蒲鹿乳炎散水煎醇沉液抗炎镇痛作用研究

doi:10.16431/j.cnki.1671-7236.2021.05.028

摘要/Abstract

摘要： 试验旨在研究蒲鹿乳炎散水煎醇沉液的抗炎镇痛作用，为后期临床开发提供依据。采用二甲苯致小鼠耳廓肿胀、醋酸致小鼠腹腔毛细血管通透性增加、角叉菜胶致小鼠足趾肿胀法来研究其抗炎作用；采用福尔马林和热板仪致痛法来研究其镇痛作用。各试验均将小鼠随机分为5组，每组10只，雌雄各半，分别是阴性对照组、蒲鹿乳炎散低、中、高剂量组、阿司匹林阳性对照组（阿司匹林组）。蒲鹿乳炎散低、中、高剂量组及阿司匹林组分别每日以含生药20、30、40及0.15 mg/g的剂量灌胃给药1次，阴性对照组灌胃给予同等容积的生理盐水，均连续灌胃给药7 d，后续按照不同试验具体操作。小鼠耳廓肿胀试验结果显示，各用药组与阴性对照组相比，均可极显著降低二甲苯导致的小鼠耳廓肿胀（P<0.01）；与阿司匹林组相比，蒲鹿乳炎散中、高剂量组差异不显著（P>0.05）。醋酸致腹腔毛细血管通透性增高试验结果显示，与阴性对照组相比，各用药组对醋酸导致的通透性增高均具有极显著抑制作用（P<0.01）；与阿司匹林组相比，蒲鹿乳炎散低、中剂量组均差异极显著（P<0.01），而高剂量组差异不显著（P>0.05）。角叉菜胶所致足趾肿胀试验结果显示，各用药组与阴性对照组相比，均具有不同程度的肿胀抑制作用（P<0.01）；与阿司匹林组相比，蒲鹿乳炎散低剂量组差异极显著（P<0.01），但中、高剂量组差异均不显著（P>0.05）。福尔马林致痛试验结果显示，在Ⅰ相时间段内，各用药组与阴性对照组相比，均极显著减少小鼠舔足次数（P<0.01）；在Ⅱ相时间段内，各组两两之间差异均显著或极显著（P<0.05；P<0.01）。热板仪致痛试验结果显示，给药后各个时间，各用药组与阴性对照组相比，均能显著延长痛阈值（P<0.01）；与阿司匹林组相比，蒲鹿乳炎散高剂量组减小疼痛阈值的效果极显著强于阿司匹林组（P<0.01），低、中剂量组的效果极显著弱于阿司匹林组（P<0.01）。本试验结果表明，蒲鹿乳炎散水煎醇沉液对小鼠具有良好的抗炎镇痛作用。

关键词: 蒲鹿乳炎散; 水煎醇沉液; 抗炎; 镇痛

Abstract: The purpose of this experiment was to study the anti-inflammatory and analgesic effects of Pulu Ruyan San,and to provide the basis for the later clinical development.The anti-inflammatory effect was studied by xylene induced ear swelling,acetic acid induced peritoneal capillary permeability and carrageenan induced toe swelling in mice.The analgesic effect was studied by formalin and hot plate apparatus.In each experiment,mice were randomly divided into 5 groups, 10 mice in each group, half male and half female, which were negative control group, low, medium and high dose groups of Pulu Ruyan powder, and aspirin positive control group (aspirin group). The low, medium and high-dose groups of Pulu Ruyan powder and aspirin group were given 20, 30, 40 and 0.15 mg/g crude drugs by gavage once a day, respectively. The negative control group was given the same volume of normal saline by gavage for 7 days, and the follow-up was carried out according to different experiments.The results of mouse auricle swelling test showed that compared with the negative control group,each medication group could extremely significantly reduce the auricle swelling induced by xylene (P<0.01),but compared with aspirin group,there was no significant difference between Pulu Ruyan San middle and high-dose groups (P>0.05).The results of acetic acid induced peritoneal capillary permeability test showed that compared with the negative control group,each medication group had extremely significant inhibitory effect on acetic acid induced permeability increase (P<0.01).Compared with aspirin group,the difference between Pulu Ruyan San low and middle dose groups was very extremely significant (P<0.01),while the difference between high dose group was not significant (P>0.05).The results of carrageenan induced toe swelling test showed that compared with the negative control group,each medication group had different degrees of swelling inhibition (P<0.01).Compared with aspirin group,the difference was extremely significant in Pulu Ruyan San low dose group (P<0.01),but the difference was not significant in the middle and high-dose groups (P>0.05).The results of formalin induced pain test showed that compared with the negative control group,the times of licking feet in each treatment group were extremely significantly reduced in phase Ⅰ (P<0.01).In phase Ⅱ,the differences between each group were significant or extremely significant (P<0.05;P<0.01).The results of hot plate instrument pain test showed that compared with the negative control group,the pain threshold of each medication group was significantly prolonged at each time after administration (P<0.01).Compared with aspirin group,the effect of reducing pain threshold of Pulu Ruyan San high dose group was extremely significantly stronger than that of aspirin group (P<0.01),and the effects of Pulu Ruyan San low and middle dose groups were extremely significantly weaker than that of aspirin group (P<0.01).The results showed that Pulu Ruyan San had good anti-inflammatory and analgesic effects on mice.

Key words: Pulu Ruyan San; decoction and alcohol precipitation; anti-inflammatory; analgesia

中图分类号:

S853.7

蒋威, 杨洁, 宋朋杰, 柯群华, 马晓宇, 王胜义, 丁学智, 王东升, 严作廷. 蒲鹿乳炎散水煎醇沉液抗炎镇痛作用研究[J]. 中国畜牧兽医, 2021, 48(5): 1762-1771.

JIANG Wei, YANG Jie, SONG Pengjie, KE Qunhua, MA Xiaoyu, WANG Shengyi, DING Xuezhi, WANG Dongsheng, YAN Zuoting. Study on the Anti-inflammatory and Analgesic Effects of Pulu Ruyan San with Decoction and Alcohol Precipitation[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(5): 1762-1771.

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