5):736-739. YANG J J,QIAN K,ZHANG W,et al.Effect of supplementary Bacillus licheniformis on growth performance,antioxidation and nutrient metabolism in Partridge shank chicken[J].Chinese Journal of Veterinary Medicine,2014,34(5):736-739.(in Chinese)
[37] 贺驭,孔祥峰,王振勇,等.益生菌抗氧化机制的研究进展[J].中国家禽,2017,39(24):41-44. HE Y,KONG X F,WANG Z Y,et al.Advances in antioxidant mechanisms of probiotics[J].China Poultry,2017,39(24):41-44.(in Chinese)
[38] 刘煜珺,张雨晴,高原,等.乳杆菌胞外多糖抗氧化活性研究[J].中国食品学报,2019,19(6):21-35. LIU Y J,ZHAGN Y Q,GAO Y,et al.Studies on the antioxidative activity of exopolysaccharide from Lactobacillus strains[J].Journal of Chinese Institute of Food Science and Technology,2019,19(6):21-35.(in Chinese)
[39] 熊传帅,黎力之,周建旭,等.精氨酸对动物肠道炎症的影响及其在畜牧生产上的应用研究进展[J].中国畜牧杂志,2021,57(12):20-25. XIONG C S,LI L Z,ZHOU J X,et al.Advances in effects of arginine on intestinal inflammation in animals and its application in animal husbandry[J].Chinese Journal of Animal Science,2021,57(12):20-25.(in Chinese)