›› 2008, Vol. 1 ›› Issue (10): 61-64.

• 遗传繁育 • 上一篇    下一篇

北京鸭肌肉生长抑制素基因多态与屠体性状关系分析

卢俊清1,黄苇1,赵楠2,王文武1,侯水生1 
  

  1. 1.中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室,北京 100193;2.西北农林科技大学动物科技学院,杨凌 712100
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-10-20 发布日期:2008-10-20

Analysis on Gene Polymorphism of Myostatin andCarcass Traits of Peking Duck

LU Junqing1, HUANG Wei1, ZHAO Nan2, WANG Wenwu1, HOU Shuisheng1
  

  1. 1.State Key Laboratory of Animal Nutrition Science, Institute of Animal Science, CAAS, Beijing, 100193, China; 2. College of Animal Scitech, Northwest A & F University, Yangling 712100,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-10-20 Published:2008-10-20

摘要: 肌肉生长抑制素(Myostatin,MSTN)基因在发育和成熟的骨骼肌中特异表达,对肌肉具有负调控作用。应用聚合酶链式反应单链构象多态性(PCR-SSCP)的方法,对6周龄北京鸭2个群体(Z2系和Z4系)共200个个体Myostatin基因5’调控区进行SNP分析,并对所发现的单核苷酸多态性与胴体重、胸肌重、腿肌重、全净膛重、皮脂重、腹脂重、骨架重、胸肌率、腿肌率、皮脂率、腹脂率进行了关联分析。结果发现,A1/A2位点基因型(AA和AB型)对6周龄Z2系和Z4系北京鸭的腹脂重(P<0.05)和胸肌重(P<0.10)有显著影响,因此可以将Myostatin基因作为影响北京鸭Z2系和Z4系腹脂重和胸肌重的候选基因。

关键词: 北京鸭; Myostatin; 基因多态

Abstract: Myostatin gene is expressed specifically in developing and mature skeletal muscle, and the expression products of myostatin gene inhibits muscle growth and differentiation. The polymorphism of the Peking duck myostatin gene was researched by PCR-SSCP. The SSCP was found in the myostatin gene promoter region. It showed two genotypes(AA,AB)in Z2 line and Z4 line of Peking ducks. The relationship between polymorphism in promoter and productive performance was analyzed. Variance analysis showed that AA genotype birds had significant higher weight of abdominal fat (P<0.05 )and breast meat(P<0.10) than AB genotype in different lines. Therefore, Myostatin gene could be taken as the candidate gene.

Key words: Peking duck; Myostatin; gene polymorphis

中图分类号: