复方忍冬藤提取物促进骨折愈合及抗炎作用研究

doi:10.16431/j.cnki.1671-7236.2017.01.039

摘要/Abstract

摘要：

试验旨在探讨复方忍冬藤提取物对骨折愈合和抗炎的作用。采用牙科电钻制备兔右侧桡骨骨折模型，通过测定血清中钙、磷和碱性磷酸酶的含量，探明其对骨折家兔血清生化指标的影响。利用LPS诱导的RAW264.7细胞建立炎症模型，采用ELISA法测定复方忍冬藤提取物对LPS诱导的RAW264.7细胞释放的炎症因子IL-6、TNF-α、IL-1β和NO含量的变化。结果显示，利用牙科电钻可成功制备兔骨折模型，复方忍冬藤提取物可增加骨折家兔血清中钙和碱性磷酸酶的水平，降低血清中磷含量。与LPS组相比，50~200 μg/mL复方忍冬藤提取物对LPS诱导的RAW264.7细胞分泌的炎症因子TNF-α、IL-1β和NO的含量具有显著的抑制作用（P<0.05），对IL-6的含量无显著影响（P>0.05）。综上所述，复方忍冬藤提取物对骨折愈合有促进作用，并具有较好的抗炎效果。

关键词: 复方忍冬藤; 提取物; 骨折愈合; 抗炎

Abstract:

This experiment was aimed to study the effects of mixed Caulis Lonicerae japonicae extracts on fracture healing and inflammation. Rabbit right radius fracture model was induced by dental drill in order to study the medicine's effect on fracture healing. Moreover,the contents of Ca,P and alkaline phosphatase in serum were measured to explore the influences of mixed Caulis Lonicerae japonicae extracts on serum biochemical indexes of fractured rabbits.RAW264.7 cell was induced by LPS in order to research the effect of medicine on inflammation. The concentrations of TNF-α,IL-1β,IL-6 and NO were measured by ELISA. The results showed that the rabbit fracture model was successfully prepared by dental drill, and the extracts of Caulis Lonicerae japonicae could increase the levels of Ca and alkaline phosphatase and reduce the P content in the serum of fractured rabbits. The TNF-α,IL-1β and NO levels in RAW264.7 cell induced by LPS were significantly inhibited by the extracts with concentrations ranging from 50 to 200 μg/mL (P<0.05). However, the extracts had no significant effect on the level of IL-6 (P>0.05). These results demonstrated that the extracts of mixed Caulis Lonicerae japonicae could promote the fracture healing and possessed good resistance inflammation effect.

Key words: mixed Caulis Lonicerae japonicae; extracts; fracture healing; anti-inflammation

中图分类号:

S858.291

贾海燕, 滕旭东, 王慧, 张慧珍. 复方忍冬藤提取物促进骨折愈合及抗炎作用研究[J]. 《中国畜牧兽医》, 2017, 44(1): 275-281.

JIA Hai-yan, TENG Xu-dong, WANG Hui, ZHANG Hui-zhen. Effects of Mixed Caulis Lonicerae japonicae Extracts on Fracture Healing and Anti-inflammation[J]. , 2017, 44(1): 275-281.

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