2016－2019年广西部分猪场仔猪病毒性腹泻的流行病学调查

doi:10.16431/j.cnki.1671-7236.2020.02.028

摘要/Abstract

摘要： 本研究旨在了解引起仔猪腹泻的主要病毒性病原感染情况及流行特点，为有效防控广西仔猪腹泻提供科学依据。试验采用RT-PCR/PCR检测方法对2016年3月至2019年2月广西14个地级市366个规模猪场送检的914份仔猪腹泻病料样品进行猪流行性腹泻病毒（PEDV）、猪轮状病毒（PoRV）、猪传染性胃肠炎病毒（TGEV）、猪Delta冠状病毒（PDCoV）、猪瘟病毒（CSFV）、猪繁殖与呼吸综合征病毒（PRRSV）、猪圆环病毒2型（PCV2）和猪伪狂犬病病毒（PRV）检测，并分析其阳性率在不同年份、季节、地区之间的差异及病原混合感染情况。调查结果显示，PEDV、PDCoV、PRRSV、PoRV、PCV2、CSFV、PRV均存在不同程度的感染，其样品平均阳性率分别为59.74%、8.32%、7.77%、4.92%、3.72%、3.28%和2.08%，未检测出TGEV；PEDV已无明显季节性，一年四季均高发，即使在炎热的夏季，在感染猪群中也持续存在，PDCoV有明显的季节性，多发生在冬春寒冷季节；广西仔猪腹泻PEDV阳性率高，单一感染高达50.55%，仔猪腹泻混合感染情况也较多，其中以二重感染情况较为常见，同时也存在四重感染现象。结果表明，广西腹泻仔猪存在7种病毒性病原不同程度感染情况，其中以PEDV导致的仔猪病毒性腹泻尤为严重，新发PDCoV阳性率仅次于PEDV，需重视并加强对新发PDCoV的防控。

关键词: 仔猪腹泻; 病毒性病原; 流行病学

Abstract: This study was aimed to understand main viral pathogens infection situation and popular characteristics of piglet diarrhea,and provide scientific basis for effective prevention and control of piglet diarrhea in Guangxi.In this experiment,RT-PCR and PCR methods were used to detect the pathogens of PEDV,PoRV,TGEV,PDCoV,CSFV,PRRSV,PCV2 and PRV of 914 diarrhea samples of piglets from 366 large-scale pig farms in 14 cities in Guangxi from 2016 to 2019,and analyzed these viruses pathogens mixed infection situation and the differences of the positive rates in different years,seasons and regions.According to the survey results,PEDV,PDCoV,PRRSV,PoRV,PCV2,CSFV and PRV all had different degrees of infection,and the average positive rate of the samples were 59.74%,8.32%,7.77%,4.92%,3.72%,3.28% and 2.08%,respectively,and no TGEV was detected.The PEDV had no significant seasonal variations,it was high all the year round.The PEDV still existed in infected piglet diarrhea,even in hot summer.However,the PDCoV had significant seasonal variations,it occurred frequently in both spring and winter.The positive rate of PEDV was high in Guangxi,the single infection rate was up to 50.55%.There were also many cases of diarrhea mixed infection in piglets,among which double infection was more common,and there were also four kinds of infection.The results showed that there were 7 kinds of viral pathogens infecting piglets in different degrees in Guangxi,among which the viral diarrhea caused by PEDV was particularly serious,and the positive rate of new PDCoV was second only to that of PEDV,so it was necessary to pay attention to and strengthen the prevention and control of new PDCoV.

Key words: piglet diarrhea; viral pathogen; epidemiology

中图分类号:

S858.28

段群棚, 李晓玉, 赵硕, 秦毅斌, 卢冰霞, 李斌, 苏乾莲, 赵武, 何颖, 梁家幸, 蒋冬福, 周英宁, 陈忠伟. 2016－2019年广西部分猪场仔猪病毒性腹泻的流行病学调查[J]. 中国畜牧兽医, 2020, 47(2): 564-574.

DUAN Qunpeng, LI Xiaoyu, ZHAO Shuo, QIN Yibin, LU Bingxia, LI Bin, SU Qianlian, ZHAO Wu, HE Ying, LIANG Jiaxing, JIANG Dongfu, ZHOU Yingning, CHEN Zhongwei. A Epidemiological Investigation on Diarrhea Viral Disease of Piglets in Some Pig Farms in Guangxi During 2016 to 2019[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(2): 564-574.

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