犬种布鲁氏菌研究进展

doi:10.16431/j.cnki.1671-7236.2021.10.033

摘要/Abstract

摘要： 犬种布鲁氏菌属于6个经典种布鲁氏菌之一，为天然粗糙型，毒力较弱。长期以来，由于犬种布鲁氏菌对人类致病性较低，其危害一直被忽视。自1966年在美国发现该菌以来，已传播至世界多地。近年来，随着养犬业的不断发展及宠物犬数量的增多，犬布鲁氏菌病发病率不断上升，在某些地区已成为流行性疫病，且对人类公共卫生安全的威胁也日益加重。目前，犬种布鲁氏菌感染导致的犬布鲁氏菌病尚无可靠的诊断方法及有效的疫苗。鉴于此，在查阅相关文献的基础上，笔者对犬种布鲁氏菌的病原学、流行病学、致病机理、检测方法、疫苗研究等方面的相关研究进展进行综述，以期为犬布鲁氏菌病的深入研究和防控提供参考。

关键词: 犬种布鲁氏菌; 流行病学; 致病机理; 检测方法; 疫苗

Abstract: Brucella canis is one of the six classical species of Brucella. It is a natural rough type with weak virulence. For a long time, the harm of Brucella canis has been ignored because of its low pathogenicity to human. Since it was found in the United States in 1966, it has spread to many places in the world. With the continuous development of the kennel industry and the increasing number of pet dogs, the incidence rate of canine brucellosis caused by Brucella canis has been increasing. In some areas, it has become an epidemic disease, and the threat to the public health security of human society is also increasing. There is no reliable diagnosis and effective vaccine for canine brucellosis caused by Brucella canis. In view of this, on the basis of consulting the relevant literature, this paper reviewed the etiology, epidemiology, pathogenic mechanism, test method, vaccine of Brucella canis, in order to provide meaningful reference for the prevention and control of canine brucellosis caused by Brucella canis.

Key words: Brucella canis; epidemiology; pathogenic mechanism; test method; vaccine

中图分类号:

S852.61⁺4

孙佳丽, 董浩, 彭小薇, 朱良全, 蒋卉, 冯宇, 李筱英, 丁家波, 王春凤. 犬种布鲁氏菌研究进展[J]. 中国畜牧兽医, 2021, 48(10): 3803-3811.

SUN Jiali, DONG Hao, PENG Xiaowei, ZHU Liangquan, JIANG Hui, FENG Yu, LI Xiaoying, DING Jiabo, WANG Chunfeng. Research Progress on Brucella canis[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3803-3811.

参考文献

[1] CARMICHAEL L.Abortions in 200 beagles[J]. Journal of the American Veterinary Medical Association, 1966, 149:1126.
[2] MEYER M E.Brucella organisms isolated from dogs:Comparison of characteristics of members of the genus Brucella[J]. American Journal of Veterinary Research, 1969, 30(10):1751.
[3] CORBELL M J, BRINLEY-MORGAN W J.Bergey's Manual of Systematic Bacteriology[M].USA:The Williams and Wilkings Co., 1984.
[4] HOYER B H, MCCULLOUGH N B.Homologies of deoxyribonucleic acids from Brucella ovis, canine abortion organisms, and other Brucella species[J]. Journal of Bacteriology, 1968, 96(5):1783-1790.
[5] VERGER J M, GRIMONT F, GRIMONT P A D, et al.Brucella, a monospecific genus as shown by deoxyribonucleic acid hybridization[J]. International Journal of Systematic Bacteriology, 1985, 35(3):292-295.
[6] FERREIRA VICENTE A, GUILLAUME G, YANNICK C, et al.New insights into phylogeography of worldwide Brucella canis isolates by comparative genomics-based approaches:Focus on Brazil[J]. BMC Genomics, 2018, 19(1):636.
[7] 姜吉喆, 潘航, 乐敏, 等.基于比较基因组学方法的世界范围的犬布鲁氏菌系统发育群研究[J]. 中国生物工程杂志, 2020, 40(3):38-47. JIANG J Z, PAN H, LE M, et al.The study of worldwide Brucella canis of phylogenetic groups by comparative genomics-based approaches[J]. China Biotechnology, 2020, 40(3):38-47.(in Chinese)
[8] 刘志国.犬种布鲁氏菌的遗传多样性分析[J]. 微生物学通报, 2019, 46(12):3325-3334. LIU Z G.Genetic diversity of Brucella canis[J]. Microbiology China, 2019, 46(12):3325-3334.(in Chinese)
[9] 程汝佳.犬种布鲁氏菌GB1株全基因组测序及其单克隆抗体制备和初步应用[D].泰安:山东农业大学, 2018. CHENG R J.Whole genome sequencing of Brucella canis GB1 and monoclonal antibody preparation and preliminary application[D].Tai'an:Shandong Agricultural University, 2018.(in Chinese)
[10] LARSON K L, SPICKLER A R, VIERA S, et al.Center for food security and public health[D].Ames:Iowa State University, 2012.
[11] HENSEL M E, NEGRON M, ARENAS-GAMBOA A M.Brucellosis in dogs and public health risk[J]. Emerging Infectious Diseases, 2018, 24(8):1401-1406.
[12] SANTOS R L, SOUZA T D, MOL J P S, et al.Canine brucellosis:An update[J]. Frontiers in Veterinary Science, 2021, 8:594291.
[13] GREENE C.Canine brucellosis[J]. Infectious Diseases of the Dog and Cat, 2006, 12:35-42.
[14] DALY R, WILLIS K C, WOOD J, et al.Seroprevalence of Brucella canis in dogs rescued from South Dakota Indian reservations, 2015-2019[J]. Preventive Veterinary Medicine, 2020, 184:105157.
[15] HUBBARD K, WANG M, SMITH D R.Seroprevalence of brucellosis in Mississippi shelter dogs[J]. Preventive Veterinary Medicine, 2018, 159:82-86.
[16] TVW A, GB B, DP C, et al.Seroprevalence of Brucella canis antibodies in dogs entering a Minnesota humane society, Minnesota, 2016-2017[J]. Preventive Veterinary Medicine, 2019, 168:90-94.
[17] GALARCE N, ESCOBAR B, MARTÍNEZ E, et al.Prevalence and genomic characterization of Brucella canis strains isolated from kennels, household, and stray dogs in Chile[J]. Animals, 2020, 10(11):2073.
[18] CADMUS S I B, ADESOKAN H K, AJALA O O, et al.Seroprevalence of Brucella abortus and B.canis in household dogs in Southwestern Nigeria:A preliminary report[J]. Journal of the South African Veterinary Association-tydskrif Van Die Suid-afrikaanse Veterinere Vereniging, 2012, 82(1):56-57.
[19] COMFORT A M, JOSEPH O A, OLUWATOYIN A V, et al.Sero-epidemiological survey and risk factors associated with brucellosis in dogs in South-western Nigeria[J]. The Pan African Medical Journal, 2016, 23:29.
[20] YOAK A J, REECE J F, GEHRT S D, et al.Disease control through fertility control:Secondary benefits of animal birth control in Indian street dogs[J]. Preventive Veterinary Medicine, 2014, 113(1):152-156.
[21] JAMIL T, MELZER F, KHAN I, et al.Serological and molecular investigation of Brucella species in dogs in Pakistan[J]. Pathogens, 2019, 8(4):294.
[22] 尚德秋, 李元凯.我国犬种菌布鲁氏菌病的调查研究[J]. 中华流行病学杂志, 1989, 10(1):24-29. SHANG D Q, LI Y K.Investigation of brucellosis caused by Brucella canis in China[J]. Chinese Journal of Epidemiology, 1989, 10(1):24-29.(in Chinese)
[23] 齐海霞, 张海云, 张宛婧, 等.宠物犬布鲁氏菌病的现状与防控[J]. 中国畜牧兽医文摘, 2013, 8:110-111. QI H X, ZHANG H Y, ZHANG W J, et al.Current situation and prevention of brucellosis in pet dogs[J]. Chinese Abstracts of Animal Husbandry and Veterinary Medicine, 2013, 8:110-111.(in Chinese)
[24] 黄迪海, 郭卉, 仉伟, 等.2016-2017年山东省济南市犬布鲁氏菌病血清流行病学调查[J]. 中国动物检疫, 2019, 36(7):15-19. HUANG D H, GUO H, ZHANG W, et al.Seroepidemiological investigation on brucellosis in canine in Jinan city of Shandong province from 2016 to 2017[J]. China Animal Health Inspection, 2019, 36(7):15-19.(in Chinese)
[25] 张沁涯.成都地区犬布氏杆菌病血清学调查及Real-time PCR检测方法的研究[D].雅安:四川农业大学, 2016. ZHANG Q Y.Serosurvey of canine brucellosis and developing a rapid diagnosis of Brucella by Real-time PCR[D].Ya'an:Sichuan Agricultural University, 2016.(in Chinese)
[26] RAJASHEKARA G, GLASNER J D, GLOVER D A, et al.Comparative whole-genome hybridization reveals genomic islands in Brucella species[J]. Journal of Bacteriology, 2004, 186(15):5040.
[27] CARMICHAEL L E, JOUBERT J C.Transmission of Brucella canis by contact exposure[J]. Cornell Veterinarian, 1988, 78(1):63.
[28] SERIKAWA T, MURAGUCHI T.Significance of urine in transmission of canine brucellosis[J]. Japanese Journal of Veterinary Science, 1979, 41(6):607-616.
[29] COSFORD K L.Brucella canis:An update on research and clinical management[J]. Canadian Veterinary Journal, 2018, 59(1):74-81.
[30] KIM S, WATARAI M, SUZUKI H, et al.Lipid raft microdomains mediate class A scavenger receptor-dependent infection of Brucella abortus[J]. Microbial Pathogenesis, 2004, 37(1):11-19.
[31] JIMENEZ D B M P, TERRAZA A, GROSS A, et al.Different responses of macrophages to smooth and rough Brucella spp.:Relationship to virulence[J]. Infection and Immunity, 2004, 72(4):2429.
[32] MARTÍN-MARTÍN A I, VIZCAINO N, FERNANDEZ-LAGO L.Cholesterol, ganglioside GM1 and class A scavenger receptor contribute to infection by Brucella ovis and Brucella canis in murine macrophages[J]. Microbes and Infection, 2010, 12(3):246-251.
[33] DETILLEUX P G, DEYOE B L, CHEVILLE N F.Entry and intracellular localization of Brucella spp.in Vero cells:Fluorescence and electron microscopy[J]. Veterinary Pathology, 1990, 27(5):317.
[34] DELPINO M V, FOSSATI C A, BALDI P C.Proinflammatory response of human osteoblastic cell lines and osteoblast-monocyte interaction upon infection with Brucella spp.[J]. Infection and Immunity, 2008, 77(3):984-995.
[35] CHACÓN-DÍAZ C, ALTAMIRANO-SILVA P, GONZALEZ-ESPINOZA G, et al.Brucella canis is an intracellular pathogen that induces a lower proinflammatory response than smooth zoonotic counterparts[J]. Infection and Immunity, 2015, 83(12):4861.
[36] STRANAHAN L W, KHALAF O H, GARCIA-GONZALEZ D G, et al.Characterization of Brucella canis infection in mice[J]. PLoS One, 2019, 14(6):e0218809.
[37] GVURANECZ M, SZEREDI L, RONAI Z, et al.Detection of Brucella canis-induced reproductive diseases in a kennel[J]. Journal of Veterinary Diagnostic Investigation, 2011, 23(1):143-147.
[38] BUHMANN G, PAUL F, HERBST W, et al.Canine brucellosis:Insights into the epidemiologic situation in Europe[J]. Frontiers in Veterinary Science, 2019, 6:151.
[39] GODFROID J, BECKMEN K, HELENA NYMO I.Removal of lipid from serum increases coherence between brucellosis rapid agglutination test and enzyme-linked immunosorbent assay in bears in Alaska, USA[J]. Journal of Wildlife Diseases, 2016, 52(4):912-915.
[40] KEID L B, DINIZ J A, OLIVEIRA T M, et al.Evaluation of an immunochromatographic test to the diagnosis of canine brucellosis caused by Brucella canis[J]. Reproduction in Domestic Animals, 2015, 50(6):939-944.
[41] MOL J P S, GUEDES A C B, ECKSTEIN C, et al.Diagnosis of canine brucellosis:Comparison of various serologic tests and PCR[J]. Journal of Veterinary Diagnostic Investigation, 2020, 32(1):77-86.
[42] HOLLETT R B.Canine brucellosis:Outbreaks and compliance[J]. Theriogenology, 2006, 66(3):575-587.
[43] DE OLIVEIRA M Z, VALE V, KEID L, et al.Validation of an ELISA method for the serological diagnosis of canine brucellosis due to Brucella canis[J]. Research in Veterinary Science, 2011, 90(3):425-431.
[44] ESCOBAR G I, BOERI E J, AYALA S M, et al.The feasibility of using antigens prepared with rough Brucella strains for diagnosis of canine brucellosis[J]. Revista Argentina de Microbiologia, 2010, 42(1):35-40.
[45] SANCHEZ-JIMENEZ M M, DE LA CUESTA ZULUAGA J J, GARCIA-MONTOYA G M, et al.Diagnosis of human and canine Brucella canis infection:Development and evaluation of indirect enzyme-linked immunosorbent assays using recombinant Brucella proteins[J]. Heliyon, 2020, 6(7):e04393.
[46] PAUL S, PEDDAVELACHAGIRI B V, GOGOI M, et al.Genome-wide unique insertion sequences among five Brucella species and demonstration of differential identification of Brucella by multiplex PCR assay[J]. Scientific Reports, 2020, 10(1):6368.
[47] 徐怀英, 黄迪海, 仉伟, 等.单重PCR鉴别犬4种布鲁氏菌检测方法的建立[J]. 中国动物检疫, 2020, 37(11):82-86. XU H Y, HUANG D H, ZHANG W, et al.Development of a single PCR assay for detection of four kinds of Brucella in dogs[J]. China Animal Health Inspection, 2020, 37(11):82-86.(in Chinese)
[48] 陈思.牛羊猪犬种布鲁氏菌多重PCR方法的建立及试剂盒研制[D].北京:中国人民解放军军事医学科学院, 2014. CHEN S.The establishment of multiplex PCR method and detection kit for Brucella abortus, Brucella melitensis, Brucella suis and Brucella canis[D].Beijing:Academy of Military Medical Sciences, 2014.(in Chinese)
[49] QIN L D, NAN W L, WANG Y, et al.A novel approach for detection of Brucella using a Real-time recombinase polymerase amplification assay[J]. Molecular and Cellular Probes, 2019, 48:101451.
[50] BATINGA M C A, DE LIMA J T R, GREGORI F, et al.Comparative application of IS711-based polymerase chain reaction (PCR) and loop-mediated isothermal amplification (LAMP) for canine brucellosis diagnosis[J]. Molecular and Cellular Probes, 2018, 39:1-6.
[51] ECKSTEIN C, MOL J P S, FAABIOLA B COSTA, et al.Brucella ovis mutant in ABC transporter protects against Brucella canis infection in mice and it is safe for dogs[J]. PLoS One, 2020, 15(4):e0231893.
[52] LIU Y, SUN J, PENG X, et al.Deletion of the LuxR-type regulator VjbR in Brucella canis affects expression of type Ⅳ secretion system and bacterial virulence, and the mutant strain confers protection against Brucella canis challenge in mice[J]. Microbial Pathogenesis, 2020, 139:103865
[53] STRANAHAN L W, CHAKI S P, GARCIA-GONZALEZ D G, et al.Evaluation of the efficacy of the Brucella canis RM6/66ΔvjbR vaccine candidate for protection against B.canis infection in mice[J]. mSphere, 2020, 5(3):172-200.
[54] CLAUSSE M, DIAZ A G, GHERSI G, et al.The vaccine candidate BLSOmp31 protects mice against Brucella canis infection[J]. Vaccine, 2013, 31(51):6129-6135.
[55] CLAUSSE M, DIAZ A G, IBANEZ A E, et al.Evaluation of the efficacy of outer membrane protein 31 vaccine formulations for protection against Brucella canis in BALB/c mice[J]. Clinical and Vaccine Immunology, 2014, 21(12):1689-1694.
[56] QIAN J, BU Z, LANG X, et al.A safe and molecular-tagged Brucella canis ghosts confers protection against virulent challenge in mice[J]. Veterinary Microbiology, 2017, 204:121.