柑橘提取物对肥育猪后肠食糜蛋白质代谢产物的影响

doi:10.16431/j.cnki.1671-7236.2022.07.014

中国畜牧兽医 ›› 2022, Vol. 49 ›› Issue (7): 2567-2577.doi: 10.16431/j.cnki.1671-7236.2022.07.014

柑橘提取物对肥育猪后肠食糜蛋白质代谢产物的影响

崔艺燕¹, 鲁慧杰¹, 田志梅¹, 邓盾¹, 李贞明¹, 余苗¹, 马现永^1,2

1. 广东省农业科学院动物科学研究所, 畜禽育种国家重点实验室, 农业农村部华南动物营养与饲料重点实验室, 广东省畜禽育种与营养研究重点实验室, 广东畜禽肉品质量安全控制与评定工程技术研究中心, 广州 510640;
2. 岭南现代农业科学与技术广东省实验室茂名分中心, 茂名 525000

收稿日期:2021-12-20 出版日期:2022-07-05 发布日期:2022-06-29
通讯作者: 马现永 E-mail:407986619@qq.com
作者简介:崔艺燕,E-mail:958117076@qq.com。
基金资助:
省级农业科技创新推广及农业资源与生态环境保护建设项目（2021KJ266）；广东省农业科学院学科团队建设项目（202118TD）；饲料产业技术体系创新团队建设项目（2021KJ115）；科技创新战略专项资金（高水平农科院建设）——杰出人才（R2020PY-JC001）和优秀博士（R2020YJ-YB2002）；韶关市科技计划项目（200731114530834）；茂名实验室自主科研项目（2021ZZ003）

Effects of Citrus Extract on Protein Metabolites in the Hindgut Digesta of Finishing Pigs

CUI Yiyan¹, LU Huijie¹, TIAN Zhimei¹, DENG Dun¹, LI Zhenming¹, YU Miao¹, MA Xianyong^1,2

1. Guangdong Engineering Technology Research Center of Animal Meat Quality and Safety Control and Evaluation, Guangdong Key Laboratory of Animal Breeding and Nutrition, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, State Key Laboratory of Livestock and Poultry Breeding,Institute of Animal Science, Guangdong Academy of Agricultural Science, Guangzhou 510640, China;
2. Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Maoming 525000, China

Received:2021-12-20 Online:2022-07-05 Published:2022-06-29

摘要/Abstract

摘要： 【目的】通过研究柑橘提取物对肥育猪后肠食糜蛋白质代谢产物的影响，旨在为柑橘提取物从源头调控臭气的产生提供依据。【方法】选取108头56日龄（19.73±0.39）kg杜×长×大猪，公母各半，随机分为3组，每组分6栏，每栏6头猪。对照组饲喂基础饲粮，抗生素组在基础饲粮中添加75 g/t金霉素，柑橘提取物组在基础饲粮中添加柑橘提取物（56~112日龄添加250 mL/t柑橘提取物，113~194日龄添加200 mL/t柑橘提取物）。194日龄时收集肥育猪盲肠和结肠食糜，测定食糜中游离氨基酸、酚和吲哚类物质、生物胺的含量。【结果】①在盲肠食糜中，与对照组相比，柑橘提取物组和抗生素组牛磺酸、天冬氨酸、丙氨酸、缬氨酸、亮氨酸、赖氨酸、组氨酸、精氨酸、吲哚和甲胺含量均显著降低（P＜0.05）。与对照组相比，柑橘提取物有降低苏氨酸、丝氨酸、谷氨酸、甘氨酸、瓜氨酸、异亮氨酸、脯氨酸和粪臭素含量的趋势（0.05≤P＜0.10），苯酚、腐胺、酪胺和精胺含量均显著降低（P＜0.05）。与抗生素组相比，柑橘提取物组腐胺和酪胺含量均显著减少（P＜0.05）。②在结肠食糜中，与对照组相比，柑橘提取物组和抗生素组天冬氨酸、丝氨酸、异亮氨酸、胱硫醚、亮氨酸、γ-氨基丁酸、总氨基酸、苯酚、对甲酚、粪臭素和甲胺含量均显著降低（P＜0.05），脯氨酸和吲哚含量均有降低的趋势（0.05≤P＜0.10）。柑橘提取物组丝氨酸、异亮氨酸、胱硫醚、亮氨酸、总氨基酸和对甲酚含量均显著低于抗生素组（P＜0.05）；与对照组相比，柑橘提取物组苏氨酸、谷氨酸、甘氨酸、鸟氨酸和酪胺含量均显著降低（P＜0.05），亚精胺含量有降低的趋势（0.05≤P＜0.10）；与对照组和抗生素组相比，柑橘提取物组苯丙氨酸、色氨酸和赖氨酸含量均显著降低（P＜0.05）。【结论】柑橘提取物降低了肥育猪后肠食糜蛋白质代谢产物的含量，可减少臭气的产生。

关键词: 柑橘提取物; 猪; 后肠; 生物胺; 游离氨基酸

Abstract: 【Objective】 The effects of citrus extract on protein metabolites in the hindgut digesta of finishing pigs were studied in this experiment,in order to provide basis for the regulation of citrus extract on the generation of odor from the source.【Method】 One hundred and eight 56-day-old Duroc×Landrace×Large White pigs weighted (19.73±0.39) kg were randomly assigned to 3 groups with 6 pans per group and 6 pigs per pan (same male and female).Pigs in the control group were fed basal diets,pigs in the antibiotic group were fed the basal diets supplemented with 75 g/t chlortetracycline,and pigs in the citrus extract group were fed the basal diets supplemented with citrus extract (250 mL/t during 56 to 112 days of age and 200 mL/t during 113 to 194 days of age).The cecal and colonic digesta was collected from finishing pigs at 194 days of age,and the contents of free amino acids,phenols,indoles,and biogenic amines in the digesta were determined.【Result】 ①In cecal digesta,compared to control group,adding citrus extract and antibiotic significantly decreased the levels of taurine,aspartic acid,alanine,valine,leucine,lysine,histidine,arginine,indole,and methylamine (P＜0.05).Adding citrus extract tended to decrease the levels of threonine,serine,glutamic acid,glycine,citrulline,isoleucine,proline,and skatole (0.05≤P＜0.10),significantly decreased the levels of phenol,putrescine,tyramine,and spermine (P＜0.05).Compared to antibiotic group,dietary with citrus extract decreased the levels of putrescine and tyramine (P＜0.05).②In colonic digesta,compared to control group,dietary with citrus extract and antibiotic significantly decreased the levels of aspartic acid,serine,isoleucine,cystathionine,leucine,γ-aminobutyric acid,total amino acids,phenol,p-cresol,skatole,and methylamine (P＜0.05),and tended to decrease the levels of proline and indole (0.05≤P＜0.10).The levels of serine,isoleucine,cystathionine,leucine,total amino acids, and p-cresol in the citrus extract group were significantly lower than those in antibiotic group (P＜0.05).Compared to control group,dietary with citrus extract decreased the levels of threonine,glutamic acid,glycine,ornithine,and tyramine (P＜0.05),and tended to decreased the level of spermidine (0.05≤P＜0.10).The levels of phenylalanine,tryptophan,and lysine in the citrus extract group were significantly lower than those in control and antibiotic groups (P＜0.05).【Conclusion】 Dietary with citrus extract decreased the protein metabolite levels in the hindgut digesta of finishing pigs,reduced the production of odor.

Key words: citrus extract; pig; hindgut; biogenic amine; free amino acid

中图分类号:

S816.7

崔艺燕, 鲁慧杰, 田志梅, 邓盾, 李贞明, 余苗, 马现永. 柑橘提取物对肥育猪后肠食糜蛋白质代谢产物的影响[J]. 中国畜牧兽医, 2022, 49(7): 2567-2577.

CUI Yiyan, LU Huijie, TIAN Zhimei, DENG Dun, LI Zhenming, YU Miao, MA Xianyong. Effects of Citrus Extract on Protein Metabolites in the Hindgut Digesta of Finishing Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2567-2577.

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柑橘提取物对肥育猪后肠食糜蛋白质代谢产物的影响

Effects of Citrus Extract on Protein Metabolites in the Hindgut Digesta of Finishing Pigs

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