河北省唐山市奶犊牛病毒性腹泻流行病学调查

doi:10.16431/j.cnki.1671-7236.2021.11.031

摘要/Abstract

摘要： 试验旨在掌握河北省唐山市13个县（市、区）奶牛场奶犊牛病毒性腹泻病原的流行状况，有效防控奶犊牛腹泻。采集唐山市不同地区38个奶牛场腹泻犊牛粪便样品788份，提取腹泻粪便样品中的基因组RNA。根据GenBank公布的牛病毒性腹泻病毒（Bovine viral diarrhea virus，BVDV）E2基因、牛轮状病毒（Bovine rotavirus，BRV）VP6基因、牛冠状病毒（Bovine coronavirus，BCV）N基因序列，利用Primer Premier 5.0软件分别设计特异性引物，采用PCR方法检测粪便样品中这3种基因，利用Excel 2007对不同地区、不同季节和混合感染病原检测结果进行汇总分析。在采样范围内的788份犊牛腹泻粪便样品中，BVDV、BRV和BCV阳性检出率分别为29.82%（235/788）、29.44%（232/788）和18.02%（142/788）；在所有被检地区，滦南县BVDV感染率最高，阳性检出率为45.38%（59/130），汉沽区BRV和BCV感染率最高，阳性检出率分别为55.00%（22/40）和37.50%（15/40）；从采样季节来看，春季、夏季和秋季BRV感染率最高，阳性检出率分别为27.71%（46/166）、39.58%（114/288）和19.89%（39/196），冬季则以BVDV感染为主，阳性检出率为52.17%（72/138）；从混合感染情况来看，以BRV＋BCV二重混合感染为主，感染率为8.37%（66/788）。河北省唐山市各地区腹泻犊牛群中均存在BVDV、BRV和BCV感染，以BVDV、BRV单一感染为主。

关键词: 奶犊牛; 病毒性腹泻; 牛病毒性腹泻病毒(BVDV); 牛轮状病毒(BRV); 牛冠状病毒(BCV); 流行病学调查

Abstract: The purpose of the experiment was to master the epidemic status of viral diarrhea pathogens of dairy calves in 13 counties (cities and districts) of Tangshan city, Hebei province, and to effectively prevent and control dairy calves diarrhea. 788 fecal samples of diarrhea calves from 38 dairy farms in different areas of Tangshan were collected to extract genomic RNA from diarrhea fecal samples. According to the E2 gene of Bovine viral diarrhea virus (BVDV), VP6 gene of Bovine rotavirus (BRV) and N gene of Bovine coronavirus (BCV) published in GenBank, specific primers were designed by Primer Premier 5.0 software, and the three genes in fecal samples were detected by PCR. The pathogen detection results of different areas, seasons and mixed infections were summarized and analyzed by Excel 2007. The positive rates of BVDV, BRV and BCV were 29.82% (235/788), 29.44% (232/788) and 18.02% (142/788), respectively. In all tested areas, Luannan county had the highest BVDV infection rate, with a positive detection rate of 45.38% (59/130). Hangu district had the highest BRV and BCV infection rates, with a positive detection rate of 55.00% (22/40) and 37.50% (15/40), respectively. From the sampling season, the BRV infection rate was the highest in spring, summer and autumn, with the positive detection rates of 27.71% (46/166), 39.58% (114/288) and 19.89% (39/196), respectively. In winter, BVDV infection was the main infection, with the positive detection rate of 52.17% (72/138). In terms of mixed infection, BRV+BCV double mixed infection was the main, and the infection rate was 8.37% (66/788). BVDV, BRV and BCV infection were found in diarrhea calves in Tangshan city, Hebei province, and the main infection was BVDV and BRV.

Key words: dairy calf; viral diarrhea; Bovine viral diarrhea virus (BVDV); Bovine rotavirus (BRV); Bovine coronavirus (BCV); epidemiological investigation

中图分类号:

S852.65⁺3

常丽云, 刘志勇, 秦建华, 赵月兰. 河北省唐山市奶犊牛病毒性腹泻流行病学调查[J]. 中国畜牧兽医, 2021, 48(11): 4213-4219.

CHANG Liyun, LIU Zhiyong, QIN Jianhua, ZHAO Yuelan. Epidemiological Investigation of Viral Diarrhea of Dairy Calves in Tangshan City, Hebei Province[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 4213-4219.

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