中国不同地区羊吕氏泰勒虫分子流行病学调查及遗传进化分析

doi:10.16431/j.cnki.1671-7236.2020.06.030

摘要/Abstract

摘要： 为了解吕氏泰勒虫在中国不同地区山羊和绵羊中的流行情况，本研究应用基于吕氏泰勒虫18S rRNA基因位点的PCR检测方法，对采自中国河南、甘肃、陕西、山西、贵州、云南、新疆7个地区的281份羊血液样品进行检测，并对阳性样品测序以进行序列分析。结果显示，羊吕氏泰勒虫总感染率为35.23%（99/281）。不同采样点羊吕氏泰勒虫感染率差异极显著（P<0.01），其中河南省羊吕氏泰勒虫感染率最高（98%，49/50），新疆最低（0）。绵羊和山羊吕氏泰勒虫感染率分别为51.67%（31/60）和30.77%（68/221），差异极显著（P<0.01）；放牧羊吕氏泰勒虫感染率（41.55%，86/207）极显著高于舍饲羊（17.57%，13/74）（P<0.01）；羊吕氏泰勒虫感染率在春、夏、秋及冬四季分别为56.00%（28/50）、42.75%（59/138）、9.43%（5/53）和17.50%（7/40），差异极显著（P<0.01）；≥ 12月龄和<12月龄羊吕氏泰勒虫感染率分别为33.50%（67/200）和39.51%（32/81），差异不显著（P>0.05）。此外，遗传进化分析表明，本研究获得的羊吕氏泰勒虫分离株与中国流行的吕氏泰勒虫分离株同源性高达99.60%以上，且位于同一分支上。本研究为进一步了解中国不同地区羊吕氏泰勒虫的流行现状及分布提供了重要参考依据。

关键词: 吕氏泰勒虫; 流行病学; 18S rRNA基因; 遗传进化分析; 绵羊; 山羊

Abstract: To understand the prevalence of T.luwenshuni in goats and sheep in different regions of China,in this study,281 sheep blood samples collected from 7 regions of Henan,Gansu,Shaanxi,Shanxi,Guizhou,Yunnan and Xinjiang were tested by PCR based on the 18S rRNA gene locus of T.luwenshuni,and positive samples were sequenced for sequence analysis.The results showed that the total infection rate of T.luwenshuni was 35.23% (99/281).The infection rate of T.luwenshuni differed significantly among sampling sites (P<0.01),among them,the infection rate of T.luwenshuni in Henan province was the highest (98%,49/50),and the lowest in Xinjiang (0).The infection rates of T.luwenshuni in sheep and goats were 51.67% (31/60) and 30.77% (68/221),respectively,and the differences were extremely significant (P<0.01).The infection rate of T.luwenshuni in grazing sheep (41.55%,86/207) was significantly higher than that in stabling sheep (17.57%,13/74) (P<0.01).The infection rates of T.luwenshuni were 56.00% (28/50),42.75% (59/138),9.43% (5/53) and 17.50% (7/40) in spring,summer,autumn and winter,respectively,and the differences were extremely significant (P<0.01).The infection rates of T.luwenshuni in sheep aged ≥ 12 months and <12 months were 33.50% (67/200) and 39.51% (32/81),respectively,with no significant difference (P>0.05).In addition,the genetic and evolutionary analysis indicated that the isolates of T.luwenshuni obtained from this study were more than 99.60% homologous to the isolates of T.luwenshuni isolated from China and they were located on the same cluster.This survey provided an important reference for further understanding of the prevalence and distribution of T.luwenshuni in sheep and goats in different regions of China.

Key words: T.luwenshuni; epidemiology; 18S rRNA gene; genetic and evolutionary analysis; sheep; goats

中图分类号:

S852.7⁺1

陈倩, 王坤轮, 闫亚群, 周永春, 赵聪, 菅复春, 王荣军, 张龙现, 宁长申. 中国不同地区羊吕氏泰勒虫分子流行病学调查及遗传进化分析[J]. 中国畜牧兽医, 2020, 47(6): 1902-1909.

CHEN Qian, WANG Kunlun, YAN Yaqun, ZHOU Yongchun, ZHAO Cong, JIAN Fuchun, WANG Rongjun, ZHANG Longxian, NING Changshen. Molecular Epidemiological Investigation and Genetic Evolution Analysis of Theileria luwenshuni in Sheep and Goats in Different Regions of China[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1902-1909.

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