牛源多杀性巴氏杆菌血清分型及毒力相关基因的检测研究

doi:10.16431/j.cnki.1671-7236.2020.06.031

摘要/Abstract

摘要： 为确定新疆北疆部分地区疑似病例中分离的牛源多杀性巴氏杆菌（Pasteurella multocida，Pm）流行的血清型、ST型及毒力相关基因的分布情况，本研究以分离的17株牛源Pm为研究对象，采用荚膜多重PCR分型法、脂多糖多重PCR分型法（LPS-mPCR）、多位点序列分型法（MLST）及PCR方法检测17株Pm分离株的荚膜型、脂多糖型、MLST型及7类共25个毒力相关基因的分布情况。结果显示，13株Pm的荚膜脂多糖型为A：L3型，ST型均为ST1型，4株Pm荚膜脂多糖型为B：L2型，ST型均为ST44；17株Pm毒力相关基因（exbB、exbD、fimA、fur、hgbA、hsf2、nanB、oma87、ompA、ompH、plpB、psl、ptfA、sodA、sodC、tonB和tbpA）的检出率高达100%，toxA基因的检出率为0。结果表明，从新疆北疆部分地区规模化牛场疑似病例中分离的Pm主要血清型为A：L3：ST1型。

关键词: 多杀性巴氏杆菌(Pm); 荚膜; 脂多糖; 多位点序列分型(MLST); 毒力相关基因

Abstract: In order to determine the distribution of serotypes,ST types and virulence related genes of Pasteurella multocida (Pm) isolated from suspected cases in Northern Xinjiang,17 strains of Pm isolated from cattle were studied in this study.The methods of capsule multiplex PCR,LPS multiplex PCR,multilocus sequence typing (MLST) and PCR were used to detect the capsule type,lipopolysaccharide type,MLST type and the distribution of 7 classes of 25 virulence related genes in 17 strains of Pm isolates.The results showed that 13 strains of Pm were A:L3,ST was ST1,4 strains of Pm were B:L2,ST was ST44.The detection rate of 17 Pm virulence related genes (exbB,exbD,fimA,fur,hgbA,hsf2,nanB,oma87,ompA,ompH,plpB,psl,ptfA,sodA,sodC,tonB and tbpA) was 100%,and the detection rate of toxA gene was 0.The results showed that the main serotype of Pm,which isolated from parts of northern Xinjiang large-scale cattle farms, was A:L3:ST1.

Key words: Pasteurella multocida (Pm); capsule; lipopolysaccharide; multilocus sequence typing (MLST); virulence-associated gene

中图分类号:

S852.61

邬琴, 张星星, 顾晓晓, 陶乔孝慈, 黄新, 韩猛立, 吴桐忠, 周霞, 钟发刚. 牛源多杀性巴氏杆菌血清分型及毒力相关基因的检测研究[J]. 中国畜牧兽医, 2020, 47(6): 1910-1920.

WU Qin, ZHANG Xingxing, GU Xiaoxiao, TAOQIAO Xiaoci, HUANG Xin, HAN Mengli, WU Tongzhong, ZHOU Xia, ZHONG Fagang. Study on Serotyping and Detection of Virulence-associated Genes of Pasteurella multocida Isolated from Bovine[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1910-1920.

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