饮水中添加复合益生菌对岭南黄羽肉鸡生长性能和肠道微生物菌群结构的影响

doi:10.16431/j.cnki.1671-7236.2022.01.011

摘要/Abstract

摘要： [目的] 研究饮水中添加复合益生菌对岭南黄羽肉鸡生长性能和肠道微生物菌群结构的影响。[方法] 选取270只21日龄健康快大型岭南黄羽肉鸡，随机分为3组，对照组饲喂基础饲粮，抗生素组饲喂基础饲粮+300 g/t 15%金霉素和40 g/t 50%维吉尼亚霉素，益生菌组饲喂基础饲粮+复合益生菌（饮水），其中，复合益生菌制剂为1：1：1配伍的罗伊氏乳杆菌、植物乳杆菌和酿酒酵母，各菌种添加量为1.0×10⁶ CFU/mL，试验期5周，全程自由采食和饮水。[结果] ①22~42日龄，与对照组相比，益生菌组和抗生素组的料重比（F/G）均显著降低（P<0.05）；22~56日龄，与对照组相比，益生菌组和抗生素组的平均日增重（ADG）均显著提高（P<0.05），且益生菌组死淘率最低，均一性优于对照组与抗生素组。②42日龄时，抗生素组回肠Observed Species指数、Shannon指数和Ace指数均显著高于对照组和益生菌组（P<0.05），盲肠Observed Species指数显著低于对照组和益生菌组（P<0.05）；③益生菌组在厚壁菌门、乳杆菌属的相对丰度上占有优势，而其他各菌门、各菌属中抗生素组都呈现出较高的相对丰度，益生菌组与对照组表现出相同的趋势。[结论] 饮水中添加复合益生菌可以显著改善22~56日龄岭南黄羽肉鸡的生长性能，有效降低死淘率，整体效果明显优于对照组，接近抗生素组，有部分高效替抗功能；在肠道微生物方面，益生菌组整体上表现出与对照组更大的相似性，说明饮水中添加复合益生菌对肠道微生物的影响不大。

关键词: 复合益生菌; 黄羽肉鸡; 生长性能; 微生物组学

Abstract: [Objective] To study the effects of compound probiotics added to drinking water on growth performance and intestinal microflora in Lingnan Yellow feather broilers. [Method] 270 healthy and fast-sized Lingnan Yellow feather broilers at 21 days old were selected and randomly divided them into 3 groups. The control group was fed basal diet, the antibiotic group was fed basal diet+300 g/t 15% chlortetracycline and 40 g/t 50% virginiamycin, and the probiotics group fed with basic diet+drinking water supplemented with compound probiotics. Among them, the compound probiotic preparation was 1:1:1 compatible Lactobacillus reuteri, Lactobacillus plantarum and Saccharomyces cerevisiae, and the addition amount of each strain was 1.0×10⁶ CFU/mL. The test period was 5 weeks, and the whole process was free to eat and drink. [Results] ①At 22-42 days of age, compared with control group, the feed-to-weight ratio (F/G) of probiotic and antibiotic groups were significantly reduced (P<0.05);At 22-56 days of age, compared with control group, the average daily gain (ADG) of probiotic and antibiotic groups were significantly increased (P<0.05), and the probiotic group had the lowest mortality, and the uniformity was better than that of control and antibiotic groups. ②At 42 days of age, the ileal Observed Species index, Shannon index and Ace index of antibiotic group were significantly higher than that of control and probiotic groups (P<0.05), and the cecal Observed Species index of antibiotic group was significantly lower than that of control and probiotics groups (P<0.05). ③In terms of relative abundance of species, the probiotic group had an advantage in the relative abundance of Firmicutes and Lactobacillus, while the antibiotic group in the other phylums and genus all showed higher relative abundance, the probiotic and control groups showed the same trend. [Conclusion] Adding compound probiotics to drinking water could significantly improve the growth performance of Lingnan Yellow feather broilers at 22-56 days of age, and effectively reduce mortality, the overall effect was significantly better than that of control group, closed to the antibiotic group, and had part of the high-efficiency replacement function. In terms of the intestinal microbiome, the probiotic group showed greater similarity with control group as a whole, indicated that the addition of compound probiotics in drinking water had little effect on the intestinal microbes.

Key words: compound probiotics; Yellow feather broilers; growth performance; microbiomics

中图分类号:

S816.7

刘石, 刘静, 陈庄, 俞婷, 贝锦龙, 王蕾, 李拯民, 王志林. 饮水中添加复合益生菌对岭南黄羽肉鸡生长性能和肠道微生物菌群结构的影响[J]. 中国畜牧兽医, 2022, 49(1): 98-108.

LIU Shi, LIU Jing, CHEN Zhuang, YU Ting, BEI Jinlong, WANG Lei, LI Zhengmin, WANG Zhilin. Effects of Compound Probiotics Added to Drinking Water on Growth Performance and Intestinal Microflora in Lingnan Yellow Feather Broilers[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 98-108.

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