螺旋藻抗炎和免疫增强作用的研究

doi:10.16431/j.cnki.1671-7236.2019.07.031

摘要/Abstract

摘要：

试验旨在探讨螺旋藻抗炎作用及其对机体免疫功能的影响。试验通过二甲苯致小鼠耳肿胀，构建小鼠体内炎症模型，以地塞米松为阳性对照药物，以小鼠耳肿胀为观察指标，探讨螺旋藻的体内抗炎作用；通过环磷酰胺构建小鼠免疫抑制模型，以不同剂量螺旋藻处理后测定免疫抑制小鼠及正常小鼠的脏器指数、血清白细胞介素2（IL-2）、白细胞介素6（IL-6）、白细胞介素1β（IL-1β）、肿瘤坏死因子α（TNF-α）、干扰素γ（IFN-γ）水平，同时结合脾脏及胸腺病理组织学观察，探讨螺旋藻对免疫抑制小鼠及正常小鼠免疫功能的影响。结果表明，在螺旋藻对小鼠体内抗炎作用影响的试验中，0.3%螺旋藻灌胃对小鼠耳肿胀的抑制率极显著高于地塞米松对照组和其他螺旋藻处理组（P<0.01），且螺旋藻对各试验组小鼠的脏器指数无不良影响，差异不显著（P>0.05）；在螺旋藻对小鼠免疫功能影响试验中，与空白对照组相比，环磷酰胺阳性对照组脾脏指数和胸腺指数极显著下降（P<0.01）；肝脏指数极显著上升（P<0.01），其它各剂量螺旋藻处理组小鼠胸腺指数跟空白对照组相比差异不显著（P>0.05），各组小鼠血清IL-2、IL-6、TNF-α、IFN-γ水平差异不显著（P>0.05）；通过病理组织学观察发现，环磷酰胺阳性对照组小鼠的脾小体萎缩、胸腺小体减少、淋巴细胞及网状细胞变性坏死，而各剂量螺旋藻处理组的脾小体和胸腺小体结构清晰完整、淋巴细胞增多。综上，螺旋藻能降低地塞米松和环磷酰胺对小鼠的免疫抑制，并且能修复小鼠脾脏和胸腺损伤，说明其在抗炎和缓解免疫抑制方面具有一定的作用。

关键词: 螺旋藻; 小鼠; 抗炎; 免疫功能

Abstract:

The aim of this experiment was to evaluate the anti-inflammatory effect and immune effect of Spirulina.An inflammation model of mice was constructed by xylene induced mice ear swelling,and the effect of Spirulina on anti-inflammatory was investigated by using dexamethasone in positive control group and mice ear swelling as observation indexes.An immune-suppression model of mice was set up with cyclophosphamide,and the immune effect of Spirulina was investigated by comparing the organ indexes,levels of IL-2,IL-6,IL-1β,TNF-α and IFN-γ,and spleen and thymus histopathology in both normal and immune-suppressed mice treated with different doses of Spirulina.The results showed that:In the anti-inflammatory experiment,the ear swelling inhibition rate of mice treated with 0.3% spirulina was extremely significantly higher than that of dexamethasone group and other Spirulina treatmens (P<0.01),and viscera index of all Spirulina groups had no significant difference (P>0.05);In the immune function experiment,compared with blank control group,the spleen index and thymus index were extremely significantly decreased (P<0.01),and the liver index was extremely significantly increased (P<0.01) in cyclophosphamide positive control group,and the thymus indexes of Spirulina treated groups had no significant difference (P>0.05).Serum IL-2,IL-6,TNF-α and IFN-γ levels had no significant difference among all the groups (P>0.05).At the same time,the decrease of thymus corpuscles and the degeneration and necrosis of lymphocytes and reticular cells were observed in the positive control group by histopathological observation,while the structure of spleen corpuscles and thymus corpuscles in Spirulina treatments were more clear and complete,and the lymphocytes were increased.In conclusion,Spirulina could reduce the immunosuppression of dexamethasone and cyclophosphamide on mice,and repair the damage of spleen and thymus in mice.Spirulina played a certain role in anti-inflammatory and alleviating immunosuppression.

Key words: Spirulina; mouse; anti-inflammatory; immune function

中图分类号:

R961

陈忠伟, 邱洁, 李晓玉, 赵武, 秦毅斌, 卢冰霞, 段群棚, 梁家幸, 李斌, 周英宁, 胡庭俊, 何颖. 螺旋藻抗炎和免疫增强作用的研究[J]. 《中国畜牧兽医》, 2019, 46(7): 2135-2143.

CHEN Zhongwei, QIU Jie, LI Xiaoyu, ZHAO Wu, QIN Yibin, LU Bingxia, DUAN Qunpeng, LIANG Jiaxing, LI Bin, ZHOU Yingning, HU Tingjun, HE Ying. Study on Anti-inflammatory and Immune Effect of Spirulina[J]. , 2019, 46(7): 2135-2143.

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